|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags 1
#4823969 - 10/19/05 06:56 AM (18 years, 6 months ago) |
|
|
hi, yer old friend Hippie here. Just wanted to share something that's catching on over at mycotopia.net
a simple way to convert an empty syringe into an effective filter for air to equalize pressure in sealed jars/bags neutralizing the partial vacuum formed by sterilization or the removal of fluid from a liquid culture jar. see http://forums.mycotopia.net/showthread.php?t=5245 for the original thread info. here's some pix-  
ok, that's how to make the airport itself, just pc it once to sterilize the interior and it will filter air that passes thru it thereafter.
now how to use it on a jar of spore water or a liquid culture- the lid is prepared thusly-
one can use this even to inject nutes/spores/air into sealed bottles of distilled water, sport drinks, etc. as needed. many possible uses. also see http://forums.mycotopia.net/showthread.php?t=5177 and http://forums.mycotopia.net/showthread.php?t=5001 and http://forums.mycotopia.net/showthread.php?t=5212
here's how to use the airport to bubble air into a liquid culture to increase dissolved gases and boost growth.
Quote:
I made a lid. i put 4 holes in it then put silicone over holes on both sides and allowed to dry for 24 hours. I then made my syringes i took a bunch of polyfil and stuffed it into 2 syringes and packed it down to make the filters then i took 1 syringe and siliconed an air tube into it to automat the process instead of blowing on one end(it makes it easier when bubbling the solution later) and i let it sit for 48 hours to totally let it dry it wasn't totally dry after 24 hours.
I started my LC by using a 250ml jar, i put 100ml water in the jar and 1 tablespoon of vanilla extract. i then put the lid on the jar seal side down b/c i want the jar to have a tight seal but left the ring loose on the jar. PC at 15 P.S.I. for 30 min then after PC i released the pressure from my cooker by the release valve and while hot i tightened the ring to keep seal. let cool. i then took a cotton swab with alcohol on it and put it over the hole and pierced it with one of the syringes to kill the vacuum in jar(or else it will suck all your spore syringe into the jar). i then used the same cotton swab and pierce through the silicone with my spore syringe. and allowed to grow. every 4 days i would stick my 2 syringes in to the holes. i put the non airtube syringe in first then the airtube one tipped the jar on its side and turned the pump on. the reason i like the air pump is that you can watch it bubble through the solution but also make sure you don't blow any into the other syringe.
i'm sure y'all can figure out where to go from here. enjoy. peace & love -hip
-------------------- Admin @ mycotopia.net Mycotopia
Edited by Hippie3 (10/19/05 12:20 PM)
|
ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4824029 - 10/19/05 07:49 AM (18 years, 6 months ago) |
|
|
the pictures in this post do not work and to view the pics @ mycotopia, one needs to be logged in.
fix your post - or its about useless.
btw, nice to see you still posting from time to time hip  peace ohm
--------------------
MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: ohmatic] 1
#4824036 - 10/19/05 07:52 AM (18 years, 6 months ago) |
|
|
bummer, the pic upload limits here make it hard to upload all at once but i'll try to upload what i can directly and do more later sorry
-------------------- Admin @ mycotopia.net Mycotopia
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4824067 - 10/19/05 08:10 AM (18 years, 6 months ago) |
|
|
here's the first in the series    
-------------------- Admin @ mycotopia.net Mycotopia
Edited by Hippie3 (10/19/05 08:18 AM)
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4824081 - 10/19/05 08:17 AM (18 years, 6 months ago) |
|
|
ok, here's the 2nd series-
     
and that's all i can upload now, hit my daily limit, sorry
-------------------- Admin @ mycotopia.net Mycotopia
Edited by Hippie3 (10/19/05 08:19 AM)
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4824151 - 10/19/05 09:01 AM (18 years, 6 months ago) |
|
|
hi i just adjusted the permissions at mycotopia should allow un-registered visitors to see the pix now, can you ?
-------------------- Admin @ mycotopia.net Mycotopia
|
Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4824157 - 10/19/05 09:03 AM (18 years, 6 months ago) |
|
|
Yes...I can see them now.

