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InvisibleHippie3
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Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags * 1
    #4823969 - 10/19/05 06:56 AM (18 years, 3 months ago)

hi,
yer old friend Hippie here.
Just wanted to share something that's catching on over at mycotopia.net

a simple way to convert an empty syringe into an effective filter for air
to equalize pressure in sealed jars/bags
neutralizing the partial vacuum formed by sterilization
or the removal of fluid from a liquid culture jar.
see http://forums.mycotopia.net/showthread.php?t=5245
for the original thread info.
here's some pix-






ok,
that's how to make the airport itself, just pc it once to sterilize the interior
and it will filter air that passes thru it thereafter.


now how to use it on a jar of spore water or a liquid culture-
the lid is prepared thusly-









one can use this even to inject nutes/spores/air into sealed bottles of distilled water, sport drinks, etc. as needed.
many possible uses.
also see
http://forums.mycotopia.net/showthread.php?t=5177
and
http://forums.mycotopia.net/showthread.php?t=5001
and
http://forums.mycotopia.net/showthread.php?t=5212


here's how to use the airport to bubble air into a liquid culture to increase
dissolved gases and boost growth.






Quote:

I made a lid. i put 4 holes in it then put silicone over holes on both sides and allowed to dry for 24 hours. I then made my syringes i took a bunch of polyfil and stuffed it into 2 syringes and packed it down to make the filters then i took 1 syringe and siliconed an air tube into it to automat the process instead of blowing on one end(it makes it easier when bubbling the solution later) and i let it sit for 48 hours to totally let it dry it wasn't totally dry after 24 hours.

I started my LC by using a 250ml jar, i put 100ml water in the jar and 1 tablespoon of vanilla extract. i then put the lid on the jar seal side down b/c i want the jar to have a tight seal but left the ring loose on the jar. PC at 15 P.S.I. for 30 min then after PC i released the pressure from my cooker by the release valve and while hot i tightened the ring to keep seal. let cool. i then took a cotton swab with alcohol on it and put it over the hole and pierced it with one of the syringes to kill the vacuum in jar(or else it will suck all your spore syringe into the jar). i then used the same cotton swab and pierce through the silicone with my spore syringe. and allowed to grow. every 4 days i would stick my 2 syringes in to the holes. i put the non airtube syringe in first then the airtube one tipped the jar on its side and turned the pump on. the reason i like the air pump is that you can watch it bubble through the solution but also make sure you don't blow any into the other syringe.







i'm sure y'all can figure out where to go from here.
enjoy.
peace & love
-hip


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Edited by Hippie3 (10/19/05 12:20 PM)


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: ohmatic] * 1
    #4824036 - 10/19/05 07:52 AM (18 years, 3 months ago)

bummer,
the pic upload limits here make it hard to upload all at once but i'll try to
upload what i can directly
and do more later
sorry


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4824067 - 10/19/05 08:10 AM (18 years, 3 months ago)

here's the first in the series


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Edited by Hippie3 (10/19/05 08:18 AM)


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4824081 - 10/19/05 08:17 AM (18 years, 3 months ago)

ok,
here's the 2nd series-


and that's all i can upload now,
hit my daily limit,
sorry


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Edited by Hippie3 (10/19/05 08:19 AM)


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 1
    #4824151 - 10/19/05 09:01 AM (18 years, 3 months ago)

hi
i just adjusted the permissions at mycotopia
should allow un-registered visitors to see the pix now,
can you ?


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: backupwards] * 1
    #4828509 - 10/20/05 05:40 AM (18 years, 3 months ago)

here's a link-
http://forums.mycotopia.net/showthread.php?p=194953
to a thread demonstrating
how to use the airport system
to turn a store-bought un-opened bottle of fruitwater
into a liquid culture,
in this case a 2 yr old syringe of mexA was used
wuth success,
now have some ~60 syringefuls that can by syphoned out for use
simply by inserting an airport
and an empty clean syringe in the silicon ports.






