Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Autoclave, Petri Dish

Jump to first unread post. Pages: 1
Invisiblesyntonik
Stranger

Registered: 08/30/05
Posts: 46
Do you absolutely have to sterilize Agar?
    #4693625 - 09/22/05 02:44 AM (12 years, 6 months ago)

I was just wondering, is it absolutely necessary that you PC your Agar? Even the kind you buy in a bottle from a science store?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePierre
Stranger
Registered: 09/18/05
Posts: 124
Last seen: 12 years, 6 months
Re: Do you absolutely have to sterilize Agar? [Re: syntonik]
    #4693805 - 09/22/05 04:00 AM (12 years, 6 months ago)

try not sterilizing it and see what happens :wink:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineblackout
Microwaver
 User Gallery

Registered: 07/17/00
Posts: 4,901
Last seen: 19 days, 7 hours
Trusted Cultivator
Re: Do you absolutely have to sterilize Agar? [Re: Pierre]
    #4693828 - 09/22/05 04:16 AM (12 years, 6 months ago)

If it is presterilized in the bottle you just have to warm it up and pour it in a sterile petri dish, preferably in a glove box.


--------------------
support the free spore ring http://fsre.nl/


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinebrain_dam
Stranger
Registered: 09/24/05
Posts: 4
Last seen: 12 years, 6 months
Re: Do you absolutely have to sterilize Agar? [Re: blackout]
    #4705353 - 09/24/05 03:33 PM (12 years, 6 months ago)

-i like better to sterylize agar when it is already in petri dish or testing tube - that gives 100% non contaminated medium... I really dont see the difference in sterilizing medium in bootle or in petri dishes-i just put them in plastic-bag, and boil twice ... in water

I had once petri dishes made from some kinde of 'plastic' prabobly PP ... they was for sterylization (that was write on them) in autoclave in. temp.140C ... i was boiling them, and they dis formed - changed shape :frown: ... glas dishes ar best ...


Edited by brain_dam (09/24/05 03:36 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJabberw0cky
Misanthropist

Registered: 08/01/05
Posts: 158
Loc: Colorado - The asshole of...
Last seen: 11 years, 6 months
Re: Do you absolutely have to sterilize Agar? [Re: brain_dam]
    #4705553 - 09/24/05 04:10 PM (12 years, 6 months ago)

Yes... Generally plastic does melt. -nods-
-Jabber


--------------------
"Beware the Jabberwock, my son!
The jaws that bite, the claws that catch!
Beware the Jubjub bird, and shun
The frumious Bandersnatch!"
He took his vorpal sword in hand:
Long time the manxome foe he sought --
So rested he by the Tumtum tree,
And stood awhile in thought.
Once upon a looking glass...


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Do you absolutely have to sterilize Agar? [Re: brain_dam]
    #4705594 - 09/24/05 04:18 PM (12 years, 6 months ago)

Quote:

brain_dam said:
-i like better to sterylize agar when it is already in petri dish or testing tube




boil overs are a big problem there.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Autoclave, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Post deleted by Moe Howard vampirism 426 3 11/23/02 04:15 PM
by resin
* Re: Agar & Petri Dish Problem MrBoomer 1,148 3 04/16/01 11:49 PM
by Anno
* sterilizing agar W/O a pc staindblue 902 2 07/06/02 09:36 AM
by staindblue
* (?) How to sterilize agar-agar World Spirit 1,431 16 02/10/02 03:58 PM
by SThomas
* agar
( 1 2 all )
mikearch 1,343 21 10/05/02 03:52 PM
by mikearch
* How is the best way to pressure cook Petri Dishes? SafeHaven 2,546 12 05/10/03 03:45 PM
by MycoFactory
* Re: Petri Dishes & Agar Q's Placebo 1,995 5 12/07/99 12:00 PM
by Anonymous
* Re: sterilization q's Anonymous 345 2 02/16/00 08:27 PM
by ar393

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
3,329 topic views. 14 members, 73 guests and 31 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:

Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.007 seconds on 19 queries.