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Offlinefarfromhome
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Syringe Contamination? - Spores101
    #4828713 - 10/20/05 08:26 AM (18 years, 3 months ago)

I have some syringes that look strange. I bought 4 of them from spores101. They all had this white milky stuff in them that looks similar to smoke from a cigarette. Here are some pictures:





I posted this on shroomtalk, as well. The only suggestion has been that it is spores that have already started growth. I bought 4 syringes, and inoculated using 1 of them. Every single cake was contamintated. The contamination started at the exact point that the solution entered the jar.

I am not a newbee and have done this 100+ times (5+ years). I posted an in depth thread (Possible Contaminated Syringes?) on shroomtalk.com, and I contacted spores101 regarding the problem and their response was:
Quote:

so what do you want ?

I am not sure what your trying to say

sounds like you dont know what your doin with the product you have
purchased




I replied to their email and am still patiently waiting on a second response, but would like to get another opinion on the issue.


Edited by farfromhome (10/20/05 10:33 AM)


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OfflinePrajna
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Re: Syringe Contamination? - Spores101 [Re: farfromhome]
    #4828731 - 10/20/05 08:34 AM (18 years, 3 months ago)

Doesn't look good bro, whatever it is. I can't imagine that it's myc growth inside the syringe, never heard of that.

Was this in the syringe from day one, or did it culture inside there?


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Offlineblackout
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Re: Syringe Contamination? - Spores101 [Re: Prajna]
    #4828808 - 10/20/05 09:08 AM (18 years, 3 months ago)

Happens all the time, just myc growing inside.
How did you go about innoculating the jars? did you wipe the needle and innoc holes before each injection?

I have had nothing but great dealings with www.spores101.com
great packaging and fast delivery


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InvisibleFooMan
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Re: Syringe Contamination? - Spores101 [Re: farfromhome]
    #4828815 - 10/20/05 09:13 AM (18 years, 3 months ago)

One thing to remember when dealing with spore vendors is that cultivation is ILLEGAL, so how you word your questions dictates the response you will get from them. Tell them you noticed contamination under the microscope, not that your jars are contaminated, catch my drift?. :wink:


--------------------

Quick WBS Prep


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OfflinePrajna
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Re: Syringe Contamination? - Spores101 [Re: blackout]
    #4828818 - 10/20/05 09:14 AM (18 years, 3 months ago)

Interesting blackout. Never heard of it before, what feeds the growth inside the syringe like that? How often does this happen? Still learning just want to know it if I ever see it in the future.


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InvisibleHolydiver
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Re: Syringe Contamination? - Spores101 [Re: farfromhome]
    #4828825 - 10/20/05 09:17 AM (18 years, 3 months ago)

It's simply germination of the spores within the water, a common thing and nothing to fret over. Looks clean to me.


--------------------
To find a place to live between the negatives and positives.


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Offlineblackout
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Re: Syringe Contamination? - Spores101 [Re: Holydiver]
    #4828926 - 10/20/05 09:54 AM (18 years, 3 months ago)

I figure the nutrients are tiny flecks of gill material that stick to the printing surface. Mushrooms are nutritious so there is probably a tiny amount sucked up into the syringe along with the spores. Maybe the spores even cannibalise themselves... I have had it in one vendor syringe before. I have probably had 10-15 syringes in my time. I see it here a lot, usually in the contam forum. Perfect for starting a LC!


Edited by blackout (10/20/05 09:54 AM)


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OfflinePrajna
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Re: Syringe Contamination? - Spores101 [Re: blackout]
    #4828950 - 10/20/05 10:02 AM (18 years, 3 months ago)

Yeah really, ready made LC. :thumbup:

That's exactly what I was wondering if the mushroom was cannibalizing itself for nutes, I'm going to have to visit the contam forum more often, interesting thread...


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InvisibleTien
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Re: Syringe Contamination? - Spores101 [Re: Prajna]
    #4828958 - 10/20/05 10:05 AM (18 years, 3 months ago)

Quote:

Prajna said:
Doesn't look good bro, whatever it is. I can't imagine that it's myc growth inside the syringe, never heard of that.





