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OfflineHammerloaf
addict

Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 20 years, 7 months
Slow Show
    #480455 - 12/05/01 06:38 AM (22 years, 3 months ago)

How long would one have to wait for honey water to start to show signs of colonization, in low temp envrionment?

anyone have a ball park estimate for me? :)

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Offlinelemming
evil

Registered: 10/28/01
Posts: 107
Last seen: 18 years, 7 months
Re: Slow Show [Re: Hammerloaf]
    #480463 - 12/05/01 07:06 AM (22 years, 3 months ago)

2-3 days in good conditions, so I guess 4-5 if you're not keeping it at optimal temperature.


--------------------
As evil as a dwarf can get...

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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: Slow Show [Re: Hammerloaf]
    #480483 - 12/05/01 07:36 AM (22 years, 3 months ago)

From spores, about a week at room temp.

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OfflineHammerloaf
addict

Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 20 years, 7 months
Re: Slow Show [Re: puscle]
    #480490 - 12/05/01 07:48 AM (22 years, 3 months ago)

dammit puscle! I said honey water! :)

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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: Slow Show [Re: Hammerloaf]
    #480539 - 12/05/01 09:13 AM (22 years, 3 months ago)

What are you using to inoculate the honey water? Spores? With a piece of mycelia it'll go quicker.

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OfflineHammerloaf
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Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 20 years, 7 months
Re: Slow Show [Re: puscle]
    #480540 - 12/05/01 09:16 AM (22 years, 3 months ago)

A friend used the last cc of spore solution he recieved from THC.. I realize that a piece of mycelia would be better.. but my friend has none right now other then on his cakes.. would a pin work? what is the best part of a living shroom to use? I've heard of coreing it..

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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: Slow Show [Re: Hammerloaf]
    #480543 - 12/05/01 09:22 AM (22 years, 3 months ago)

It gets trickier, contam-wise when using mycelia or a piece of shroom. But yes, a piece from inside the stem given a one minute bath in peroxide will work. This is cloning, you need to be super clean, as in glovebox clean.

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OfflineHammerloaf
addict

Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 20 years, 7 months
Re: Slow Show [Re: puscle]
    #480544 - 12/05/01 09:23 AM (22 years, 3 months ago)

thats cool.. I use a glove box anyhow..

whats the best method for coring the stem?

someone mentioned a syringe might work well.. I dunno about that.. but a sterile small metal tube seems like it might do the trick..

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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: Slow Show [Re: Hammerloaf]
    #480552 - 12/05/01 09:27 AM (22 years, 3 months ago)

I've always used an exacto knife, but I like your idea.

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OfflineHammerloaf
addict

Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 20 years, 7 months
Re: Slow Show [Re: puscle]
    #480553 - 12/05/01 09:30 AM (22 years, 3 months ago)

*GASP*

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