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Offlinejanelle1502
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Registered: 10/30/04
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Do I have to make a spore print?
    #4779200 - 10/09/05 09:45 PM (19 years, 3 months ago)

or can I make one from a little slice of fresh mushroom tissue? That would save me a lot of time. Every tek I read on LC doesn't mention inoculating the malt solution with anything but spore solution...


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Invisiblejarroddupont
I shroom we Allshroom

Registered: 10/02/05
Posts: 2,106
Loc: midwest of ...
Re: Do I have to make a spore print? [Re: janelle1502]
    #4779222 - 10/09/05 09:48 PM (19 years, 3 months ago)

from my reading, i only recall using tissues on agar...I may be wrong.......


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InvisibleJackTackle
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Registered: 09/18/05
Posts: 282
Loc: behind you
Re: Do I have to make a spore print? [Re: janelle1502]
    #4779228 - 10/09/05 09:49 PM (19 years, 3 months ago)

if you make a print tonight it will be ready when you wake up in the morning
what's the hurry?


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Offlinejanelle1502
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Re: Do I have to make a spore print? [Re: janelle1502]
    #4779245 - 10/09/05 09:52 PM (19 years, 3 months ago)

Maybe I'm just lazy :wink:


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OfflineA0999
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Re: Do I have to make a spore print? [Re: janelle1502]
    #4779384 - 10/09/05 10:11 PM (19 years, 3 months ago)

look into cloning. check out anno's site. fungifun.com of something like that.

but, i would recomend a liquid culture...


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OfflineCryogenicz
what?
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Registered: 07/01/04
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Re: Do I have to make a spore print? [Re: A0999]
    #4779932 - 10/09/05 11:24 PM (19 years, 3 months ago)

The nice thing about spore prints- is that you can make a shit load of them. If you have some contams you can just use another print. The other good thing about it is that in a year from now you can take a print and grow from it. With a dish- they are perishable. I have used syringes as old as 2.5 years old.


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Offline_OttO_
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Re: Do I have to make a spore print? [Re: janelle1502]
    #4780087 - 10/09/05 11:45 PM (19 years, 3 months ago)

Quote:

janelle1502 said:
or can I make one from a little slice of fresh mushroom tissue? That would save me a lot of time. Every tek I read on LC doesn't mention inoculating the malt solution with anything but spore solution...




Yes you can make a LC out of fresh mushroom tissue - this is called cloning. Cut a peice of tissue from the centre of a stem with a sterile knife and drop it in to your LC in sterile conditions.

Alternatively, you can take a spore print and start a LC with that - instead of mushroom tissue, just use a sterile knife to scrape a few spores into your LC in sterile conditions. This is called multispore innocluation.

Take a few prints and store them in little plastic seal bags and they will last for many years. I have had no problem starting a LC with a three year old print.

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