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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Pressure Cooker, Vermiculite

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Offline_JJ_
PsychedelicRevolutionary
Registered: 06/12/01
Posts: 369
Loc: NSW, Australia
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Why???? Nooo!! :((
    #472215 - 11/28/01 11:36 AM (15 years, 13 days ago)

OK I have now attempted this a total of 5 times. EVERY single time bar one in which I used agar to innoculate has resulted in green mould (the agar innoculation gave me some weird brownish red contam). And EVERY single time a similar thing happends. I have been extremely careful in sterilizing everything for the last 3 times, given my first 2 failures.
It goes something like this.. the spores germinate properly and start to spread around the cake. Then, within the first week yellow spots appear and these start to grow green mould. I pressure cooked the last two batches for over 1 and a half hours as a precaution. The innoculation was done as carefully as possible, with minimal disturbance to the vermiculite layer.
That leaves only two possible options: either the spore print is dirty OR the syringe is not prepared properly for innoculation.
I use the oven as a 'hood' to prepare the syringe and make sure the area is as clean as possible. It's a brand new house btw, no carpet. So a dirty area can't really be the problem can it? I flame the needle between between every single step just to make sure its sterile. The syringes were brand new.
If anyone knows any fail-proof syringe techniques please detail them!! This is really getting me down :((
That leaves only one other thing - the spore print. I obtained it from Sporeworks who, I understand, are very reliable. I guess I could have dirtied the spore print myself.. although I have been extremely careful.
Something else interesting.. when the green starts to show up it looks as if it's uniformingly starting everywhere across the jar. Is this just its nature - that it spreads around first before turning green? It's like there's a hundred different germination points and all the bits turn yellow then green.
*sigh*
Thanks to anyone who helps!! :)


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OfflineAnnoA
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Re: Why???? Nooo!! :(( [Re: _JJ_]
    #472453 - 11/28/01 04:40 PM (15 years, 12 days ago)

>when the green starts to show up it looks as if it's
>uniformingly starting everywhere across the jar.

This tells me that the spores are not the problem. If the spores are contaminated, the contamination first shows around the inoculation points.

Now, what can be the reasons:

- Are you sure the pressure cooker is working properly? Is the outside of the pressure cooker hotter then the ambient boiling temperature of water? If you put a drop of water on the outside of the PC while it?s under pressure, the drop should evaporate while boiling . Does it do that?

- Do you take the jars out of the pressure cooker and leave in the open to cool down? Don?t do that, leave them in the presure cooker to cool down.

- are there any major temperature fluctuations where you store the jars? There shouldn?t be.

- Perhaps the rice you use has a high spore count. Try using a different brand.


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OfflineHammerloaf
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Registered: 11/26/01
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Re: Why???? Nooo!! :(( [Re: Anno]
    #472469 - 11/28/01 04:57 PM (15 years, 12 days ago)

I am a newbie at this too.. in fact, I've got jars just about ready for birthing, with 0% contam's... 8 of them.. *brag,brag*.. well, it is my first time :)

a couple of suggestions for u..

1. pre-sterilize the raw ingredients if your using a brf/vermiculite cake... consult the MMGG for the details

2. Use a glove box refer to:

http://www.shroomery.org/scgt/SCGTv0.3.html

these are both precautions I took, and thus far everything is perfect.


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Offline_JJ_
PsychedelicRevolutionary
Registered: 06/12/01
Posts: 369
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Re: Why???? Nooo!! :(( [Re: Anno]
    #472898 - 11/28/01 10:44 PM (15 years, 12 days ago)

>- Are you sure the pressure cooker is working properly? Is the outside of the pressure cooker hotter then the ambient boiling temperature of water? If you put a drop of water on the outside of the PC while it?s under pressure, the drop should evaporate while boiling . Does it do that?
It does. I know a LOT of pressure is built up inside the cooker (I tried tapping the release valve, high-pitched whine, lots of steam).
>- Do you take the jars out of the pressure cooker and leave in the open to cool down?
No I didn't.

>- are there any major temperature fluctuations where you store the jars? There shouldn?t be.
Day/night fluctuations. That's all.

>- Perhaps the rice you use has a high spore count. Try using a different brand.
I've tried both ready-made brown rice flour and brown rice. :(

Any other suggestions? :)

Hammerloaf:
>1. pre-sterilize the raw ingredients if your using a brf/vermiculite cake
Do you mean pressure cook the ingredients separately and then mix up the stuff and cook the jars?

>2. Use a glove box
Well I used the oven tek - I'll try a proper (cardboard heh) glovebox next time then.

Thanks!!




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OfflineAnnoA
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Re: Why???? Nooo!! :(( [Re: _JJ_]
    #473192 - 11/29/01 03:19 AM (15 years, 12 days ago)

>Do you mean pressure cook the ingredients separately and
>then mix up the stuff and cook the jars?

No, to bake them(dry) before mixing.


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Offlineboomboomboomboom
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Registered: 11/29/01
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Re: Why???? Nooo!! :(( [Re: _JJ_]
    #473209 - 11/29/01 03:39 AM (15 years, 12 days ago)

cook up a few jars, don't knock them up. Let them set a couple weeks, see if they contam. If not, it's not your cooking/cooling procedure probably. That would mean you're introducing contams during innoculation.


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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Pressure Cooker, Vermiculite

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