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InvisibleHippie3
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Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags * 1
    #4823969 - 10/19/05 06:56 AM (18 years, 5 months ago)

hi,
yer old friend Hippie here.
Just wanted to share something that's catching on over at mycotopia.net

a simple way to convert an empty syringe into an effective filter for air
to equalize pressure in sealed jars/bags
neutralizing the partial vacuum formed by sterilization
or the removal of fluid from a liquid culture jar.
see http://forums.mycotopia.net/showthread.php?t=5245
for the original thread info.
here's some pix-






ok,
that's how to make the airport itself, just pc it once to sterilize the interior
and it will filter air that passes thru it thereafter.


now how to use it on a jar of spore water or a liquid culture-
the lid is prepared thusly-









one can use this even to inject nutes/spores/air into sealed bottles of distilled water, sport drinks, etc. as needed.
many possible uses.
also see
http://forums.mycotopia.net/showthread.php?t=5177
and
http://forums.mycotopia.net/showthread.php?t=5001
and
http://forums.mycotopia.net/showthread.php?t=5212


here's how to use the airport to bubble air into a liquid culture to increase
dissolved gases and boost growth.






Quote:

I made a lid. i put 4 holes in it then put silicone over holes on both sides and allowed to dry for 24 hours. I then made my syringes i took a bunch of polyfil and stuffed it into 2 syringes and packed it down to make the filters then i took 1 syringe and siliconed an air tube into it to automat the process instead of blowing on one end(it makes it easier when bubbling the solution later) and i let it sit for 48 hours to totally let it dry it wasn't totally dry after 24 hours.

I started my LC by using a 250ml jar, i put 100ml water in the jar and 1 tablespoon of vanilla extract. i then put the lid on the jar seal side down b/c i want the jar to have a tight seal but left the ring loose on the jar. PC at 15 P.S.I. for 30 min then after PC i released the pressure from my cooker by the release valve and while hot i tightened the ring to keep seal. let cool. i then took a cotton swab with alcohol on it and put it over the hole and pierced it with one of the syringes to kill the vacuum in jar(or else it will suck all your spore syringe into the jar). i then used the same cotton swab and pierce through the silicone with my spore syringe. and allowed to grow. every 4 days i would stick my 2 syringes in to the holes. i put the non airtube syringe in first then the airtube one tipped the jar on its side and turned the pump on. the reason i like the air pump is that you can watch it bubble through the solution but also make sure you don't blow any into the other syringe.







i'm sure y'all can figure out where to go from here.
enjoy.
peace & love
-hip


--------------------
Admin @ mycotopia.net
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Edited by Hippie3 (10/19/05 12:20 PM)

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Invisibleohmatic
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4824029 - 10/19/05 07:49 AM (18 years, 5 months ago)

the pictures in this post do not work
and to view the pics @ mycotopia, one needs to be logged in.

fix your post - or its about useless.

btw, nice to see you still posting from time to time hip :smile:
peace ohm :mushroom2:


--------------------
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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: ohmatic] * 1
    #4824036 - 10/19/05 07:52 AM (18 years, 5 months ago)

bummer,
the pic upload limits here make it hard to upload all at once but i'll try to
upload what i can directly
and do more later
sorry


--------------------
Admin @ mycotopia.net
Mycotopia

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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4824067 - 10/19/05 08:10 AM (18 years, 5 months ago)

here's the first in the series


--------------------
Admin @ mycotopia.net
Mycotopia

Edited by Hippie3 (10/19/05 08:18 AM)

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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4824081 - 10/19/05 08:17 AM (18 years, 5 months ago)

ok,
here's the 2nd series-


and that's all i can upload now,
hit my daily limit,
sorry


--------------------
Admin @ mycotopia.net
Mycotopia

Edited by Hippie3 (10/19/05 08:19 AM)

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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4824151 - 10/19/05 09:01 AM (18 years, 5 months ago)

hi
i just adjusted the permissions at mycotopia
should allow un-registered visitors to see the pix now,
can you ?


--------------------
Admin @ mycotopia.net
Mycotopia

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InvisibleRoadkillM
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4824157 - 10/19/05 09:03 AM (18 years, 5 months ago)

Yes...I can see them now.

