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Offlinecrispyburrito
member

Registered: 08/29/01
Posts: 135
Last seen: 21 years, 10 months
Steralizing
    #469915 - 11/26/01 10:34 AM (22 years, 4 months ago)

What would be the best way to steralize a used spore srynge ? Should you fill it with water then p.c or leave it withought water ? How long should it be in the p.c for ??? Thanks


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This statment or question is fictional and for fun not to be used in real life. Just a hypothetical situation..

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OfflineAnnoA
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Registered: 06/17/99
Posts: 24,166
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Last seen: 4 days, 19 hours
Re: Steralizing [Re: crispyburrito]
    #469920 - 11/26/01 10:41 AM (22 years, 4 months ago)

In a pressure cooker for 20 minutes. The syringe can be empty, but don?t forget to put water in the pressure cooker.

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Offlinecrispyburrito
member

Registered: 08/29/01
Posts: 135
Last seen: 21 years, 10 months
Re: Steralizing [Re: Anno]
    #469922 - 11/26/01 10:47 AM (22 years, 4 months ago)

Great thanks for the help Anno !


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This statment or question is fictional and for fun not to be used in real life. Just a hypothetical situation..

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Offlineupupup
guardian

Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 1 month
Re: Steralizing [Re: crispyburrito]
    #469938 - 11/26/01 11:12 AM (22 years, 4 months ago)

I suck alcohol into my syrnges and shake them around a bit then expell the alcohol. Then I suck 3 full syringes of boiling water into them and expell that into the sink. Then I refill them one more time and keeping that sterile water in the syringe, I wrap the syringe in foil and steam/boil for an hour. Then I have a sterile syringe with sterile water in it to add to the spores when I make my innoculant. Another thing I do for extra security is to pour a bit of alcohol into the syrnge needle cap and stick the needle into that when I store them. Then when I flame sterylize I just pour that out and ignite that to sterylize my needle before each innoculation.


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Support bacteria - they're the only culture some people have.

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