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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3] * 2
    #4828513 - 10/20/05 05:44 AM (18 years, 3 months ago)

btw
i'd request that
if you like this idea
plz bump up
my rating here,
some have trolled me in the past,
knocking it down.
i'd like my 5 shroom rating back, someday...
thx in advance.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Holydiver] * 1
    #4828565 - 10/20/05 07:11 AM (18 years, 3 months ago)

my pleasure,
i'd appreciate feedback from folks who try the airport system
in various applications
either posted here or at mycotopia.net.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: agar] * 1
    #4829326 - 10/20/05 12:23 PM (18 years, 3 months ago)

indeed, i've seen many variants
but i figure almost everyone has a few empty syringes lying around.
soon i'll post more pix showing how to create bulk spore water jars
that can fill 20-30 syringes at a time,
using the airport system.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: blackout]
    #4830673 - 10/20/05 04:48 PM (18 years, 3 months ago)

Quote:

blackout said:
Agars jars work well but if you have say 20 jars on the go then you need 20 caps to be built. Using filtered syringes you simply poke 2 holes in each cap and but a blob of silicone on them. Then use just the one filtered syringe. I put bleach in my syringe and squirt it through the filter before putting it in my jar.







true enough,
hard to get much simpler/easier/cheaper
than stuffing an old syringe with polyfil,
poking a hole in a lid with an icepick
and applying a blob of silicon adhesive.
that's it,
do it for a penny a jar,
nothing permanent so jars can be stacked anywhere.
lay jar on side, upside down- matters not.
shake a LC until the tissue fragments,
no need for glass or magnets.
but one big advantage-
you can place the silicon on a plastic bottle
never opening it
and slide the needle in to inoculate.
you could inject apple juice into a gallon jug of distilled water,
right from the unopened jar of juice to the unopened jug of water
to create a nutrient solution
then inject a few spores-
voila! a heat-free flowhood-less way
to make a gallon of liquid inoculant at a time for mere pennies
in just a few minutes wih very few supplies needed.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4830830 - 10/20/05 05:11 PM (18 years, 3 months ago)

here;s another thread to check out-
http://forums.mycotopia.net/showthread.php?p=194915

one thing the airport system makes feasible
is 'canning' nutrient solution the old fashioned way-
lids with siliconed holes, rubber-side down
so it seals fully as it cools.
once cooled/sealed
inside the jar will be a partial vacuum.
if carefully inoculated to preserve most of the vacuum,
his experiment seems to indicate
that mycellia grows faster in the low pressure environment.
this is still bleeding edge stuff
with a need for more evidence/confirmation
but if the phenomenon proves real/consistent/significant
it could change how we do LC's,
dramatically speeding up the growth of tissue in liquids.
worth a few more tests, imho.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #4844780 - 10/24/05 06:52 AM (18 years, 3 months ago)

a couple more pix
showing airport in action
bubbling air into a liquid culture [karo jar]



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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4844849 - 10/24/05 08:09 AM (18 years, 3 months ago)

no,
but instead
vanilla extract was added.
for some info on vanilla
see
laz's vanilla tek.


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Edited by Hippie3 (10/24/05 08:23 AM)


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4896597 - 11/05/05 05:01 AM (18 years, 2 months ago)

it was used to make syringes in that particular case.
the bottle of fruitwater was used as a liquid culture [mexicana A]


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4897153 - 11/05/05 11:38 AM (18 years, 2 months ago)

correct.
no contams.


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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Tweexican]
    #4897755 - 11/05/05 03:48 PM (18 years, 2 months ago)

no,
the print was placed inside small ziplock bag
and water was injected, stirred to mix then sucked back into syringe

see
http://forums.mycotopia.net/showthread.php?t=5782


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Hippie3]
    #5404193 - 03/15/06 03:45 PM (17 years, 10 months ago)

a new experiment using
the airport instead of a
dry verm. barrier in cake culture
see
http://forums.mycotopia.net/showthread.php?p=231866


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: Dragonaut]
    #5428746 - 03/22/06 05:58 AM (17 years, 10 months ago)

ask
and ye shall receive.




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InvisibleHippie3
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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
    #5439087 - 03/24/06 05:54 PM (17 years, 10 months ago)

nope
sterile enough from the factory
although one could swab with rubbing alky if desired


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Re: Airports- A Simple Way to Inject/Extract Liquids/Gases From Sealed Jars/Bags [Re: JSshroom]
    #5440389 - 03/25/06 06:57 AM (17 years, 10 months ago)

permanent endless supply of sterlie air
sometimes one needs a source of sterile air.
here's a simple way.
quart jar fitted with twin silicon ported lid
about 1/2 inch of water in bottom
run the pc to sterilize insides
then place airport in one hole to equalize pressure.
empty syringe inserted thru other hole
can then be filled with sterile air as needed.
the polyfil syringe will filter replacement air
maintaining equal pressure







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Edited by Hippie3 (03/29/06 06:10 PM)


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