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Offlinemushisgood_420
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How would one go about making and lc w/o a pc?
    #4697999 - 09/23/05 12:40 AM (11 years, 2 months ago)

My pressure cooker will not be arriving for a week and I was wondering if there is anyway I could sterilize a liquid culture and innoc with the prints that I already have?? (or is this even a good idea) I figure that if I have 8 jars of liquid culture going for a week I could just pressure cook jars and oven bags of my cracked corn substrate and then innoc. them with liquid culture insteada my spores. Help would be appretiated.


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Invisiblemskip23
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Re: How would one go about making and lc w/o a pc? [Re: mushisgood_420]
    #4698028 - 09/23/05 12:44 AM (11 years, 2 months ago)

Get plastic lids and nuke it or do a search for the baby bottle tek


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OfflinemushboyS
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Re: How would one go about making and lc w/o a pc? [Re: mskip23]
    #4698618 - 09/23/05 01:56 AM (11 years, 2 months ago)

i just made some buy loading into a pint jar and mirco'ed it for about 2minutes. let cool and put a few kernels of colonized wbs in the mix. so far i have a nice goo that looks like semem...


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Offlineblackout
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Re: How would one go about making and lc w/o a pc? [Re: mushboy]
    #4699134 - 09/23/05 04:21 AM (11 years, 2 months ago)

search
+microwave +lc
2mins is not enough


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InvisibleFooManM
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Re: How would one go about making and lc w/o a pc? [Re: mushboy]
    #4699319 - 09/23/05 06:50 AM (11 years, 2 months ago)

Quote:

mushboy said:
i just made some buy loading into a pint jar and mirco'ed it for about 2minutes.




In addition to the time being too short, this won't work with jars with metal lids. I know it seems like common sense, but some dumbass will try it if you don't say anything. :smirk:


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Offlinebob_smith93456
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Registered: 07/28/05
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Re: How would one go about making and lc w/o a pc? [Re: FooMan]
    #4699345 - 09/23/05 07:25 AM (11 years, 2 months ago)

I used this tek, but I used a pint jar instead of the plastic thing. I also used 2 layers of saran wrap, drilled 2 1/4" holes in the lid and stuffed them with polyfill. This way I protect the inoculation point and the point where I fill my syringes. Good Luck.

http://www.shroomery.org/forums/showflat...rt=all&vc=1


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Offlinebob_smith93456
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Re: How would one go about making and lc w/o a pc? [Re: bob_smith93456]
    #4699346 - 09/23/05 07:27 AM (11 years, 2 months ago)

As foomanshroom suggested, make sure to keep the lid off when the jar's in the microwave :smile:.  Once it's done microwaving then put the lid on the jar :smile:


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Offlineblackout
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Re: How would one go about making and lc w/o a pc? [Re: FooMan]
    #4699361 - 09/23/05 07:43 AM (11 years, 2 months ago)

Quote:

FooManShroom said:
Quote:

mushboy said:
i just made some buy loading into a pint jar and mirco'ed it for about 2minutes.




In addition to the time being too short, this won't work with jars with metal lids. I know it seems like common sense, but some dumbass will try it if you don't say anything. :smirk:




I must be a dumbass so.... an informed dumbass about metal in microwaves.


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Offlinerygo796
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Re: How would one go about making and lc w/o a pc? [Re: blackout]
    #4699571 - 09/23/05 10:15 AM (11 years, 2 months ago)

do a search for "microwave karo tek"

4mL Karo syrup
96mL water
cover gladware with plastic wrap microwave 5min.
Inject spores and cover

if i remember correctly.


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Offlinej_db69
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Re: How would one go about making and lc w/o a pc? [Re: rygo796]
    #4699653 - 09/23/05 10:44 AM (11 years, 2 months ago)

just don't seal the lid, you have to have it cracked a little if you using the gladware covers.


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