Nice post hippie!~
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 11 days
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Roadkill] 1
#4824362 - 10/19/05 10:14 AM (18 years, 6 months ago) |
|
|
It can be used in 2 ways for introducing air/bubbling a LC. Hippie shows a air pump pumping through the polyfil. If you stuck another syringe in you could suck air out, drawing air in through the port, this could be with your mouth or a vacuum cleaner with some sort of attachment (may be a bit noisy!). If using your mouth I would prefer sucking air out, rather than blowing in through the filter. Also you could soak the syringe with polyfil in alcohol and store in a bag while not in use.
The real bonus I see is no need for a filter on your LC jar. So you have the best of both worlds. You can shake it well to aerate and introduce fresh air every few days and shake it again to aerate and split up the myc. Agar had a similar solution but it had tubes attached which was a bit more tricky to source and build.
I had a more complicated design here http://www.shroomery.org/forums/showflat.php?Cat=0&Board=Forum2&Number=4235588
They can also be used for PFjars, you can fully silicone the innoc holes on the lid and screw it down tight and introduce fresh air every few days
Edited by blackout (10/19/05 10:19 AM)
|
mskip23
Can It All Be So Simple!


Registered: 06/08/05
Posts: 1,522
Loc: Philly
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Roadkill] 1
#4824381 - 10/19/05 10:21 AM (18 years, 6 months ago) |
|
|
Nice hip
-------------------- url=https://files.shroomery.org/files/05-26/988356075-Picture-278.gif] [/url
|
backupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: mskip23] 1
#4825182 - 10/19/05 01:51 PM (18 years, 6 months ago) |
|
|
it works great hippie, thanks for the tek hippie. i am adjusting my syringe bottles to use the airport. nice pics (spore).
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: backupwards] 1
#4828509 - 10/20/05 05:40 AM (18 years, 6 months ago) |
|
|
here's a link- http://forums.mycotopia.net/showthread.php?p=194953 to a thread demonstrating how to use the airport system to turn a store-bought un-opened bottle of fruitwater into a liquid culture, in this case a 2 yr old syringe of mexA was used wuth success, now have some ~60 syringefuls that can by syphoned out for use simply by inserting an airport and an empty clean syringe in the silicon ports.
-------------------- Admin @ mycotopia.net Mycotopia
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 2
#4828513 - 10/20/05 05:44 AM (18 years, 6 months ago) |
|
|
btw i'd request that if you like this idea plz bump up my rating here, some have trolled me in the past, knocking it down. i'd like my 5 shroom rating back, someday... thx in advance.
-------------------- Admin @ mycotopia.net Mycotopia
|
Holydiver
Stranger



Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4828546 - 10/20/05 06:37 AM (18 years, 6 months ago) |
|
|
Very useful, Hippie3...thanks for sharing this.
-------------------- To find a place to live between the negatives and positives.
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Holydiver] 1
#4828565 - 10/20/05 07:11 AM (18 years, 6 months ago) |
|
|
my pleasure, i'd appreciate feedback from folks who try the airport system in various applications either posted here or at mycotopia.net.
-------------------- Admin @ mycotopia.net Mycotopia
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4829291 - 10/20/05 12:14 PM (18 years, 6 months ago) |
|
|
   
  
I have done something like that with LC's for a long time & it does work very well. I just use rubber tubing & rtv gasket sealer to seal the tubing into the lids.
--------------------
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar] 1
#4829326 - 10/20/05 12:23 PM (18 years, 6 months ago) |
|
|
indeed, i've seen many variants but i figure almost everyone has a few empty syringes lying around. soon i'll post more pix showing how to create bulk spore water jars that can fill 20-30 syringes at a time, using the airport system.
-------------------- Admin @ mycotopia.net Mycotopia
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 11 days
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4829379 - 10/20/05 12:35 PM (18 years, 6 months ago) |
|
|
Agars jars work well but if you have say 20 jars on the go then you need 20 caps to be built. Using filtered syringes you simply poke 2 holes in each cap and but a blob of silicone on them. Then use just the one filtered syringe. I put bleach in my syringe and squirt it through the filter before putting it in my jar.
Edited by blackout (10/20/05 12:36 PM)
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] 1
#4829432 - 10/20/05 12:45 PM (18 years, 6 months ago) |
|
|

