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Amazon Shop for: Agar, Autoclave, Petri Dish

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Invisiblesyntonik
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Registered: 08/30/05
Posts: 46
Do you absolutely have to sterilize Agar?
    #4693625 - 09/22/05 02:44 AM (11 years, 2 months ago)

I was just wondering, is it absolutely necessary that you PC your Agar? Even the kind you buy in a bottle from a science store?


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OfflinePierre
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Registered: 09/18/05
Posts: 124
Last seen: 11 years, 2 months
Re: Do you absolutely have to sterilize Agar? [Re: syntonik]
    #4693805 - 09/22/05 04:00 AM (11 years, 2 months ago)

try not sterilizing it and see what happens :wink:


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Offlineblackout
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Re: Do you absolutely have to sterilize Agar? [Re: Pierre]
    #4693828 - 09/22/05 04:16 AM (11 years, 2 months ago)

If it is presterilized in the bottle you just have to warm it up and pour it in a sterile petri dish, preferably in a glove box.


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Offlinebrain_dam
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Registered: 09/24/05
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Re: Do you absolutely have to sterilize Agar? [Re: blackout]
    #4705353 - 09/24/05 03:33 PM (11 years, 2 months ago)

-i like better to sterylize agar when it is already in petri dish or testing tube - that gives 100% non contaminated medium... I really dont see the difference in sterilizing medium in bootle or in petri dishes-i just put them in plastic-bag, and boil twice ... in water

I had once petri dishes made from some kinde of 'plastic' prabobly PP ... they was for sterylization (that was write on them) in autoclave in. temp.140C ... i was boiling them, and they dis formed - changed shape :frown: ... glas dishes ar best ...


Edited by brain_dam (09/24/05 03:36 PM)


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OfflineJabberw0cky
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Registered: 08/01/05
Posts: 158
Loc: Colorado - The asshole of...
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Re: Do you absolutely have to sterilize Agar? [Re: brain_dam]
    #4705553 - 09/24/05 04:10 PM (11 years, 2 months ago)

Yes... Generally plastic does melt. -nods-
-Jabber


--------------------
"Beware the Jabberwock, my son!
The jaws that bite, the claws that catch!
Beware the Jubjub bird, and shun
The frumious Bandersnatch!"
He took his vorpal sword in hand:
Long time the manxome foe he sought --
So rested he by the Tumtum tree,
And stood awhile in thought.
Once upon a looking glass...


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InvisiblePrisoner#1M
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Re: Do you absolutely have to sterilize Agar? [Re: brain_dam]
    #4705594 - 09/24/05 04:18 PM (11 years, 2 months ago)

Quote:

brain_dam said:
-i like better to sterylize agar when it is already in petri dish or testing tube




boil overs are a big problem there.


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Amazon Shop for: Agar, Autoclave, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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