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Invisiblewise_gardener
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Registered: 08/02/05
Posts: 157
How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ?
    #4771595 - 10/08/05 01:00 AM (19 years, 3 months ago)

Hi,
I have trouble to figure how to transfer the cap from the fruiting chamber to the glovebox whiteout contams them. I can keep a relative sterility inside the fruiting chamber using a 3m furnace filter in the air filtration, and the glovebox can be sterilized with lysol/bleach solution.. But the air between the 2 box still open to contams! Any idea how to keep the transfer sterile ? Thanks

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OfflineKotton
Manic Hispanic

Registered: 09/11/05
Posts: 120
Last seen: 19 years, 2 months
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: wise_gardener]
    #4771631 - 10/08/05 01:08 AM (19 years, 3 months ago)

1. Get thumb Tack and poke the top of the cap of the one you want to print.

2. Get your jar you sterilized in a PC with foil on the bottom of the jar.

3. Spray area with oust. Cut the mushroom you placed the thumb tack on near the cap, open your sterile jar quickly and insert the cap on the foil close the jar up tightly.

4. After a day You can open up your jar in your glove box and proceed with making a syringe. You can pull the mushroom out of the jar with the thumb tack so its easy to grab.


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:scaryshroom:  :lovemeds::scaryshroom:

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Invisiblejarroddupont
I shroom we Allshroom

Registered: 10/02/05
Posts: 2,106
Loc: midwest of ...
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: wise_gardener]
    #4771638 - 10/08/05 01:09 AM (19 years, 3 months ago)

I was told by an expert how he makes his syringes (assuming that's what you're doing with your prints) he puts his caps in the bottom of a jar to print and adds sterile water to the jars (after spores have dropped and cap removed)and sucks them into his syringe, point being you could tear off the cap from your FC and place into a jar without leaving your FC...just an idea...


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P. Cubensis Growth Parameters
"All mushrooms are edible, but some only once."
-- Croatian Proverb

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OfflineKotton
Manic Hispanic

Registered: 09/11/05
Posts: 120
Last seen: 19 years, 2 months
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: jarroddupont]
    #4771668 - 10/08/05 01:14 AM (19 years, 3 months ago)

Yes basically thats right.


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:scaryshroom:  :lovemeds::scaryshroom:

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InvisibleAndroctonus
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Registered: 09/17/05
Posts: 1,479
Loc: L.I.S.
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: wise_gardener]
    #4771799 - 10/08/05 01:49 AM (19 years, 3 months ago)

Or take a look at this tek.


http://www.shroomery.org/index/par/7923

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OfflinePookztA
Medical Student

Registered: 09/09/05
Posts: 1,225
Loc: Illinois
Last seen: 13 years, 3 months
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: Androctonus]
    #4771810 - 10/08/05 01:52 AM (19 years, 3 months ago)

man thats a good idea, i see all these teks for making a print, then scraping print spores into a jar with sterile water, then making the syringe. it seems much quicker, more contam free, and lots easier to simply place it in a jar, let hte spores drop, add sterile water and just suck it up. u dont even really have to make a print if u think about it haha, drop dem spores, add water, and enjoy :smile:


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Abrahm
Spreading Psytrance & Love in the Midwest USA

Expand Your Consciousness. :mushroomgrow:

:peace: 9/11 Challenge: Explain the Evidence http://pookzta.blogspot.com

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InvisibleAndroctonus
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Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: PookztA]
    #4772545 - 10/08/05 10:53 AM (19 years, 3 months ago)

Yea I really liked this idea. You only need to draw up what you need.


--------------------
The red grass, up to my knees
An aura comes towards me

Someone's changed the formula
Un-chaining another law

The structures, the colors
Uniform chaos is alternating

The nature of things

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OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
Loc: I love that spore drop
Last seen: 1 year, 2 months
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: Androctonus]
    #4772653 - 10/08/05 11:26 AM (19 years, 3 months ago)

the only problem is if it does contam..all the syringes are toast


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First Grow No more carpet tek

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Invisiblewise_gardener
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Male
Registered: 08/02/05
Posts: 157
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: JSshroom]
    #4772930 - 10/08/05 01:07 PM (19 years, 3 months ago)

Thanks for all,
I already have couple of idea but want you to share your experience :wink: I like the Kotton/jarroddupont concept. I'll stop all the air circulation in the fruiting chamber 4 to 5 hour before veil break. This will let all the possible contams drop down before spore exposure. I'll sterilize the air room with a 10% bleach solution before opening the FC lid too!
I'm kinda about to be self sufficient!  :cool:

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InvisibleFooMan
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Posts: 8,957
Loc: Earth Flag
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Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: PookztA]
    #4772971 - 10/08/05 01:20 PM (19 years, 3 months ago)



I do it all the time. Don't forget to swap the metal lid out for a tyvek lid before PC'ing. The lid allows air exchange so that you don't get condensation in the jar while you are waiting for the cap to drop spores.

Magash was the person that I originally saw posting this tek with foil on the bottom of the jar. I just use the jar by itself to make things as simple as possible. :thumbup:


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Quick WBS Prep

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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: FooMan]
    #4773103 - 10/08/05 02:22 PM (19 years, 3 months ago)

I don't know if this is recomended but I don't PC my jars I just wipe them down with 99% isopropanol alcohol. Found it at walmart. I don't PC used syringes either just fill them with boiling water and let it sit for two mins then squirt it out and repeat.


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My Humidifier Reservoir Expansion Tek

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Btw- You are not what you own!

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InvisibleGnuBobo
Frilly Cuffs Extraordinaire
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Registered: 06/17/04
Posts: 43,754
Loc: Charisma
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: musher_420]
    #4773661 - 10/08/05 04:57 PM (19 years, 3 months ago)

I pc a couple of folded pieces of tin foil, then slice the cap off with a pc'd exacto, put it inside one of the folded sterile tin foil pieces, transfer that to my glovebox. Oust my glovebox, then use the 9er tek with a wire "x" suspended over a sterilized jar of water...transfer the cap to the wire, cover with a piece of cloth I sterilzed with the other stuff, let it sit in the glovebox 24 hours, then put a lid with a small silicone blob on (for drawing syringes through). Leaves me with around 100-200ml of sterile good-density spore water. That expands to god knows how much LC....


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!

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OfflinePookztA
Medical Student

Registered: 09/09/05
Posts: 1,225
Loc: Illinois
Last seen: 13 years, 3 months
Re: How to transfer shroom cap from fruiting chamber to glovebox for print whitout contams ? [Re: musher_420]
    #4780545 - 10/10/05 01:43 AM (19 years, 3 months ago)

Quote:

musher_420 said:
I don't know if this is recomended but I don't PC my jars I just wipe them down with 99% isopropanol alcohol.  Found it at walmart.  I don't PC used syringes either just fill them with boiling water and let it sit for two mins then squirt it out and repeat.




that should be fine, i havent had any problems with a combo of lysol / alcohol yet, and i plan on sterilizing my syringes using the "boil water and suck it up into the syringe, and squirt it out" method as well, so PCing isnt necessary, but if you have a PC, why not? i dont own one, and dont plan on getting one for a while... :smile:


--------------------
Abrahm
Spreading Psytrance & Love in the Midwest USA

Expand Your Consciousness. :mushroomgrow:

:peace: 9/11 Challenge: Explain the Evidence http://pookzta.blogspot.com

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