I just use one of these uncommon items (Socorex auto-loading syringe). Slide the feed tube into a LC rubber tube, agitate jar & pump away. Makes it EASY as eating pie, to inoculate 40 or 50 spawn bags, or jars - at one setting.
--------------------
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: blackout] 1
#4829714 - 10/20/05 01:30 PM (18 years, 6 months ago) |
|
|
 Sun Tea jars (half & 1 gallon) can also be modified for LC's. Simply alter the lid, remove spigot & replace with an injection port.
--------------------
|
Olgualion
Shaman-In-Training


Registered: 11/13/01
Posts: 1,253
Loc: Currently Earth...
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar] 1
#4830177 - 10/20/05 03:28 PM (18 years, 6 months ago) |
|
|
Showoffs 
you guys are awesome!
-------------------- Study the past... See the future...
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: blackout]
#4830673 - 10/20/05 04:48 PM (18 years, 6 months ago) |
|
|
Quote:
blackout said: Agars jars work well but if you have say 20 jars on the go then you need 20 caps to be built. Using filtered syringes you simply poke 2 holes in each cap and but a blob of silicone on them. Then use just the one filtered syringe. I put bleach in my syringe and squirt it through the filter before putting it in my jar.
true enough, hard to get much simpler/easier/cheaper than stuffing an old syringe with polyfil, poking a hole in a lid with an icepick and applying a blob of silicon adhesive. that's it, do it for a penny a jar, nothing permanent so jars can be stacked anywhere. lay jar on side, upside down- matters not. shake a LC until the tissue fragments, no need for glass or magnets. but one big advantage- you can place the silicon on a plastic bottle never opening it and slide the needle in to inoculate. you could inject apple juice into a gallon jug of distilled water, right from the unopened jar of juice to the unopened jug of water to create a nutrient solution then inject a few spores- voila! a heat-free flowhood-less way to make a gallon of liquid inoculant at a time for mere pennies in just a few minutes wih very few supplies needed.
-------------------- Admin @ mycotopia.net Mycotopia
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#4830830 - 10/20/05 05:11 PM (18 years, 6 months ago) |
|
|
here;s another thread to check out- http://forums.mycotopia.net/showthread.php?p=194915
one thing the airport system makes feasible is 'canning' nutrient solution the old fashioned way- lids with siliconed holes, rubber-side down so it seals fully as it cools. once cooled/sealed inside the jar will be a partial vacuum. if carefully inoculated to preserve most of the vacuum, his experiment seems to indicate that mycellia grows faster in the low pressure environment. this is still bleeding edge stuff with a need for more evidence/confirmation but if the phenomenon proves real/consistent/significant it could change how we do LC's, dramatically speeding up the growth of tissue in liquids. worth a few more tests, imho.
-------------------- Admin @ mycotopia.net Mycotopia
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 11 days
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#4832999 - 10/21/05 02:30 AM (18 years, 6 months ago) |
|
|
Yes, I was talking to you about the apple juice at mycotpia. You can easily put a needle in the perforated hole on the tetra pack. Juice is usually 10-12% sugar so add 2 parts water to one juice, though weak LCs work fine too. Some sports drinks are now 3-5% sugars instead of the usual 10-12%. Lately I have been working in a/c bags as a cheap glove box. If you made a jar of 50/50 water/honey and heated it up it will be fluid enough to pour into a large bottle of water in a glovebox.
If working with vacuum just make sure you dont lose your whole syringe when you inject.
I do most of my sterilizing in another house, so transporting LCs used to be a big problem for me, it does solve it.
I have had great success adding large amounts of LC to grains and have some projects I will be working on soon that need a lot of LC.
|
Tweexican
Clit Commander

Registered: 11/06/03
Posts: 657
Last seen: 5 years, 10 months
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar]
#4833434 - 10/21/05 10:17 AM (18 years, 6 months ago) |
|
|
Quote:
agar said:

I just use one of these uncommon items (Socorex auto-loading syringe). Slide the feed tube into a LC rubber tube, agitate jar & pump away. Makes it EASY as eating pie, to inoculate 40 or 50 spawn bags, or jars - at one setting.
where did you get that gun???!!!
--------------------
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
#4836334 - 10/21/05 09:37 PM (18 years, 6 months ago) |
|
|
eBay for $26 plus S/H. They run arounf $140 retail & depending on what fittings you get with it. Soccorex is the best in the world.
--------------------
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#4844780 - 10/24/05 06:52 AM (18 years, 6 months ago) |
|
|
a couple more pix showing airport in action bubbling air into a liquid culture [karo jar]
-------------------- Admin @ mycotopia.net Mycotopia
|
Tweexican
Clit Commander

