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Offlinediablovt1125
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Frist Time Grower...First Signs of Life!!!
    #4751284 - 10/03/05 10:01 PM (11 years, 2 months ago)

Hello again everyone, let me start by saying that this is my first grow and I am following the PF TEK.  Its been exactly a week since inoculation and heres my results so far.

These Jars are B+ and appear to be coming along excellently.  No signs of contaminants, lots of growth popping up everywhere.





These six jars are Cambodian strain cubensis.  The main reason I'm posting this is because I am very confused as to what is going on in these jars.  I'm worried they are contaminated!? 







Let me Clarify that the area inside the red circles is the area where growth (or whatever that is) can be seen.  The stuff outside the red circle is simply the substrate.  I realized the images above can be looked at both ways so I just wanted to point that out.  It looks almost as if the area where the spore solution was put in is abnormally wet or something.



Anyone with experience with Cambodians or just this whole process in general, any help would be great here.  If they're Contaminated I want to get them out of the incubator soon.  Whats weird though is that all the B+ jars are doing great, and all the Cam jars are...well I'm not sure.  Thanks again for the help and you'
ll be hearing from me from time to time with questions for the next few weeks.  I appreciate the help  :grin:


Edited by diablovt1125 (10/03/05 10:44 PM)


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Offlinedjred
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Re: Frist Time Grower...First Signs of Life!!! [Re: diablovt1125]
    #4751308 - 10/03/05 10:05 PM (11 years, 2 months ago)

cambo's are fucking amazing..not to visual unless you do a fat dose.good energy from them fuckers im going round 2 i was impressed but i did bulk grow cow shit straw so i don't know if brf jars r going to yield the same pontency


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Invisiblemskip23
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Re: Frist Time Grower...First Signs of Life!!! [Re: djred]
    #4751383 - 10/03/05 10:17 PM (11 years, 2 months ago)

deff contam!!


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url=https://files.shroomery.org/files/05-26/988356075-Picture-278.gif][/url


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Invisiblerod
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Re: Frist Time Grower...First Signs of Life!!! [Re: diablovt1125]
    #4751427 - 10/03/05 10:25 PM (11 years, 2 months ago)

did the B+, and Cam,s come from the same supplier? The B+ look normal,
but something is going on with the Cam,s.Did you follow the same tek
for both?


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Offlinediablovt1125
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Re: Frist Time Grower...First Signs of Life!!! [Re: rod]
    #4751486 - 10/03/05 10:39 PM (11 years, 2 months ago)

They both came from Spore's Direct and I am following the same TEK (PF TEK) for both...


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InvisibleTurkish
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Re: Frist Time Grower...First Signs of Life!!! [Re: diablovt1125]
    #4752136 - 10/04/05 12:54 AM (11 years, 2 months ago)

That definitely looks like contamination. Are the growth areas inside your Cambodian jars consistent with your inoculation points?


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OfflinePookztA
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Re: Frist Time Grower...First Signs of Life!!! [Re: Turkish]
    #4752361 - 10/04/05 01:37 AM (11 years, 2 months ago)

uh contam? i have never seen a jar colonized with any cubensis strain that didnt generate brilliant, white, mycelium... that shit is darker in color than the actual substrate lol, is it your camera? or did you not use absolutely sterile conditions my friend?


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Offlinediablovt1125
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Re: Frist Time Grower...First Signs of Life!!! [Re: PookztA]
    #4752585 - 10/04/05 02:14 AM (11 years, 2 months ago)

I believe it was something to do with the syringe it's self. They were inoculated in the same setting as the other 6 jars of B+... I'm not sure what exactly happened, I guess Ill just have to start anew with those jars.


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Know yourself, know your substance, and know your source


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Offlinekurupt
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Re: Frist Time Grower...First Signs of Life!!! [Re: diablovt1125]
    #4752891 - 10/04/05 04:24 AM (11 years, 2 months ago)

damn those looks pretty nastey contam...


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Offlineoniq
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Re: Frist Time Grower...First Signs of Life!!! [Re: kurupt]
    #4752902 - 10/04/05 04:31 AM (11 years, 2 months ago)

Your substrate looks overly wet, did you PC? Cover up holes while PCing?


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InvisibleLand_Crab
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Re: Frist Time Grower...First Signs of Life!!! [Re: diablovt1125]
    #4752991 - 10/04/05 06:42 AM (11 years, 2 months ago)

Well the contamination either came from the substrate, the environment, your body, or the syringe. You probably already know most of these, but here are a few tips anyway:

- Spray down the area you are inoculating in with lysol (it should be reasonably clean to begin with), and do NOT inoculate in an area with free air circulation, particularly from the outside.
- When you spooned the substrate into the jars, did you make sure to wipe away any excess substrate that might have been sticking to the (top) of the inside of the jars? Excess substrate there can guide contaminates in and down. (Use rubbing alcohol to wipe clean.)
- To be extra-careful, take a shower before you inoculate. If you haven't had one recently, you can rightly assume that you are covered in contaminants. At the very least, wash your hands and wrists thoroughly with anti-bacterial soap, then rub down your hands and fingers with rubbing alcohol.
- You flame-sterilized the needle, of course..? After the needle has been heated sufficiently, cool it by wrapping a paper towel soaked in rubbing alcohol around it. -This will also help sterilize (and clean excess carbon if you're using a candle).
- Try to disturb the top layer of vermiculite as little as possible when inoculating.
- When handling the jars, move slowly and steadily.

Good luck!


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Offlinediablovt1125
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Re: Frist Time Grower...First Signs of Life!!! [Re: Land_Crab]
    #4754488 - 10/04/05 03:39 PM (11 years, 2 months ago)

Thanks again everyone...I will be re-innoculating the jars this afternoon, Im hoping for better luck!


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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