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InvisibleHippie3
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Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags
    #4823969 - 10/19/05 08:56 AM (15 years, 10 months ago)

hi,
yer old friend Hippie here.
Just wanted to share something that's catching on over at mycotopia.net

a simple way to convert an empty syringe into an effective filter for air
to equalize pressure in sealed jars/bags
neutralizing the partial vacuum formed by sterilization
or the removal of fluid from a liquid culture jar.
see http://forums.mycotopia.net/showthread.php?t=5245
for the original thread info.
here's some pix-






ok,
that's how to make the airport itself, just pc it once to sterilize the interior
and it will filter air that passes thru it thereafter.


now how to use it on a jar of spore water or a liquid culture-
the lid is prepared thusly-









one can use this even to inject nutes/spores/air into sealed bottles of distilled water, sport drinks, etc. as needed.
many possible uses.
also see
http://forums.mycotopia.net/showthread.php?t=5177
and
http://forums.mycotopia.net/showthread.php?t=5001
and
http://forums.mycotopia.net/showthread.php?t=5212


here's how to use the airport to bubble air into a liquid culture to increase
dissolved gases and boost growth.






Quote:

I made a lid. i put 4 holes in it then put silicone over holes on both sides and allowed to dry for 24 hours. I then made my syringes i took a bunch of polyfil and stuffed it into 2 syringes and packed it down to make the filters then i took 1 syringe and siliconed an air tube into it to automat the process instead of blowing on one end(it makes it easier when bubbling the solution later) and i let it sit for 48 hours to totally let it dry it wasn't totally dry after 24 hours.

I started my LC by using a 250ml jar, i put 100ml water in the jar and 1 tablespoon of vanilla extract. i then put the lid on the jar seal side down b/c i want the jar to have a tight seal but left the ring loose on the jar. PC at 15 P.S.I. for 30 min then after PC i released the pressure from my cooker by the release valve and while hot i tightened the ring to keep seal. let cool. i then took a cotton swab with alcohol on it and put it over the hole and pierced it with one of the syringes to kill the vacuum in jar(or else it will suck all your spore syringe into the jar). i then used the same cotton swab and pierce through the silicone with my spore syringe. and allowed to grow. every 4 days i would stick my 2 syringes in to the holes. i put the non airtube syringe in first then the airtube one tipped the jar on its side and turned the pump on. the reason i like the air pump is that you can watch it bubble through the solution but also make sure you don't blow any into the other syringe.







i'm sure y'all can figure out where to go from here.
enjoy.
peace & love
-hip


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Edited by Hippie3 (10/19/05 02:20 PM)


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Invisibleohmatic
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4824029 - 10/19/05 09:49 AM (15 years, 10 months ago)

the pictures in this post do not work
and to view the pics @ mycotopia, one needs to be logged in.

fix your post - or its about useless.

btw, nice to see you still posting from time to time hip :smile:
peace ohm :mushroom2:


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: ohmatic]
    #4824036 - 10/19/05 09:52 AM (15 years, 10 months ago)

bummer,
the pic upload limits here make it hard to upload all at once but i'll try to
upload what i can directly
and do more later
sorry


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4824067 - 10/19/05 10:10 AM (15 years, 10 months ago)

here's the first in the series


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Edited by Hippie3 (10/19/05 10:18 AM)


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4824081 - 10/19/05 10:17 AM (15 years, 10 months ago)

ok,
here's the 2nd series-


and that's all i can upload now,
hit my daily limit,
sorry


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Edited by Hippie3 (10/19/05 10:19 AM)


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4824151 - 10/19/05 11:01 AM (15 years, 10 months ago)

hi
i just adjusted the permissions at mycotopia
should allow un-registered visitors to see the pix now,
can you ?


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InvisibleRoadkillM
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4824157 - 10/19/05 11:03 AM (15 years, 10 months ago)

Yes...I can see them now.

:smile:

Nice post hippie!~


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Roadkill]
    #4824362 - 10/19/05 12:14 PM (15 years, 10 months ago)

It can be used in 2 ways for introducing air/bubbling a LC. Hippie shows a air pump pumping through the polyfil. If you stuck another syringe in you could suck air out, drawing air in through the port, this could be with your mouth or a vacuum cleaner with some sort of attachment (may be a bit noisy!). If using your mouth I would prefer sucking air out, rather than blowing in through the filter. Also you could soak the syringe with polyfil in alcohol and store in a bag while not in use.