You'd be surprized...

you have nothing to worry about man, some of the spores germinated inside the syringe. That happened to me on several occasions.

Pluto


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Offlinefarfromhome
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Re: Syringe Contamination? - Spores101 [Re: Tien]
    #4829002 - 10/20/05 10:20 AM (18 years, 3 months ago)

Quote:

Was this in the syringe from day one, or did it culture inside there?



I don't know. I didn't examine them until having problems.

Quote:

Happens all the time, just myc growing inside.



This was my assumtion before I used the first syringe. I inoculated with one syringe and every single cake was contaminated. I've done this 100+ times with no contamination. Yes the needle was wiped, as well as the holes. The needle was fresh from the package, so that really shouldn't be necessary. The cakes were sterilized for 90 minutes at 15 psi. I do this to assure they are sterile. The process was outlined very well in my shroomtalk thread: Possible Contaminated Syringe with more pictures.

The one thing that I like to have and didn't in this case was a HEPA filter running in the room where the inoculation took place.

Quote:

One thing to remember when dealing with spore vendors is that cultivation is ILLEGAL, so how you word your questions...



I am aware of this and kept my emails to the company within the guidlines on their website. I didn't link to the thread at shroomtalk.com (see above) until the second email to them.

I am currently working toward a solution with customer service at spores101. I actually offered to try another one or send the syringes back, and the response I got back is the one that is listed above. I understand the main guy has a lot of life changes going on, right now. I'm being polite and patient in my emails.

I'm impressed! I should have posted my question here in the first place. I've never noticed the stuff in any syringes before, but I only examined them closely after being plagued with cobweb, green, and a little red shit. I've never seen anything like it in 5+ years (only a few episodes of green in already questionable circumstances + pictures here). Every single cake! No signs of cubensis mycelium, what-so-ever. Guess I've got some work cut out, now.

Thanks for the comments. I will update on the situation as it develops.


Edited by farfromhome (10/20/05 10:30 AM)


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InvisibleRoadkillM
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Re: Syringe Contamination? - Spores101 [Re: farfromhome]
    #4829016 - 10/20/05 10:29 AM (18 years, 3 months ago)

well...

like everyone else in this thread has already said...
It just spores that have germinated.
When a spore syringe is kept in warmer temps...it will germinate.

so now you have a mycelium syringe instead of a spore syringe...
which is a good thing.
they start colonization faster...after you inoculate your jars.


Quote:

The contamination started at the exact point that the solution entered the jar.




If you have contams at the inoculation points, like you say you have...
either the syringe was BAD...
or YOU didn't flame or wipe the syringe needle down with alcohol...
before inoculating your jars!~

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinefarfromhome
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Re: Syringe Contamination? - Spores101 [Re: Roadkill]
    #4829038 - 10/20/05 10:43 AM (18 years, 3 months ago)

Quote:

either the syringe was BAD... or YOU didn't flame or wipe the syringe needle down with alcohol...



As I stated, the needle was wiped with a cotton ball/alcohol. That is why I contacted the company about the problem. I still have 3 more syringes that I can experiment with that came in the same order. All of them show the same signs. BTW, they weren't kept in a warm environment, either.

Quote:

so now you have a mycelium syringe instead of a spore syringe... which is a good thing.



If it is in fact mycelium, then I completely agree. Back when I was doing this continuously, I always inoculated with live mycelium taken from fully colonized cakes. I never had contamination doing it that way either, and they do colonize much faster. On average, 2 weeks at the ideal temperature in my experience. I was still ordering from Psilocybe Fanaticus at that time, though.


Edited by farfromhome (10/20/05 10:44 AM)


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InvisibleRoadkillM
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Re: Syringe Contamination? - Spores101 [Re: farfromhome]
    #4829049 - 10/20/05 10:49 AM (18 years, 3 months ago)

Quote:

As I stated, the needle was wiped with a cotton ball/alcohol.




Sorry I missed that part...still drinking my first cup of coffee.
**bad Road**
lolzz

Then I would say that the syringe was already contaminated...
and should be replaced by the Vendor.


---

The syringe might have been stored in a warm place by the Vendor...
or during transportation to you.

A syringe kept in a cool dark place or a frig...
will not germinate...IME


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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