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Offlineswiftrance
Let there belight

Registered: 03/21/05
Posts: 449
Last seen: 5 years, 1 month
G2G transfers. How many?
    #4699405 - 09/23/05 08:36 AM (12 years, 4 days ago)

Hey guys whats happenin.

Say you inoculated a qt jar with spores from a syringe. then took that colonized jar and used it for 5more jars, then one of those 5, for 5more. kinda see what i mean? How many times are you allowed to do this before you start getting, i dunno, like wierd mutations n stuff?


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Offlinegoofy98
Heart of ahustler wrist ofa chemist
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Registered: 03/18/05
Posts: 451
Loc: next door
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Re: G2G transfers. How many? [Re: swiftrance]
    #4699411 - 09/23/05 08:46 AM (12 years, 4 days ago)

3 generations of grain to grain is typically what the pros do. The genetics seem to start to degrade noticably after this.


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InvisibleOmnicracker
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Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: G2G transfers. How many? [Re: goofy98]
    #4699716 - 09/23/05 11:03 AM (12 years, 4 days ago)

is the 3 generations rule just for g2g, what if you take a fresh mushroom and used mush water to inoculate more jars? that should be fine, right? i dont want to have to buy more spores...


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Invisibleagar
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Re: G2G transfers. How many? [Re: Omnicracker]
    #4699768 - 09/23/05 11:17 AM (12 years, 4 days ago)

Search "LC" (liquid culture).
You can use a single ml of spore solution to make 500 LC syringes.


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Offlinethenewguy05
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Registered: 02/11/05
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Re: G2G transfers. How many? [Re: agar]
    #4699950 - 09/23/05 12:10 PM (12 years, 4 days ago)

you can always take a print when you get mushrooms, then you won't have to buy more spores.


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Offlinebleedforthis
ill bleed foryou
Registered: 07/28/05
Posts: 92
Loc: Looking down from above.
Last seen: 11 years, 8 months
Re: G2G transfers. How many? [Re: thenewguy05]
    #4700576 - 09/23/05 03:07 PM (12 years, 4 days ago)

where can i learn to make liquid culture? i've always wondered what LC meant .


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InvisibleFooManM
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Registered: 02/02/05
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Re: G2G transfers. How many? [Re: bleedforthis]
    #4700955 - 09/23/05 04:26 PM (12 years, 4 days ago)

Quote:

bleedforthis said:
where can i learn to make liquid culture? i've always wondered what LC meant .




Quote:

agar said:
Search "LC" (liquid culture)




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OfflineMycophilic
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Registered: 09/17/05
Posts: 104
Loc: WA, USA
Last seen: 7 years, 8 months
Re: G2G transfers. How many? [Re: Omnicracker]
    #4701044 - 09/23/05 04:44 PM (12 years, 4 days ago)

i might recommend cloning a good sterile fresh young shroom, you can clone that into as many jars as you can extract fresh tissue from the shroom, and then each of those jars can be replicated, and probably replicated again


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OfflineInjectTruth
Wasting my Time,Waiting for theEnd
Male

Registered: 10/05/03
Posts: 778
Loc: New Jerusalem
Last seen: 8 years, 9 months
Re: G2G transfers. How many? [Re: Mycophilic]
    #4753238 - 10/04/05 10:21 AM (11 years, 11 months ago)

I've always wondered, with LC, is ALL the liquid good to use?

No matter how hard I shake, there is always some liquid leftover with no visible mycelium in it. Can this liquid still be used to inoculate?


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