The real bonus I see is no need for a filter on your LC jar. So you have the best of both worlds. You can shake it well to aerate and introduce fresh air every few days and shake it again to aerate and split up the myc. Agar had a similar solution but it had tubes attached which was a bit more tricky to source and build.

I had a more complicated design here
http://www.shroomery.org/forums/showflat.php?Cat=0&Board=Forum2&Number=4235588

They can also be used for PFjars, you can fully silicone the innoc holes on the lid and screw it down tight and introduce fresh air every few days


Edited by blackout (10/19/05 12:19 PM)


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Invisiblemskip23
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Roadkill]
    #4824381 - 10/19/05 12:21 PM (15 years, 10 months ago)

Nice hip


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: mskip23]
    #4825182 - 10/19/05 03:51 PM (15 years, 10 months ago)

it works great hippie, thanks for the tek hippie. i am adjusting my syringe bottles to use the airport. nice pics (spore).

:peace:


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: backupwards]
    #4828509 - 10/20/05 07:40 AM (15 years, 10 months ago)

here's a link-
http://forums.mycotopia.net/showthread.php?p=194953
to a thread demonstrating
how to use the airport system
to turn a store-bought un-opened bottle of fruitwater
into a liquid culture,
in this case a 2 yr old syringe of mexA was used
wuth success,
now have some ~60 syringefuls that can by syphoned out for use
simply by inserting an airport
and an empty clean syringe in the silicon ports.






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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4828513 - 10/20/05 07:44 AM (15 years, 10 months ago)

btw
i'd request that
if you like this idea
plz bump up
my rating here,
some have trolled me in the past,
knocking it down.
i'd like my 5 shroom rating back, someday...
thx in advance.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4828546 - 10/20/05 08:37 AM (15 years, 10 months ago)

Very useful, Hippie3...thanks for sharing this.  :thumbup: :mushroom2:


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Holydiver]
    #4828565 - 10/20/05 09:11 AM (15 years, 10 months ago)

my pleasure,
i'd appreciate feedback from folks who try the airport system
in various applications
either posted here or at mycotopia.net.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4829291 - 10/20/05 02:14 PM (15 years, 10 months ago)





I have done something like that with LC's for a long time & it does work very well. I just use rubber tubing & rtv gasket sealer to seal the tubing into the lids.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar]
    #4829326 - 10/20/05 02:23 PM (15 years, 10 months ago)

indeed, i've seen many variants
but i figure almost everyone has a few empty syringes lying around.
soon i'll post more pix showing how to create bulk spore water jars
that can fill 20-30 syringes at a time,
using the airport system.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4829379 - 10/20/05 02:35 PM (15 years, 10 months ago)

Agars jars work well but if you have say 20 jars on the go then you need 20 caps to be built. Using filtered syringes you simply poke 2 holes in each cap and but a blob of silicone on them. Then use just the one filtered syringe. I put bleach in my syringe and squirt it through the filter before putting it in my jar.



Edited by blackout (10/20/05 02:36 PM)


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Invisibleagar
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4829432 - 10/20/05 02:45 PM (15 years, 10 months ago)



I just use one of these uncommon items (Socorex auto-loading syringe).
Slide the feed tube into a LC rubber tube, agitate jar & pump away.
Makes it EASY as eating pie, to inoculate 40 or 50 spawn bags, or jars - at one setting.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: blackout]
    #4829714 - 10/20/05 03:30 PM (15 years, 10 months ago)


Sun Tea jars (half & 1 gallon) can also be modified for LC's.
Simply alter the lid, remove spigot & replace with an injection port.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar]
    #4830177 - 10/20/05 05:28 PM (15 years, 10 months ago)

Showoffs :wink:

you guys are awesome! :smile:


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: blackout]
    #4830673 - 10/20/05 06:48 PM (15 years, 10 months ago)

Quote:

blackout said:
Agars jars work well but if you have say 20 jars on the go then you need 20 caps to be built. Using filtered syringes you simply poke 2 holes in each cap and but a blob of silicone on them. Then use just the one filtered syringe. I put bleach in my syringe and squirt it through the filter before putting it in my jar.







