Home | Community | Message Board

Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, HEPA Filter, Incense

Jump to first unread post. Pages: 1
Registered: 10/25/01
Posts: 130
Question: Laminar Etiquette
    #482410 - 12/06/01 10:12 PM (16 years, 5 months ago)

Advice needed.

I am a vetaran grower who has always used glove boxes for sterile work, with much success. I have recently built myself a HEPA flowhood and realized that I had no experience with one before, and I have several questions for anyone who does.

Is there some simple algorhythm i can use to determine the area of "clean" space in front of a filter (I have a 12"x24" with a 495CFM fan)?

Should I feel a solid flow of air from the filter, or is it actually meant to be gentle, and is gentle still sufficient to retain sterility?

Does having a LFH mean I should feel less paranoid about careful sterile practices, or simply that I now have a larger clean workspace to utilize and more freedom of movement?

I should mention that the idea of opening an agar plate in an open room terrifies me, and is the kind of thoght that keeps me up at night.

Any assurances/advice would be most appreciated.

- Agoraphobic In Albuquerque

Post Extras: Print Post  Remind Me! Notify Moderator
Registered: 10/12/01
Posts: 410
Last seen: 16 years, 1 month
Re: Question: Laminar Etiquette [Re: altarego]
    #482654 - 12/07/01 03:18 AM (16 years, 5 months ago)

thats a good question?? i used to work at this place making the silcone diaphrams. for navy suit controlers. i worked under a flowhood and i felt a steady but slow suction. you could only feel the suction like 2-3 inches away from the back wall.

real eyes, realize, real lies

Post Extras: Print Post  Remind Me! Notify Moderator

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: Question: Laminar Etiquette [Re: altarego]
    #482708 - 12/07/01 04:53 AM (16 years, 5 months ago)

Continue to follow scrupulous sterility techniques. The flow should be firm, but gentle. No wind tunnel here. You do have a larger area to work in and more freedom of movement. I suggest that you put a large see-thru hood/chamber around where the filtered air comes out. This decreases any particulate matter from floating in from the top.
I can't think of any formulas. sorry.


Post Extras: Print Post  Remind Me! Notify Moderator
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 2 months, 9 days
Re: Question: Laminar Etiquette [Re: altarego]
    #482740 - 12/07/01 05:55 AM (16 years, 5 months ago)

You should work very much in fron of the filter, as near as possible,perhaps 5-7 inches away.
There is a simple test for how far the laminar flow goes in to the room.
Light a cigarette or an incense smoke stick and hold it infront of the filter. You should be able to see the form of the air stream this way and be able to tell how far it reaches.

Post Extras: Print Post  Remind Me! Notify Moderator
Registered: 10/30/00
Posts: 219
Last seen: 16 years, 28 days
Re: Question: Laminar Etiquette [Re: altarego]
    #484750 - 12/09/01 03:54 AM (16 years, 5 months ago)

Some of the rules of laminar flow are different. The flow can blow contams from your hands or clothes so stay down wind from your work. Also let the flow run for about five minutes to clear the air before use. Flame sterilization of tools does not work well with laminar flow because the flame disrupts the flow so either keep the flame outside the hood or use an electric incinerator. Laminar flow is great when it works. You can test how well it works by exposing agar plates to the flow.

Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, HEPA Filter, Incense

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Glovebox critiscism/questions? socratesmind 912 2 10/07/02 11:23 PM
by Green_Velvet
* 24''x24'' Flow Hood Questions
( 1 2 all )
Cow Shit Collector 8,109 26 02/07/05 01:27 PM
by Arp
* Laminar Flow Q - Maintaining Sterility? quart_o_cunt 1,525 8 03/04/02 05:38 PM
by cookiewhore
( 1 2 3 4 5 6 7 8 9 all )
psilocybeen 21,980 165 02/09/08 04:45 AM
by ZeropointMind
* Laminar Air Flow Calibration With A Candle agar 1,440 5 01/19/05 09:40 AM
by Spores_Direct
* Sterilizing Syringes Question Anonymous 1,022 10 04/01/02 08:41 PM
by Anonymous
* Huge Laminar Flow Hood? Pugslee_Atoms420 1,840 8 04/12/03 12:17 PM
by debianlinux
* Worm Poo Questions tom 1,909 13 06/27/01 08:08 PM
by tom

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
957 topic views. 1 members, 5 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.017 seconds spending 0.004 seconds on 16 queries.