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Registered: 10/31/01
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2nd Generation...
    #469812 - 11/26/01 08:39 AM (15 years, 3 months ago)

I am awating prints for Cambos and will be making my own syringes and cultivating. Once these are mature I will make my own prints. Is is correct to assume that these 2nd generation cambos will be somewhat "inferior" to the 1st generation? Should I clone the 1st generation instead?


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Registered: 01/07/01
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Re: 2nd Generation... [Re: Mklangelo]
    #469820 - 11/26/01 09:03 AM (15 years, 3 months ago)

i think you'd have better luck with honey-water or taking prints. Cloning is iffy, unless you're handy with agar and have a good glovebox.

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Registered: 11/14/01
Posts: 1,253
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Re: 2nd Generation... [Re: Mklangelo]
    #469870 - 11/26/01 11:38 AM (15 years, 3 months ago)

Here's a simple method to cloning. This is what dude does.

1. prepare your honey water jar normally.
2. prepare sterile syringe with water inside (this would probably work w/o any water in the syringe, but dude has always done it with).
3. inside your glovebox, peel the stem of your desired mushroom to clone, back to the cap.
4. take a core sample of the mushroom stem(the inside\ never touched part) with your syringe.(DON'T suck core sample into the syringe, just leave it in the needle tip)
5. inject core sample into your honeywater jar.

within a few days, you will see mycelium starting to grow from the fragment you cored and injected. Treat as regular honeywater. You now have isolated mycelium, cloned from your chosen fruit, without the hassles of agar.

BTW: dude uses nothing more than a cleaned and lysol-sprayed 10gal. aquarium, covered with plastic wrap for his glove box. He has accomplished about a 99% success rate for making syringes and a 90-95% success rate for cloning using this method.


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The Dr.
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Re: 2nd Generation... [Re: Mklangelo]
    #469880 - 11/26/01 11:48 AM (15 years, 3 months ago)

I could be mistaken as i have never cloned myself. I'm fairly sure that the clone would be the second generation and somewhat inferior. The print would be just as the first. Think about how many generations the mushroms has gone though throughout history though spore procreation. you think they get inferior though time? If i'm wrong someone reply to this thred and let me know. if i am i would LOVE to see the original F1 of the specie.

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Registered: 11/04/01
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Re: 2nd Generation... [Re: Scourge]
    #469888 - 11/26/01 11:57 AM (15 years, 3 months ago)

I know that according to what many people have said in the past is that the more generations taken can affect the new mushrooms. This usually happens when the shroomies are grown on the same substrate over and over and over and over again. When this happens, according to what I have read, the mycelium will take liking to this certain kind of substrate and not do so well on others and possibly lose strength, but I haven't experimented, and it would probably take many years for any recognizable difference in the mushrooms.

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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