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OfflineAKSE
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Question regarding spores...
    #4727194 - 09/28/05 09:48 PM (12 years, 1 month ago)

Hey everyone. Would it be possible to cultivate mushrooms from spores left in the bottom of the bag (when bought)? I know there are are most likely hardly any spores in the dust at the bottom, but just wondering if cultivation is possible...thanks!


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OfflineBamaman
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Re: Question regarding spores... [Re: AKSE]
    #4727213 - 09/28/05 09:53 PM (12 years, 1 month ago)

From what I understand the chances of success are slim, assuming those are actually viable spores in the bag.... they have not been treated in the proper manner and will probably contain contams.


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Offlinegoofy98
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Re: Question regarding spores... [Re: Bamaman]
    #4727372 - 09/28/05 10:28 PM (12 years, 1 month ago)

will have to isolate on perozide agar first then transfer to anti bacterial agar and then isolated until no contams. THEN you can you it. good luck because it is a very advance technique. but its not impossible. there are a tek or two on agar you should read. but save yourself time and money and order from ralphsters.


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InvisibleFooManM
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Re: Question regarding spores... [Re: goofy98]
    #4727442 - 09/28/05 10:37 PM (12 years, 1 month ago)

Peroxide will kill spores. To use peroxided agar, you need live mycelium. You can try to drop the spores on an agar plate (no peroxide) in as sterile conditions as possible and try to grow out myc on the plate. Doing it this way, you could isolate myc from contams. It would be easier to just buy spores from a vendor though.


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InvisibleFooManM
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Re: Question regarding spores... [Re: AKSE]
    #4727448 - 09/28/05 10:38 PM (12 years, 1 month ago)

What type of bag are you talking about? A bag that had a print in it?


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Invisibledoubledutch
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Re: Question regarding spores... [Re: FooMan]
    #4729319 - 09/29/05 03:09 AM (12 years, 1 month ago)

I think he's talking about when you buy cubes off the street, if there's spores in the bottom of the bag.


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InvisibleFooManM
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Re: Question regarding spores... [Re: doubledutch]
    #4730051 - 09/29/05 07:54 AM (12 years, 1 month ago)

Ah Ha! Makes sense to me, it just didn't occur to me that anyone on this site would still buy shrooms anymore :smirk:


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InvisibleRoadkillM
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Re: Question regarding spores... [Re: AKSE]
    #4730375 - 09/29/05 10:35 AM (12 years, 1 month ago)

Yes its possible...but it might be a pain in the ass...
and you might just get contams.

You would make up some anti bacterial agar and try to get some healthy mycelium growing without any contams.

Then after you get some healthy mycelium growing...do some transfers to either some peroxided agar or some regular agar recipy like MEA or PDYA.

After you have a really good isolate growing on agar...you would cut out some agar wedges and add them to your substrate jars and let them colonize the jars.

It would be much easier for you to just buy some viable spore syringes from a Vendor.

tc


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PM the names with on there names, that means they have mushrooms for sale.



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