Registered: 11/06/03
Posts: 657
Last seen: 5 years, 10 months
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#4844796 - 10/24/05 07:17 AM (18 years, 6 months ago) |
|
|
thats a pretty dark karo jar... did you use malt extract in that at all hip?
--------------------
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
#4844849 - 10/24/05 08:09 AM (18 years, 6 months ago) |
|
|
no, but instead vanilla extract was added. for some info on vanilla see laz's vanilla tek.
-------------------- Admin @ mycotopia.net Mycotopia
Edited by Hippie3 (10/24/05 08:23 AM)
|
Tweexican
Clit Commander

Registered: 11/06/03
Posts: 657
Last seen: 5 years, 10 months
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#4896283 - 11/05/05 01:38 AM (18 years, 6 months ago) |
|
|
In the picture with the airport being used to pull sterile distilled water... was the water used to make syringes or straight for liquid culture use???
--------------------
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
#4896597 - 11/05/05 05:01 AM (18 years, 6 months ago) |
|
|
it was used to make syringes in that particular case. the bottle of fruitwater was used as a liquid culture [mexicana A]
-------------------- Admin @ mycotopia.net Mycotopia
|
Tweexican
Clit Commander

Registered: 11/06/03
Posts: 657
Last seen: 5 years, 10 months
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#4897126 - 11/05/05 11:29 AM (18 years, 6 months ago) |
|
|
so was it, pull water from bottle, and hydrate spores with a brand new syringe?
if so, how was the contam turn out?
--------------------
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
#4897153 - 11/05/05 11:38 AM (18 years, 6 months ago) |
|
|
correct. no contams.
-------------------- Admin @ mycotopia.net Mycotopia
|
Tweexican
Clit Commander

Registered: 11/06/03
Posts: 657
Last seen: 5 years, 10 months
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#4897716 - 11/05/05 03:37 PM (18 years, 6 months ago) |
|
|
how exactly was the transfer of spores to the syringe done?
shot glass tek?
--------------------
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
#4897755 - 11/05/05 03:48 PM (18 years, 6 months ago) |
|
|
no, the print was placed inside small ziplock bag and water was injected, stirred to mix then sucked back into syringe
see http://forums.mycotopia.net/showthread.php?t=5782
-------------------- Admin @ mycotopia.net Mycotopia
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#5404193 - 03/15/06 03:45 PM (18 years, 2 months ago) |
|
|
a new experiment using the airport instead of a dry verm. barrier in cake culture see http://forums.mycotopia.net/showthread.php?p=231866
-------------------- Admin @ mycotopia.net Mycotopia
|
Dragonaut

Registered: 06/24/04
Posts: 6,219
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#5411154 - 03/17/06 01:47 AM (18 years, 2 months ago) |
|
|
This thread needs thumbnails.
--------------------
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Dragonaut]
#5428746 - 03/22/06 05:58 AM (18 years, 2 months ago) |
|
|
ask and ye shall receive.

-------------------- Admin @ mycotopia.net Mycotopia
|
JSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 6 months, 2 days
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#5437878 - 03/24/06 10:56 AM (18 years, 1 month ago) |
|
|
are the spore bags being sterilized in anyway before puttig the spores in and conpleting the sealing process.
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
#5439087 - 03/24/06 05:54 PM (18 years, 1 month ago) |
|
|
nope sterile enough from the factory although one could swab with rubbing alky if desired
-------------------- Admin @ mycotopia.net Mycotopia
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
#5440389 - 03/25/06 06:57 AM (18 years, 1 month ago) |
|
|
permanent endless supply of sterlie air sometimes one needs a source of sterile air. here's a simple way. quart jar fitted with twin silicon ported lid about 1/2 inch of water in bottom run the pc to sterilize insides then place airport in one hole to equalize pressure. empty syringe inserted thru other hole can then be filled with sterile air as needed. the polyfil syringe will filter replacement air maintaining equal pressure


