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Amazon Shop for: ½ Pint Jars, Agar, Autoclave, Malt Extract, Paul Stamets

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Offlinebuggers
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agar transfers
    #4671820 - 09/17/05 12:20 AM (11 years, 2 months ago)

i did my first agar transfers earlier today... i'm doing p. caerulipes which were started on antibiotic agar and placed on peroxidated agar.

i put the mycelium side facing down toward the new plate... is this right? or should the mycelium face upwards?

also, how thick do people usually pour their plates... mine were like 3/8" thick and i poured the peroxidated plates that are about the same thickness... should i shoot to make them thinner for my next transfer?

i'm beginning to discover that agar is a lot of fun. its pretty easy and inexpensive to get into and gives me some more mycological toys to play with/look at while jars are colonizing, bins of horse poo are colonizing, caseings are being colonized, and waiting for pins on stuff in the fruiting chamber... i recommend everyone to give it a try!

i've gotten a big kick out of an extra plate that i put some toe jam, a booger, and earwax on... my toejam has many different kinds of nasty shit in it... boogers ended up making a bunch of green mold... and earwax seems to be sterile... good to know!


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Offlinebuggers
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Re: agar transfers [Re: buggers]
    #4671890 - 09/17/05 12:37 AM (11 years, 2 months ago)

come on guys... somebody's gotta think its cool/funny that i cultured my boogers and my toe jam!!


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InvisibleThumpaCap
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Re: agar transfers [Re: buggers]
    #4672155 - 09/17/05 01:33 AM (11 years, 2 months ago)

:thumbup:

that's awsome!!(?)

:shrug:


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OfflineRogerRabbitM
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Re: agar transfers [Re: ThumpaCap]
    #4673145 - 09/17/05 07:45 AM (11 years, 2 months ago)

I place the mycelium up on the new dish. This keeps it exposed to air so it can grow faster. It will crawl off the wedge and onto the new plate with no problem. I also transfer a piece no larger than a grain of rice when making transfers. You can pour plates thinner than you did. I get two sleeves of twenty from one liter of agar solution. You're right, agar is a lot of fun.
RR


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InvisibleRoadkill
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Re: agar transfers [Re: buggers]
    #4673702 - 09/17/05 12:46 PM (11 years, 2 months ago)

face the mycelium upwards...
like Roger said.

I love working with agar too.
Nothing like working with isolates and clones.
:smile:

lmfao @ toe jam, a booger, and earwax!~
you have too much time on your hands.

tc


--------------------
Laterz, Road

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You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinemr_minds_eye
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Re: agar transfers [Re: Roadkill]
    #7587045 - 11/02/07 11:40 AM (9 years, 1 month ago)

Fucking a I wish I had freetime like that!! That is funny though.


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Our quest for discovery fuels our creativity in all fields, not just science. If we reached the end of the line, the human spirit would shrivel and die. But I don't think we will ever stand still: we shall increase in complexity, if not in depth, and shall always be the center on an expanding horizon of possibilities.
-Stephen Hawking


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Invisiblelipa
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Re: agar transfers [Re: buggers]
    #7587097 - 11/02/07 11:57 AM (9 years, 1 month ago)

I place my wedges face down. There is plenty of oxygen available at the time of transfer for the mycelium to use for recovery. Really It is not a big issue, but placing the mycelium in direct contact with the agar ensures faster recovery times in my opinion. Especially when using peroxidated agar. I have cultured both ways and have found through numerous transfers and by keeping a journal that contact is faster but not enough to be worried about.


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OfflineThe shroomy 1
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Re: agar transfers [Re: lipa]
    #7587265 - 11/02/07 12:48 PM (9 years, 1 month ago)

And my question is where do you get antibiotic agar and peroxidated agar? As far as the original question is concerned, Face up works for me. The toe jam, boogers, and earwax experiment was awesome! That is something I would totally do! I just never seem to have that spare plate. LOL!


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AMU Q&A thread.


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InvisibleRoadkill
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Re: agar transfers [Re: The shroomy 1]
    #7587294 - 11/02/07 12:56 PM (9 years, 1 month ago)

Quote:

The shroomy 1 said:

And my question is where do you get antibiotic agar and peroxidated agar?





I think most people make up their own.

I do.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisiblelipa
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Re: agar transfers [Re: The shroomy 1]
    #7587348 - 11/02/07 01:12 PM (9 years, 1 month ago)

If you would like a recipe for my peroxide agar . i would be happy to give it out.


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Offlinekatfish
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Re: agar transfers [Re: lipa]
    #7590018 - 11/03/07 03:15 AM (9 years, 1 month ago)

I have tryptic soy agar it is said for growing bacteria on it could it be used for this as well?


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OfflineRogerRabbitM
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Re: agar transfers [Re: katfish]
    #7590506 - 11/03/07 11:27 AM (9 years, 1 month ago)

No. Use malt extract agar. You don't want to select for bacteria, you want to select for fungi. Gentmycin sulphate is the antibiotic of choice because it can be added pre-sterilization and will survive the autoclave. Use 20 grams per liter.

I believe sporeworks has an antibiotic agar for sale. If not, stamets sells it on his site if you don't want to make your own.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat


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Invisibleorchidfanatic
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Re: agar transfers [Re: RogerRabbit]
    #7590636 - 11/03/07 12:44 PM (9 years, 1 month ago)

I get my agar from mycosupply .. I make 250 ML batches at a time its lasted forever .. I might try the antibiotic agar next time


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Amazon Shop for: ½ Pint Jars, Agar, Autoclave, Malt Extract, Paul Stamets

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