true enough,
hard to get much simpler/easier/cheaper
than stuffing an old syringe with polyfil,
poking a hole in a lid with an icepick
and applying a blob of silicon adhesive.
that's it,
do it for a penny a jar,
nothing permanent so jars can be stacked anywhere.
lay jar on side, upside down- matters not.
shake a LC until the tissue fragments,
no need for glass or magnets.
but one big advantage-
you can place the silicon on a plastic bottle
never opening it
and slide the needle in to inoculate.
you could inject apple juice into a gallon jug of distilled water,
right from the unopened jar of juice to the unopened jug of water
to create a nutrient solution
then inject a few spores-
voila! a heat-free flowhood-less way
to make a gallon of liquid inoculant at a time for mere pennies
in just a few minutes wih very few supplies needed.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4830830 - 10/20/05 07:11 PM (15 years, 10 months ago)

here;s another thread to check out-
http://forums.mycotopia.net/showthread.php?p=194915

one thing the airport system makes feasible
is 'canning' nutrient solution the old fashioned way-
lids with siliconed holes, rubber-side down
so it seals fully as it cools.
once cooled/sealed
inside the jar will be a partial vacuum.
if carefully inoculated to preserve most of the vacuum,
his experiment seems to indicate
that mycellia grows faster in the low pressure environment.
this is still bleeding edge stuff
with a need for more evidence/confirmation
but if the phenomenon proves real/consistent/significant
it could change how we do LC's,
dramatically speeding up the growth of tissue in liquids.
worth a few more tests, imho.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4832999 - 10/21/05 04:30 AM (15 years, 10 months ago)

Yes, I was talking to you about the apple juice at mycotpia. You can easily put a needle in the perforated hole on the tetra pack. Juice is usually 10-12% sugar so add 2 parts water to one juice, though weak LCs work fine too. Some sports drinks are now 3-5% sugars instead of the usual 10-12%. Lately I have been working in a/c bags as a cheap glove box. If you made a jar of 50/50 water/honey and heated it up it will be fluid enough to pour into a large bottle of water in a glovebox.

If working with vacuum just make sure you dont lose your whole syringe when you inject.

I do most of my sterilizing in another house, so transporting LCs used to be a big problem for me, it does solve it.

I have had great success adding large amounts of LC to grains and have some projects I will be working on soon that need a lot of LC.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar]
    #4833434 - 10/21/05 12:17 PM (15 years, 10 months ago)

Quote:

agar said:


I just use one of these uncommon items (Socorex auto-loading syringe).
Slide the feed tube into a LC rubber tube, agitate jar & pump away.
Makes it EASY as eating pie, to inoculate 40 or 50 spawn bags, or jars - at one setting.




where did you get that gun???!!!


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4836334 - 10/21/05 11:37 PM (15 years, 10 months ago)

eBay for $26 plus S/H. They run arounf $140 retail & depending on what fittings you get with it. Soccorex is the best in the world.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4844780 - 10/24/05 08:52 AM (15 years, 10 months ago)

a couple more pix
showing airport in action
bubbling air into a liquid culture [karo jar]



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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4844796 - 10/24/05 09:17 AM (15 years, 10 months ago)

thats a pretty dark karo jar... did you use malt extract in that at all hip?


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4844849 - 10/24/05 10:09 AM (15 years, 10 months ago)

no,
but instead
vanilla extract was added.
for some info on vanilla
see
laz's vanilla tek.


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Edited by Hippie3 (10/24/05 10:23 AM)


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OfflineTweexican
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4896283 - 11/05/05 03:38 AM (15 years, 10 months ago)

In the picture with the airport being used to pull sterile distilled water... was the water used to make syringes or straight for liquid culture use???


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4896597 - 11/05/05 07:01 AM (15 years, 10 months ago)

it was used to make syringes in that particular case.
the bottle of fruitwater was used as a liquid culture [mexicana A]


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4897126 - 11/05/05 01:29 PM (15 years, 10 months ago)

so was it, pull water from bottle, and hydrate spores with a brand new syringe?

if so, how was the contam turn out?


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4897153 - 11/05/05 01:38 PM (15 years, 10 months ago)

correct.
no contams.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4897716 - 11/05/05 05:37 PM (15 years, 10 months ago)

how exactly was the transfer of spores to the syringe done?

shot glass tek?


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4897755 - 11/05/05 05:48 PM (15 years, 10 months ago)

no,
the print was placed inside small ziplock bag
and water was injected, stirred to mix then sucked back into syringe

see
http://forums.mycotopia.net/showthread.php?t=5782


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5404193 - 03/15/06 05:45 PM (15 years, 6 months ago)

a new experiment using
the airport instead of a
dry verm. barrier in cake culture
see
http://forums.mycotopia.net/showthread.php?p=231866


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5411154 - 03/17/06 03:47 AM (15 years, 6 months ago)

This thread needs thumbnails. :smirk:


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Dragonaut]
    #5428746 - 03/22/06 07:58 AM (15 years, 5 months ago)

ask
and ye shall receive.




