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Offlinecrispyburrito
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Registered: 08/29/01
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Steralizing
    #469915 - 11/26/01 12:34 PM (15 years, 12 days ago)

What would be the best way to steralize a used spore srynge ? Should you fill it with water then p.c or leave it withought water ? How long should it be in the p.c for ??? Thanks


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OfflineAnnoA
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Re: Steralizing [Re: crispyburrito]
    #469920 - 11/26/01 12:41 PM (15 years, 12 days ago)

In a pressure cooker for 20 minutes. The syringe can be empty, but don?t forget to put water in the pressure cooker.


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Offlinecrispyburrito
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Registered: 08/29/01
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Last seen: 14 years, 6 months
Re: Steralizing [Re: Anno]
    #469922 - 11/26/01 12:47 PM (15 years, 12 days ago)

Great thanks for the help Anno !


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Offlineupupup
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Re: Steralizing [Re: crispyburrito]
    #469938 - 11/26/01 01:12 PM (15 years, 12 days ago)

I suck alcohol into my syrnges and shake them around a bit then expell the alcohol. Then I suck 3 full syringes of boiling water into them and expell that into the sink. Then I refill them one more time and keeping that sterile water in the syringe, I wrap the syringe in foil and steam/boil for an hour. Then I have a sterile syringe with sterile water in it to add to the spores when I make my innoculant. Another thing I do for extra security is to pour a bit of alcohol into the syrnge needle cap and stick the needle into that when I store them. Then when I flame sterylize I just pour that out and ignite that to sterylize my needle before each innoculation.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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