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 kurupira
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Registered: 08/29/01
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Last seen: 17 years, 7 months
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 An attempt to extract... some Q's 
#4685773 - 09/20/05 10:51 AM (18 years, 6 months ago) |
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Got this paper flying over the street..
"Some Huautla and Mazatapec fruits were dried, pulverized and combined, weighing 153,5 g. To this powder here was added 1L of Methanol in a 3L flat bottom flask and left undisturbed for 24h. The mixture was vacum filtered and the liquid collected. The extraction process was repeated with 2x 0,5L of fresh solvent, the last one being warmed to boiling point and left the time above. The three extractions were combined, around 1,5L.
A 1mL aliquot of this yellowish liquour was mixed with same volume of chloroform in a test tube and there were a "flufy white" precipitate that settled on the bottom with time. An attempt to vacum filter it over a sintered glass funnel failed, when drying the precipitate, seemed to dissolve/liquify and passed through filter.. (same ocurred when mixed with acetone)."
So some Q's...
-Could the precipitates be just crap/sugars or has it some of the psilo?
-What did dissolve the precipitate, atmospheric humidity?
K.
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ChuangTzu
starvingphysicist
Registered: 09/04/02
Posts: 3,060
Last seen: 10 years, 6 months
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 Re: An attempt to extract... some Q's [Re: kurupira]
#4689105 - 09/21/05 02:25 AM (18 years, 6 months ago) |
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That's basically a repeated PF extraction. Alcohols are known to extract the actives as well as fats, fatty acids, and enzymes---including the enzymes which catalyze the breakdown of the actives.
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kurupira
Hyperspacer
Registered: 08/29/01
Posts: 35
Last seen: 17 years, 7 months
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Re: An attempt to extract... some Q's [Re: ChuangTzu]
#4689139 - 09/21/05 03:17 AM (18 years, 6 months ago) |
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Sure it is, but if one could remove the soluble trash that comes with psilocybin(precipitate it) then one could try crystalize it as it is or as the fumarate salt (don't know if i's possible but worth a try).
I dont' think a (dry)methanol extraction would extract phosphatase enzymes, as reported by Gartz, "Extraction and analysis of indole derivatives from fungal biomass", 1994.
One more request:
Quote from "A Rapid Extraction and GC/MS Methodology for the Identification of Psilocyn in Mushroom/Chocolate Concoctions", Microgram Journal:
"...In a more refined method, the mushrooms are extracted with methanol, and the co-extracted sugars then precipitated with acetone; the resulting solution is concentrated prior to analysis by GC/MS (13)..."
"...13. Timmons JE. Identification of psilocyn and psilocybin using gas chromatography and mass spectrometry. Microgram 1984;17(2):28 31..."
So i woud be VERY satisfied if one get the paper above for a look.
thanks.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: An attempt to extract... some Q's [Re: kurupira]
#4689432 - 09/21/05 08:14 AM (18 years, 6 months ago) |
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My attempts to evaporate the alcohol until I had crystals resulted in either failure or the loss of active components in the crystals after a very short time. Perhaps it's because the actives are ruined by contact with the air, but I really have no idea as I'm not a biologist or chemist.
The best results I obtained were from doing as you did with 190 proof everclear, no acetone. However, once the alcohol was evaporated down to about a shotglass full, I stopped. This is just before crystals form and drop to the bottom of the solution. If you begin to see crystals, stop and add a small amount of fresh everclear to re-disolve them.. Refrigerate the results for indefinite storage. By using a 1/4 lb of dry material, I was able to get 1/2 cup of concentrated solution. One teaspoon is all it takes to do the trick. Add to orange juice or whatever floats your boat.
RR
RR
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
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kurupira
Hyperspacer
Registered: 08/29/01
Posts: 35
Last seen: 17 years, 7 months
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Re: An attempt to extract... some Q's [Re: RogerRabbit]
#4696448 - 09/22/05 05:31 PM (18 years, 6 months ago) |
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my guess is that the crystals are inactive material(mostly sugars)...
if one could isolate it one would peform bioassay...
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ChuangTzu
starvingphysicist
Registered: 09/04/02
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Re: An attempt to extract... some Q's [Re: kurupira]
#4698834 - 09/23/05 12:43 AM (18 years, 6 months ago) |
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There is so [Edit: much] posted about this already....
[Edit: And it's chuang, not chang  ]
Edited by ChuangTzu (09/23/05 01:30 PM)
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kurupira
Hyperspacer
Registered: 08/29/01
Posts: 35
Last seen: 17 years, 7 months
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Re: An attempt to extract... some Q's [Re: ChuangTzu]
#4700366 - 09/23/05 12:05 PM (18 years, 6 months ago) |
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eh... ???
thank you chang...
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