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OfflineJSshroom
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5437878 - 03/24/06 12:56 PM (15 years, 5 months ago)

are the spore bags being sterilized in anyway before puttig the spores in and conpleting the sealing process.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
    #5439087 - 03/24/06 07:54 PM (15 years, 5 months ago)

nope
sterile enough from the factory
although one could swab with rubbing alky if desired


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
    #5440389 - 03/25/06 08:57 AM (15 years, 5 months ago)

permanent endless supply of sterlie air
sometimes one needs a source of sterile air.
here's a simple way.
quart jar fitted with twin silicon ported lid
about 1/2 inch of water in bottom
run the pc to sterilize insides
then place airport in one hole to equalize pressure.
empty syringe inserted thru other hole
can then be filled with sterile air as needed.
the polyfil syringe will filter replacement air
maintaining equal pressure







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Edited by Hippie3 (03/29/06 08:10 PM)


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5440401 - 03/25/06 09:26 AM (15 years, 5 months ago)

Or one can just put the needle tip in a flame and pull back the plunger.


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Pinback]
    #5440493 - 03/25/06 11:09 AM (15 years, 5 months ago)

i sure wouldn't rely on that.
flame doesn't instantly kill every microbe sucked thru it.
try that and shoot the air on some agar...
also flame is inappropriate for some situations,
such as a lysol-laden glovebox.


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Edited by Hippie3 (03/25/06 12:08 PM)


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5440638 - 03/25/06 12:56 PM (15 years, 5 months ago)

Good idea hippie, but what is the point of the water in there?


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Jaeger]
    #5443411 - 03/26/06 09:32 AM (15 years, 5 months ago)

steam is far more effective than dry heat.
pc it, steam kills everything inside.
baking it hot enough to do that
would ruin the lid's rubber seal.
proly just a spoonful inside is plenty.


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OfflineTweexican
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5456373 - 03/29/06 08:42 PM (15 years, 5 months ago)

this seems like a lot of trouble.

i liked what you did with the store bought flavored water for lc use. ingenius.

what use does an airport serve if you have to go to so much trouble just to keep up with the maintenence of oxygenating your jars?

in this case instead of trying to get "sterile air", wouldn't it be easier to just use a polyfil filter for your jars?


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #5458249 - 03/30/06 07:17 AM (15 years, 5 months ago)

it's no great trouble to squeeze out a blob of silicon or two.
it's no trouble to suck air in a syringe.
i'm not trying to sell you an air kit,
if you don't want to try it,
don't.
someone somewhere will,
and that is good enough reason
to post it.
see this thread for current example of
trouble some folks have with polyfil-
wet polyfil
one last thought-
the easy way isn't always best.
polyfil can get wet and let contams thru.
esp. when doing liquid cultures.
this air supply allows one to give LCs a hard shake [try that with a polyfil filtered lid] to break up mycellia, inject sterile air.
one LC can make many syringefuls of inoculant,
making the 'trouble' rather worthwhile.
some folks have trouble finding polyfil,
maybe they live in thailand in a hut on edge of jungle
with no walmart in sight.
maybe they just want to experiment with
something new to see if it can be done.


Edited by Hippie3 (03/30/06 07:43 AM)


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #5458262 - 03/30/06 07:27 AM (15 years, 5 months ago)

Quote:

Tweexican said:

i liked what you did with the store bought flavored water for lc use. ingenius.





speaking of which-
that culture is now 6 months old
and still doing well,
just filled another syringe of mexA tissue a few days ago.
growth has never gotten dense
but there's only a 2% sugars content.
drawing the LC out of the lower airport works like a charm
as gravity settles the tissue to the bottom
so you can suck it right into the needle,
air bubbles in the upper airport to compensate the volume lost.
i'm seriously thinking about
trying to 'feed' this LC by
injecting a little fresh karo water.


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OfflineJSshroom
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5500408 - 04/10/06 06:34 PM (15 years, 5 months ago)

Heres an airport I made.


Its an air valve from the plumbing section that was a hell of a tight fit but threaded onto a polyfilled syringe so that the airpump plugs right into it.

I Pc my airports everytime I use them is this necessary?


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
    #5500416 - 04/10/06 06:37 PM (15 years, 5 months ago)

no, i just re=pc them after a few uses.


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