:smile:

Nice post hippie!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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Offlineblackout
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Roadkill] * 1
    #4824362 - 10/19/05 10:14 AM (18 years, 5 months ago)

It can be used in 2 ways for introducing air/bubbling a LC. Hippie shows a air pump pumping through the polyfil. If you stuck another syringe in you could suck air out, drawing air in through the port, this could be with your mouth or a vacuum cleaner with some sort of attachment (may be a bit noisy!). If using your mouth I would prefer sucking air out, rather than blowing in through the filter. Also you could soak the syringe with polyfil in alcohol and store in a bag while not in use.

The real bonus I see is no need for a filter on your LC jar. So you have the best of both worlds. You can shake it well to aerate and introduce fresh air every few days and shake it again to aerate and split up the myc. Agar had a similar solution but it had tubes attached which was a bit more tricky to source and build.

I had a more complicated design here
http://www.shroomery.org/forums/showflat.php?Cat=0&Board=Forum2&Number=4235588

They can also be used for PFjars, you can fully silicone the innoc holes on the lid and screw it down tight and introduce fresh air every few days

Edited by blackout (10/19/05 10:19 AM)

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Invisiblemskip23
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Roadkill] * 1
    #4824381 - 10/19/05 10:21 AM (18 years, 5 months ago)

Nice hip


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Invisiblebackupwards
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: mskip23] * 1
    #4825182 - 10/19/05 01:51 PM (18 years, 5 months ago)

it works great hippie, thanks for the tek hippie. i am adjusting my syringe bottles to use the airport. nice pics (spore).

:peace:

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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: backupwards] * 1
    #4828509 - 10/20/05 05:40 AM (18 years, 5 months ago)

here's a link-
http://forums.mycotopia.net/showthread.php?p=194953
to a thread demonstrating
how to use the airport system
to turn a store-bought un-opened bottle of fruitwater
into a liquid culture,
in this case a 2 yr old syringe of mexA was used
wuth success,
now have some ~60 syringefuls that can by syphoned out for use
simply by inserting an airport
and an empty clean syringe in the silicon ports.






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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 2
    #4828513 - 10/20/05 05:44 AM (18 years, 5 months ago)

btw
i'd request that
if you like this idea
plz bump up
my rating here,
some have trolled me in the past,
knocking it down.
i'd like my 5 shroom rating back, someday...
thx in advance.


--------------------
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Mycotopia

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InvisibleHolydiver
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4828546 - 10/20/05 06:37 AM (18 years, 5 months ago)

Very useful, Hippie3...thanks for sharing this.  :thumbup: :mushroom2:


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Holydiver] * 1
    #4828565 - 10/20/05 07:11 AM (18 years, 5 months ago)

my pleasure,
i'd appreciate feedback from folks who try the airport system
in various applications
either posted here or at mycotopia.net.


--------------------
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Invisibleagar
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4829291 - 10/20/05 12:14 PM (18 years, 5 months ago)





I have done something like that with LC's for a long time & it does work very well. I just use rubber tubing & rtv gasket sealer to seal the tubing into the lids.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar] * 1
    #4829326 - 10/20/05 12:23 PM (18 years, 5 months ago)

indeed, i've seen many variants
but i figure almost everyone has a few empty syringes lying around.
soon i'll post more pix showing how to create bulk spore water jars
that can fill 20-30 syringes at a time,
using the airport system.


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Offlineblackout
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4829379 - 10/20/05 12:35 PM (18 years, 5 months ago)

Agars jars work well but if you have say 20 jars on the go then you need 20 caps to be built. Using filtered syringes you simply poke 2 holes in each cap and but a blob of silicone on them. Then use just the one filtered syringe. I put bleach in my syringe and squirt it through the filter before putting it in my jar.


Edited by blackout (10/20/05 12:36 PM)

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Invisibleagar
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4829432 - 10/20/05 12:45 PM (18 years, 5 months ago)



I just use one of these uncommon items (Socorex auto-loading syringe).
Slide the feed tube into a LC rubber tube, agitate jar & pump away.
Makes it EASY as eating pie, to inoculate 40 or 50 spawn bags, or jars - at one setting.


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Invisibleagar
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: blackout] * 1
    #4829714 - 10/20/05 01:30 PM (18 years, 5 months ago)


Sun Tea jars (half & 1 gallon) can also be modified for LC's.
Simply alter the lid, remove spigot & replace with an injection port.


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InvisibleOlgualion
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar] * 1
    #4830177 - 10/20/05 03:28 PM (18 years, 5 months ago)

Showoffs :wink:

you guys are awesome! :smile:


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