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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Wild Bird Seed

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Offlinekingfish4200
shroom diddy

Registered: 08/12/05
Posts: 673
Last seen: 5 years, 2 months
liquid culture question
    #4731314 - 09/29/05 01:35 PM (11 years, 2 months ago)

Yesterday I knocked up 8 quart jars of bird seed and 1 liquid culture (baby bottle Method) with 1 10cc syringe of koh sumi. I knocked up the each quart jar with 1cc spore solution (I know that isn't much but but I figure it's a fast colonizer so that may make up for it and I'm in no rush.) For my liquid culture, I used 2cc spore solution. I put it in the PC for 20 min's @ 15psi. When I got it out the water looked like it had a yellowish color to it. Is that normal? Also do you think the jars will be fine? About how long do you think till I might see first signs of growth?(thay are in a TIT at about 82F) This is my first bulk grow and I have never used bird seed. I have only used PF style cakes in the past.


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"The answer is never the answer. What's really interesting is the mystery. If you seek the mystery instead of the answer, you'll always be seeking. I've never seen anybody really find the answer-- they think they have, so they stop thinking. But the job is to seek mystery, evoke mystery, plant a garden in which strange plants grow and mysteries bloom. The need for mystery is greater than the need for an answer."

-- Ken Kesey


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Offlineready2go
Eternal

Registered: 07/19/05
Posts: 249
Loc: Up the street and around ...
Last seen: 5 years, 4 months
Re: liquid culture question [Re: kingfish4200]
    #4731328 - 09/29/05 01:40 PM (11 years, 2 months ago)

You did PC befor inoculation right?


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Offlinekingfish4200
shroom diddy

Registered: 08/12/05
Posts: 673
Last seen: 5 years, 2 months
Re: liquid culture question [Re: ready2go]
    #4731386 - 09/29/05 01:49 PM (11 years, 2 months ago)

yes. I PC'ed for 20min's @ 15psi. Let it cool overnite then knoked it up in a glove box. The water was yellowish collor right after comeing out of the pc.


--------------------
"The answer is never the answer. What's really interesting is the mystery. If you seek the mystery instead of the answer, you'll always be seeking. I've never seen anybody really find the answer-- they think they have, so they stop thinking. But the job is to seek mystery, evoke mystery, plant a garden in which strange plants grow and mysteries bloom. The need for mystery is greater than the need for an answer."

-- Ken Kesey


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Offlineready2go
Eternal

Registered: 07/19/05
Posts: 249
Loc: Up the street and around ...
Last seen: 5 years, 4 months
Re: liquid culture question [Re: kingfish4200]
    #4731400 - 09/29/05 01:51 PM (11 years, 2 months ago)

Honey,karo, what..... anyhow the sugars can carmelize in the PC as long as its only a pale yellow you should be ok..... no heavey floaters or anything right?


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 4 hours, 18 minutes
Trusted Cultivator
Re: liquid culture question [Re: kingfish4200]
    #4731439 - 09/29/05 01:57 PM (11 years, 2 months ago)

What is the LC made from ?

What are the components ?

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


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Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

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InvisibleFungusMan
I81U812
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Re: liquid culture question [Re: HippieChick]
    #4731459 - 09/29/05 02:00 PM (11 years, 2 months ago)

Hey my fish buddy, lol. Yellowing is caused by caramelization of the sugars. You should be just fine.


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Offlinekingfish4200
shroom diddy

Registered: 08/12/05
Posts: 673
Last seen: 5 years, 2 months
Re: liquid culture question [Re: kingfish4200]
    #4731463 - 09/29/05 02:01 PM (11 years, 2 months ago)

200cc's water 6cc's karo
here ia a pic.



also in info for some of my outher questions?


--------------------
"The answer is never the answer. What's really interesting is the mystery. If you seek the mystery instead of the answer, you'll always be seeking. I've never seen anybody really find the answer-- they think they have, so they stop thinking. But the job is to seek mystery, evoke mystery, plant a garden in which strange plants grow and mysteries bloom. The need for mystery is greater than the need for an answer."

-- Ken Kesey


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Offlinekingfish4200
shroom diddy

Registered: 08/12/05
Posts: 673
Last seen: 5 years, 2 months
Re: liquid culture question [Re: kingfish4200]
    #4731471 - 09/29/05 02:03 PM (11 years, 2 months ago)

whats up fungisman been a little while...lol I've been busy :wink: ....lol
as you can see


--------------------
"The answer is never the answer. What's really interesting is the mystery. If you seek the mystery instead of the answer, you'll always be seeking. I've never seen anybody really find the answer-- they think they have, so they stop thinking. But the job is to seek mystery, evoke mystery, plant a garden in which strange plants grow and mysteries bloom. The need for mystery is greater than the need for an answer."

-- Ken Kesey


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InvisibleFungusMan
I81U812
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Folding@home Statistics
Registered: 08/06/05
Posts: 3,109
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Re: liquid culture question [Re: kingfish4200]
    #4731483 - 09/29/05 02:05 PM (11 years, 2 months ago)

Cmon fish. Your not a newb..."How long will it take?".5-12 days to see noticable myc growth.


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Offlinekingfish4200
shroom diddy

Registered: 08/12/05
Posts: 673
Last seen: 5 years, 2 months
Re: liquid culture question [Re: FungusMan]
    #4731514 - 09/29/05 02:12 PM (11 years, 2 months ago)

thats what i was thinking..but with only useing 1cc in a quart jar i didnt know for sure like i said ive only done pf style cakes in the past in half pint jars useing about 2cc's in 1 half pint. that took about 5 to 7 days like that. but 1cc in a quart jar of bird seed i wasnt sure.


--------------------
"The answer is never the answer. What's really interesting is the mystery. If you seek the mystery instead of the answer, you'll always be seeking. I've never seen anybody really find the answer-- they think they have, so they stop thinking. But the job is to seek mystery, evoke mystery, plant a garden in which strange plants grow and mysteries bloom. The need for mystery is greater than the need for an answer."

-- Ken Kesey


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Wild Bird Seed

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