Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags

Jump to first unread post Pages: | 1 | 2 | 3  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: Nitrogen as key to potency [Re: Zen Peddler]
    #4685977 -

As has been discussed above, the "nitrogen content" (at least, in any basic sense) of the substrate is a misleading number. Excluding water, nearly every molecule which constitutes the fungus contains vastly more nitrogen than a psilocybin molecule does. Even speaking only of proteins, which make up 30% (ish) of the mass, each protein molecule contains several thousand times more nitrogen than a psilocybin molecule.

This is the whole point of this thread. Nitrogen content alone has no impact on alkaloid production. The question, as stated several times above, is what form the nitrogen must be in in order TO have an impact.

As I've explained in other threads, there's little reason to think that trytophan precursors will get you very far in increased alkaloid content, because tryptophan decarboxylase is inhibited by tryptamine, psilocybin and psilocin. As a result, there's little hope for coercing the production of large quantities of alkaloids by increasing tryptophan levels over those which naturally occur.

Needless to say, none of this is true of compounds which metabolically occur after tryptophan.

Extras: Filter Print Post Top
Re: Nitrogen as key to potency [Re: Interested]
    #4686371 -

I really don't have time for a proper post, so just quick hits:
Ammonium succinate is expensive. It can be easily and cheaply synthesized from succinic acid and ammonium. I would consult the proper "Current Protocols in ... " series for hints of why that particular N source was chosen. In the very least it should provide pointers for further research. As for tryptophan decarboxylase - make sure the data applies to Psilocybe's enzyme, which I would suspect might be evolutionary convergent (analogous) to those of plants, and thus could be subject to different regulation (also, some inhibitory regulations can be overwhelmed by excess substrate). Since the biochemical pathway to psilo. isn't well delineated, one would have to rely on empirical data anyway. But I wouldn't extrapolate the paucity of empiricla data we do have - in this case, beyond 11 days and liquid culture. Limiting ourselves to that offers interesting enough venues.

Extras: Filter Print Post Top
Re: Nitrogen as key to potency [Re: stvip]
    #4711711 -

At least slightly related.

Use of different nitrogen sources by the edible ectomycorrhizal mushroom Cantharellus cibarius
J. Ignacio Rangel-Castro ? Eric Danell Andy F. S. Taylor

Abstract The growth of three strains of Cantharellus cibarius on liquid media containing ammonium, nitrate and bovine serum albumin (BSA) in different combinations was determined. The most readily utilisable source of N was ammonium. BSA utilisation was limited compared with media containing ammonium. Growth on nitrate was also poor, suggesting a limited capacity of C. cibarius to metabolise this nitrogen source. There was some indication of considerable intraspecific variation within C. cibarius in the utilisation of nitrogen sources.

Possible links between atmospheric nitrogen deposition and the observed decrease of sporocarp formation by C. cibarius in Europe are discussed. We highlight the potential ecological significance of bacteria associated with C. cibarius which may circumvent the need for fungal extracellular enzymes to access complex nitrogen sources.
Keywords Cantharellus cibarius ? Organic nitrogen utilisation ? Minerotrophic fungi ? Sporocarp production

http://www-mykopat.slu.se/Newwebsite/mycorrhiza/kantarellfiler/texter/nitrogen.pdf

Extras: Filter Print Post Top
Re: Nitrogen as key to potency [Re: scatmanrav]
    #4711718 -

Ponder that P. Cubensis is historically referred to as a "DUNG LOVER".

There is a lot of data (Penn State - for instance) that a compatible Nitrogen content, all else being optimal - under ideal conditions results in optimal crops.

Stands to reason, optimal fruits - give optimal (natural) potency.

High "N" H/poo or h/poo / dung based composts have given me - what I would consider optimal (natural) potency.

Nothing but a lot of grows - over a lot of years - to back that up.

But, that is enough for me.


--------------------

Extras: Filter Print Post Top
Re: Nitrogen as key to potency [Re: Interested]
    #4735076 -

Interested - I understand where you coming from I think - but Agar's post makes me think that what I said originally makes sense as well - Im confused :frown:


--------------------

Extras: Filter Print Post Top
Jump to top Pages: | 1 | 2 | 3  [ show all ]

Shop: Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* bacteria, nitrogen & fermentation... SN122A 2,115 7 03/20/11 09:42 PM
by 3n1gm4
* Scott's 3 in 1, use Bonemeal to boost nitrogen
( 1 2 all )		 Visigoth 4,431 22 05/29/03 11:21 AM
by Anno
* Nitrogen in substrate anarchOi 1,306 3 04/17/08 07:13 PM
by Mr E Guest
* nitrogen requirement in liquid culture wood chip 1,793 17 12/12/09 01:57 AM
by wood chip
* Nitrogen as a limiting nutrient thisspock 1,907 4 02/18/04 11:00 PM
by Joshua
* Adding nitrogen to substrate Gumby79 1,675 3 07/18/03 09:33 AM
by mycofile
* Liquid Nitrogen dehydration STINKSTER 2,139 8 10/29/08 04:16 PM
by denger
* Testing For Psilocybin Potency Using TLC
( 1 2 3 4 5 all )		 AdoreChampignons 20,497 82 06/02/09 12:10 AM
by fastfred

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta
12,445 topic views. 0 members, 5 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.005 seconds on 15 queries.