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Amazon Shop: Agar

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Invisiblesyntonik
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Registered: 08/30/05
Posts: 46
Agar Question...
    #4715436 - 09/26/05 06:34 PM (12 years, 1 month ago)

Ok, if you're trying to isolate a single genome strain, at what point should you take it out of incubation? When the mycelium is completely covered over the agar or when the growth is significant enough to cut out a nice peice? Any help on this would be greatly appreciated...Thanks!


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Invisiblesyntonik
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Registered: 08/30/05
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Re: Agar Question... [Re: syntonik]
    #4719032 - 09/27/05 11:05 AM (12 years, 1 month ago)

Anyone have an answer???


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InvisibleFooManM
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Re: Agar Question... [Re: syntonik]
    #4719247 - 09/27/05 12:17 PM (12 years, 1 month ago)

If you are using the mycelium as an inoculant, then just cut a small piece out for that purpose, then incubate the plate until the remainder is full. You can store the plate in the fridge for future use.


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Quick WBS Prep


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Invisiblebackupwards
peon

Registered: 04/02/05
Posts: 3,022
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Re: Agar Question... [Re: FooMan]
    #4719267 - 09/27/05 12:23 PM (12 years, 1 month ago)

it would take a bit of work to isolate out a single genome as you suggest. you would pull pieces from the original plate and let them grow out on another plate and then continue that process until you are getting the myc growth that you desire. something like that anyway.
:peace:


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Invisiblesyntonik
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Registered: 08/30/05
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Re: Agar Question... [Re: backupwards]
    #4720556 - 09/27/05 05:09 PM (12 years, 1 month ago)

Ah ok, so the first dish wouldn't have to be completely covered, just enough so that I can pull some myc off and put it in the next dish...right?


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OfflineKosher
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Re: Agar Question... [Re: syntonik]
    #4720745 - 09/27/05 05:38 PM (12 years, 1 month ago)

Yeah eric, you got it.
My aim when i use agar is to keep isolating till i'm happy with what's on the newest plate. Then i inoculate a liquid culture with my new isolated strain.


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Amazon Shop: Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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