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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: blackout]
    #4830673 - 10/20/05 04:48 PM (18 years, 6 months ago)

Quote:

blackout said:
Agars jars work well but if you have say 20 jars on the go then you need 20 caps to be built. Using filtered syringes you simply poke 2 holes in each cap and but a blob of silicone on them. Then use just the one filtered syringe. I put bleach in my syringe and squirt it through the filter before putting it in my jar.







true enough,
hard to get much simpler/easier/cheaper
than stuffing an old syringe with polyfil,
poking a hole in a lid with an icepick
and applying a blob of silicon adhesive.
that's it,
do it for a penny a jar,
nothing permanent so jars can be stacked anywhere.
lay jar on side, upside down- matters not.
shake a LC until the tissue fragments,
no need for glass or magnets.
but one big advantage-
you can place the silicon on a plastic bottle
never opening it
and slide the needle in to inoculate.
you could inject apple juice into a gallon jug of distilled water,
right from the unopened jar of juice to the unopened jug of water
to create a nutrient solution
then inject a few spores-
voila! a heat-free flowhood-less way
to make a gallon of liquid inoculant at a time for mere pennies
in just a few minutes wih very few supplies needed.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4830830 - 10/20/05 05:11 PM (18 years, 6 months ago)

here;s another thread to check out-
http://forums.mycotopia.net/showthread.php?p=194915

one thing the airport system makes feasible
is 'canning' nutrient solution the old fashioned way-
lids with siliconed holes, rubber-side down
so it seals fully as it cools.
once cooled/sealed
inside the jar will be a partial vacuum.
if carefully inoculated to preserve most of the vacuum,
his experiment seems to indicate
that mycellia grows faster in the low pressure environment.
this is still bleeding edge stuff
with a need for more evidence/confirmation
but if the phenomenon proves real/consistent/significant
it could change how we do LC's,
dramatically speeding up the growth of tissue in liquids.
worth a few more tests, imho.


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Offlineblackout
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4832999 - 10/21/05 02:30 AM (18 years, 6 months ago)

Yes, I was talking to you about the apple juice at mycotpia. You can easily put a needle in the perforated hole on the tetra pack. Juice is usually 10-12% sugar so add 2 parts water to one juice, though weak LCs work fine too. Some sports drinks are now 3-5% sugars instead of the usual 10-12%. Lately I have been working in a/c bags as a cheap glove box. If you made a jar of 50/50 water/honey and heated it up it will be fluid enough to pour into a large bottle of water in a glovebox.

If working with vacuum just make sure you dont lose your whole syringe when you inject.

I do most of my sterilizing in another house, so transporting LCs used to be a big problem for me, it does solve it.

I have had great success adding large amounts of LC to grains and have some projects I will be working on soon that need a lot of LC.

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OfflineTweexican
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar]
    #4833434 - 10/21/05 10:17 AM (18 years, 6 months ago)

Quote:

agar said:


I just use one of these uncommon items (Socorex auto-loading syringe).
Slide the feed tube into a LC rubber tube, agitate jar & pump away.
Makes it EASY as eating pie, to inoculate 40 or 50 spawn bags, or jars - at one setting.




where did you get that gun???!!!


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Invisibleagar
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4836334 - 10/21/05 09:37 PM (18 years, 6 months ago)

eBay for $26 plus S/H. They run arounf $140 retail & depending on what fittings you get with it. Soccorex is the best in the world.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4844780 - 10/24/05 06:52 AM (18 years, 6 months ago)

a couple more pix
showing airport in action
bubbling air into a liquid culture [karo jar]



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OfflineTweexican
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4844796 - 10/24/05 07:17 AM (18 years, 6 months ago)

thats a pretty dark karo jar... did you use malt extract in that at all hip?


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4844849 - 10/24/05 08:09 AM (18 years, 6 months ago)

no,
but instead
vanilla extract was added.
for some info on vanilla
see
laz's vanilla tek.


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Edited by Hippie3 (10/24/05 08:23 AM)

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OfflineTweexican
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4896283 - 11/05/05 01:38 AM (18 years, 6 months ago)

In the picture with the airport being used to pull sterile distilled water... was the water used to make syringes or straight for liquid culture use???


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4896597 - 11/05/05 05:01 AM (18 years, 6 months ago)

it was used to make syringes in that particular case.
the bottle of fruitwater was used as a liquid culture [mexicana A]


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OfflineTweexican
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4897126 - 11/05/05 11:29 AM (18 years, 6 months ago)

so was it, pull water from bottle, and hydrate spores with a brand new syringe?

if so, how was the contam turn out?


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4897153 - 11/05/05 11:38 AM (18 years, 6 months ago)

correct.
no contams.


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OfflineTweexican
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4897716 - 11/05/05 03:37 PM (18 years, 6 months ago)

how exactly was the transfer of spores to the syringe done?

shot glass tek?


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4897755 - 11/05/05 03:48 PM (18 years, 6 months ago)

no,
the print was placed inside small ziplock bag
and water was injected, stirred to mix then sucked back into syringe

see
http://forums.mycotopia.net/showthread.php?t=5782


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5404193 - 03/15/06 03:45 PM (18 years, 2 months ago)

a new experiment using
the airport instead of a
dry verm. barrier in cake culture
see
http://forums.mycotopia.net/showthread.php?p=231866


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InvisibleDragonaut

Registered: 06/24/04
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5411154 - 03/17/06 01:47 AM (18 years, 2 months ago)

This thread needs thumbnails. :smirk:


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Dragonaut]
    #5428746 - 03/22/06 05:58 AM (18 years, 2 months ago)

ask
and ye shall receive.




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OfflineJSshroom
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5437878 - 03/24/06 10:56 AM (18 years, 1 month ago)

are the spore bags being sterilized in anyway before puttig the spores in and conpleting the sealing process.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
    #5439087 - 03/24/06 05:54 PM (18 years, 1 month ago)

nope
sterile enough from the factory
although one could swab with rubbing alky if desired


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
    #5440389 - 03/25/06 06:57 AM (18 years, 1 month ago)

permanent endless supply of sterlie air
sometimes one needs a source of sterile air.
here's a simple way.
quart jar fitted with twin silicon ported lid
about 1/2 inch of water in bottom
run the pc to sterilize insides
then place airport in one hole to equalize pressure.
empty syringe inserted thru other hole
can then be filled with sterile air as needed.
the polyfil syringe will filter replacement air
maintaining equal pressure







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Edited by Hippie3 (03/29/06 06:10 PM)

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