-------------------- Admin @ mycotopia.net Mycotopia
Edited by Hippie3 (03/29/06 06:10 PM)
|
Pinback
Stranger


Registered: 07/20/02
Posts: 836
Loc: Europe
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#5440401 - 03/25/06 07:26 AM (18 years, 1 month ago) |
|
|
Or one can just put the needle tip in a flame and pull back the plunger.
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Pinback]
#5440493 - 03/25/06 09:09 AM (18 years, 1 month ago) |
|
|
i sure wouldn't rely on that. flame doesn't instantly kill every microbe sucked thru it. try that and shoot the air on some agar... also flame is inappropriate for some situations, such as a lysol-laden glovebox.
-------------------- Admin @ mycotopia.net Mycotopia
Edited by Hippie3 (03/25/06 10:08 AM)
|
Jaeger
Dreamer
Registered: 10/01/05
Posts: 960
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#5440638 - 03/25/06 10:56 AM (18 years, 1 month ago) |
|
|
Good idea hippie, but what is the point of the water in there?
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Jaeger]
#5443411 - 03/26/06 07:32 AM (18 years, 1 month ago) |
|
|
steam is far more effective than dry heat. pc it, steam kills everything inside. baking it hot enough to do that would ruin the lid's rubber seal. proly just a spoonful inside is plenty.
-------------------- Admin @ mycotopia.net Mycotopia
|
Tweexican
Clit Commander

Registered: 11/06/03
Posts: 657
Last seen: 5 years, 10 months
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#5456373 - 03/29/06 06:42 PM (18 years, 1 month ago) |
|
|
this seems like a lot of trouble.
i liked what you did with the store bought flavored water for lc use. ingenius.
what use does an airport serve if you have to go to so much trouble just to keep up with the maintenence of oxygenating your jars?
in this case instead of trying to get "sterile air", wouldn't it be easier to just use a polyfil filter for your jars?
--------------------
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
#5458249 - 03/30/06 05:17 AM (18 years, 1 month ago) |
|
|
it's no great trouble to squeeze out a blob of silicon or two. it's no trouble to suck air in a syringe. i'm not trying to sell you an air kit, if you don't want to try it, don't. someone somewhere will, and that is good enough reason to post it. see this thread for current example of trouble some folks have with polyfil- wet polyfil one last thought- the easy way isn't always best. polyfil can get wet and let contams thru. esp. when doing liquid cultures. this air supply allows one to give LCs a hard shake [try that with a polyfil filtered lid] to break up mycellia, inject sterile air. one LC can make many syringefuls of inoculant, making the 'trouble' rather worthwhile. some folks have trouble finding polyfil, maybe they live in thailand in a hut on edge of jungle with no walmart in sight. maybe they just want to experiment with something new to see if it can be done.
Edited by Hippie3 (03/30/06 05:43 AM)
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
#5458262 - 03/30/06 05:27 AM (18 years, 1 month ago) |
|
|
Quote:
Tweexican said:
i liked what you did with the store bought flavored water for lc use. ingenius.
speaking of which- that culture is now 6 months old and still doing well, just filled another syringe of mexA tissue a few days ago. growth has never gotten dense but there's only a 2% sugars content. drawing the LC out of the lower airport works like a charm as gravity settles the tissue to the bottom so you can suck it right into the needle, air bubbles in the upper airport to compensate the volume lost. i'm seriously thinking about trying to 'feed' this LC by injecting a little fresh karo water.
-------------------- Admin @ mycotopia.net Mycotopia
|
JSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 6 months, 2 days
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#5500408 - 04/10/06 04:34 PM (18 years, 1 month ago) |
|
|
Heres an airport I made.
 
Its an air valve from the plumbing section that was a hell of a tight fit but threaded onto a polyfilled syringe so that the airpump plugs right into it.
I Pc my airports everytime I use them is this necessary?
|
Hippie3
mycotopiate


Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
#5500416 - 04/10/06 04:37 PM (18 years, 1 month ago) |
|
|
no, i just re=pc them after a few uses.
-------------------- Admin @ mycotopia.net Mycotopia
|
consciousallah
Golden Student

Registered: 02/26/24
Posts: 11
Last seen: 6 hours, 19 minutes
|
Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
#28736950 - 04/14/24 03:57 AM (1 month, 3 days ago) |
|
|
Working on something similar.
|
|