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Offlinecalavera
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isolating a genotype
    #4834938 - 10/21/05 04:04 PM (18 years, 6 months ago)

hi

i understand how can u isolate a genotype on agar media, and i was wondering if the mycelium inside shroom caps, are also genotypes.

but if u isolate genotypes, you rather isolate the "ropyey" and well fruiting ones. how can you tell from a shroom cap, that it will contain a good genotype mycelia?

thanks for any info, thanks mulan


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OfflineRogerRabbitM
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Re: isolating a genotype [Re: calavera]
    #4835236 - 10/21/05 05:16 PM (18 years, 6 months ago)

Caps or stems can both be used for cloning. There is growing evidence however, that mushroom fruitbodies may contain more than one set of dikaryons. This means that more than one substrain may be present in the same fruitbody. That is why clones on agar often separate into individual sectors with a zone of inhibition between them.
RR


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Offlinecalavera
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Re: isolating a genotype [Re: RogerRabbit]
    #4836827 - 10/22/05 01:14 AM (18 years, 6 months ago)

so thats not the way:(

how about that:

when you make a honey jar, youi can see little "balls" of mycelia floating around.
can it be,that these balls are dikaryotic?

i dont think so, just want to be sure:)

so the only way to get my little genotype strains is to mess with the petri dishes.

thanks:)


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OfflineScourge
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Re: isolating a genotype [Re: calavera]
    #4858892 - 10/27/05 11:25 AM (18 years, 6 months ago)

yes, the "little 'balls'" are most definatly dikaryoitc. Best way to isolate monos IMO is to use extreamly diluted spore solution on agar. using that little solution in liquid is going to take forever to notice anything growing, and being liquid it probably encountered another compatable mono, and become dikaryotic.

Also. while I dont know that multipile dikaryotic collonies can't be in the same fruit body, I have never heard of it being a common happenstance. I've always beleave that when a clones fruit body seperates into diferant sectors, it was because of the enviromental condtions. If you have a book you could expain the details of muli-cultured fruit bodies, I would love to read it.


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InvisibleZen Peddler
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Re: isolating a genotype [Re: RogerRabbit]
    #4958273 - 11/19/05 11:57 PM (18 years, 5 months ago)

Growing evidence? Are you talking about phenotypes or genotypes?


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Invisibleohmatic
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Re: isolating a genotype [Re: Zen Peddler]
    #4962739 - 11/21/05 01:54 AM (18 years, 5 months ago)

dont be too scared by what roger mentioned, he basically said 'yes u can clone on agar - but ..'

the 'but ..' doesnt really matter for home cultivation as long as u do not want to
elevate it to a certain level, doing a tissue transfer to agar, then doing another
transfer of the 'ropey' (rhizomoprhic) as u said mycelium to another dish will leave u
with what u desire, a nice rhizomorphic culture based on the tissue of a fruitbody - a clone.

go for the agar, youll like it :smile:
peace ohm :mushroom2:


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Invisiblearmedia
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Registered: 11/03/01
Posts: 169
Re: isolating a genotype [Re: RogerRabbit]
    #4972709 - 11/23/05 02:48 PM (18 years, 5 months ago)

This is what is known as a genetic mosaic. It is apparently quite common in Armillaria species. I have not ever read about it happening with other species, but I wouldn't be at all surprised, since many times have I cloned a supposedly monotypic fruitbody to have it sector out. It's an area that is ripe for study.

That said, it is also my experience that "ropy", deeply rhizomorphic mycelium can often revert to linear growth after several transfers on agar, only to return to rhizomorphs when moved to a new medium. And some species (e.g. S. rugosoannulata) rarely, if ever, show rhizomorphs until moved onto "raw" substrates.

One thing this all means is that strains cloned from a fruitbody are very likely to be good fruiters, despite appearances on agar.

- A

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