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OfflineHammerloaf
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Registered: 11/26/01
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Slow Show
    #480455 - 12/05/01 08:38 AM (16 years, 4 months ago)

How long would one have to wait for honey water to start to show signs of colonization, in low temp envrionment?

anyone have a ball park estimate for me? :)


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Offlinelemming
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Registered: 10/28/01
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Re: Slow Show [Re: Hammerloaf]
    #480463 - 12/05/01 09:06 AM (16 years, 4 months ago)

2-3 days in good conditions, so I guess 4-5 if you're not keeping it at optimal temperature.


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Invisiblepuscle
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Registered: 01/07/01
Posts: 4,539
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Re: Slow Show [Re: Hammerloaf]
    #480483 - 12/05/01 09:36 AM (16 years, 4 months ago)

From spores, about a week at room temp.


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OfflineHammerloaf
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Re: Slow Show [Re: puscle]
    #480490 - 12/05/01 09:48 AM (16 years, 4 months ago)

dammit puscle! I said honey water! :)


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Invisiblepuscle
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Re: Slow Show [Re: Hammerloaf]
    #480539 - 12/05/01 11:13 AM (16 years, 4 months ago)

What are you using to inoculate the honey water? Spores? With a piece of mycelia it'll go quicker.


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OfflineHammerloaf
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Re: Slow Show [Re: puscle]
    #480540 - 12/05/01 11:16 AM (16 years, 4 months ago)

A friend used the last cc of spore solution he recieved from THC.. I realize that a piece of mycelia would be better.. but my friend has none right now other then on his cakes.. would a pin work? what is the best part of a living shroom to use? I've heard of coreing it..


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Invisiblepuscle
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Re: Slow Show [Re: Hammerloaf]
    #480543 - 12/05/01 11:22 AM (16 years, 4 months ago)

It gets trickier, contam-wise when using mycelia or a piece of shroom. But yes, a piece from inside the stem given a one minute bath in peroxide will work. This is cloning, you need to be super clean, as in glovebox clean.


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OfflineHammerloaf
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Re: Slow Show [Re: puscle]
    #480544 - 12/05/01 11:23 AM (16 years, 4 months ago)

thats cool.. I use a glove box anyhow..

whats the best method for coring the stem?

someone mentioned a syringe might work well.. I dunno about that.. but a sterile small metal tube seems like it might do the trick..


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Invisiblepuscle
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Registered: 01/07/01
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Re: Slow Show [Re: Hammerloaf]
    #480552 - 12/05/01 11:27 AM (16 years, 4 months ago)

I've always used an exacto knife, but I like your idea.


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OfflineHammerloaf
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Re: Slow Show [Re: puscle]
    #480553 - 12/05/01 11:30 AM (16 years, 4 months ago)

*GASP*


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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