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Amazon Shop for: ½ Pint Jars, Rye Grain, Vermiculite, Wild Bird Seed

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OfflineMycoGod
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Registered: 09/14/05
Posts: 9
Last seen: 10 years, 5 months
0 Growth 0 Contaminates
    #4690373 - 09/21/05 03:30 PM (11 years, 5 months ago)

Alright, I have 12 jars that have a similar substrate mix to PF. Bascially the mix was:
2 2/3 cup organic rye flour
8 cups vermiculate
2 2/3 cups water
In one of these cups of water, I put a teaspoon of honey

I sterlized the jars with steam intead of a PC, for around 20-30 mins. Let cool for around 5-6 hours...

I innocted with 1-2 cc's per jar through a single hole big enough for the needle in the top of the jars, and then covered the hole with a piece of packing tape shortly after the inoculation. They have been sitting in my closet in a box around 75-80 degrees for 15 days now.

There is just no growth of any sort in the jar. The only thing that I think is growing is under the clear packing tape, on every jar, there is a patch of white stuff, that I observed over a few days, and it grew....too bad its not growing on the substrate...

I'm thinking of buying a few more syringes and trying the whole thing over with a PC and WBS....any ideas why the ones I have now are not growing and what I can do better in the future to get a more successful grow.

Thanks everyone


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Offlinepuwtrip
spirit molecule

Registered: 03/16/05
Posts: 203
Last seen: 1 year, 5 months
Re: 0 Growth 0 Contaminates [Re: MycoGod]
    #4690409 - 09/21/05 03:40 PM (11 years, 5 months ago)

water moisture content.
jars still hot inside.
not enough oxygen (packing tape over holes).
fluctuating temperature.
inoculation to center of jar (no growth visible yet).


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OfflineMycoGod
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Registered: 09/14/05
Posts: 9
Last seen: 10 years, 5 months
Re: 0 Growth 0 Contaminates [Re: MycoGod]
    #4690417 - 09/21/05 03:42 PM (11 years, 5 months ago)

I also have to add that there is no condensation in the bottom of the jars and on most jars I did hit part between the substrate and glass.. If it makes any differance, I Innocted with Puerto Rican and "Z".


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Offlineiateshaggy
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Re: 0 Growth 0 Contaminates [Re: MycoGod]
    #4690444 - 09/21/05 03:50 PM (11 years, 5 months ago)



--------------------
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I can flash/jtag/repair 360's, pm for details.


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OfflineMark31186
Psychedelic Mind
Registered: 09/14/05
Posts: 37
Last seen: 11 years, 5 months
Re: 0 Growth 0 Contaminates [Re: iateshaggy]
    #4690622 - 09/21/05 04:40 PM (11 years, 5 months ago)

Im guessing it might be not enough air getting to the cake... one hole with it covered in tape? the only O2 would be on top and thats prolly why you only noticed growth on top. Lack of O2 would stunt growth i believe... people have said this about fruiting chambers so im sure the same concept works in jars too. After all we all need air to breath :smile:

GL!


--------------------
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It slips away, all your money won't another minute buy

Dust in the wind, All we are is dust in the wind


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InvisibleFooManM
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Re: 0 Growth 0 Contaminates [Re: Mark31186]
    #4690640 - 09/21/05 04:44 PM (11 years, 5 months ago)

That would slow growth, but you should see SOME growth after 15 days. I'm thinking the problem is either that the spores were no good, the jars were still hot when you inoculated or possibly your rye flower was enriched and not really organic?


--------------------


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OfflineBamaman
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Registered: 08/04/05
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Re: 0 Growth 0 Contaminates [Re: FooMan]
    #4690663 - 09/21/05 04:53 PM (11 years, 5 months ago)

uncover those holes! do you have a verm layer over your sub? if so go ahead and make your lids really loose..."jiggly" as it were.

if you only have one hole per jar then loosening those lids considerably should give you the extra FAE that you need. if you don't have contams then don't give up on those jars!


--------------------
Diabetes causes hamsters.


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Amazon Shop for: ½ Pint Jars, Rye Grain, Vermiculite, Wild Bird Seed

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