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Offlinevescudero0
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Registered: 01/28/05
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which step requires more sterility??
    #4656428 - 09/13/05 08:12 PM (11 years, 4 months ago)

Does mixing the subs. ( verm & BRF or WBS which ever..)requiere the most???
Does inoculating requieres the most??
Or does casing requiere the most????
right now im in the colonizing step, so at least im there..
im wondering about the caseing sterilty? sice i havnt been there yet-
does this step require the most or what??

first of all, i know that the 3 of these requier good steriltiy
but i was wondering , which one is farther most, maybe especify an order?? please.
thanx


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Offlinedjsage420
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Re: which step requires more sterility?? [Re: vescudero0]
    #4656452 - 09/13/05 08:20 PM (11 years, 4 months ago)

Everything up to inoculation takes the most, my colonizing jars sit on a dusty water heater.
You should practice under semi sterile conditions though, one contam can wipe out everything you have.


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OfflineEquilibriuM
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Re: which step requires more sterility?? [Re: djsage420]
    #4656529 - 09/13/05 08:39 PM (11 years, 4 months ago)

i would have to say casing takes the least.


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Invisiblegema
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Re: which step requires more sterility?? [Re: vescudero0]
    #4656557 - 09/13/05 08:48 PM (11 years, 4 months ago)

i would go with innoculating jars. using a cheap home made glove box works wonders. you dont have to worry about sterilty when mixing subs if you use a pc but remember to properly clean your subs especially wbs.


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OfflineWhiteBunny
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Re: which step requires more sterility?? [Re: gema]
    #4656728 - 09/13/05 09:37 PM (11 years, 4 months ago)

when inoculating your jars is when someone is most susceptible to having contaminants enter your newly sterilized jars. Good luck!


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Edited by WhiteBunny (09/13/05 09:38 PM)


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InvisibleOldSpice
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Re: which step requires more sterility?? [Re: vescudero0]
    #4656936 - 09/13/05 10:30 PM (11 years, 4 months ago)

When jars are fully colonised is when you can be ok as far as contams
You need to be as sterile as possible thru the whole grow


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Offlinespaceman
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Re: which step requires more sterility?? [Re: OldSpice]
    #4657161 - 09/13/05 11:21 PM (11 years, 4 months ago)

I haven't been growing long so all my contams have been by inoculation.


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InvisibleFooManM
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Re: which step requires more sterility?? [Re: EquilibriuM]
    #4657193 - 09/13/05 11:28 PM (11 years, 4 months ago)

Quote:

EquilibriuM said:
i would have to say casing takes the least.




I would say that is crazy! Casing is one of the times that can make or break a grow. Even with sterilized casing soil, you can easily get contams by not being clean when you fan and mist. Keeping the casing too moist will also cause problems.


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Offlinevescudero0
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Re: which step requires more sterility?? [Re: FooMan]
    #4664356 - 09/15/05 12:21 PM (11 years, 4 months ago)

thanx guys very helpfull info...
since i was going to the next step, which was "caseing", thats the one im concerned about now...it seems that i made it thru inoculation so ITS ALL GOOD!!!
now just gotta be carefull with caseing, so ill try my best.,.
if u guys have any helpfull tips about caseing, IM ALL EARS
thanx again...


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InvisibleFooManM
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Re: which step requires more sterility?? [Re: vescudero0]
    #4664394 - 09/15/05 12:32 PM (11 years, 4 months ago)

Check hyphae's posts. He is the casing guy. I think his sig has a good link to his thread about casing. GL dude!


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Offlineblackout
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Re: which step requires more sterility?? [Re: FooMan]
    #4664695 - 09/15/05 01:41 PM (11 years, 4 months ago)

Preparing the sub requires no sterility at all, since it has yet to be sterilized. inoculation requires the most since your substrate is a nice nutritious food ready for any spores that land on it the grow, be they cubensis or mold.

FooManShroom is right in the fact that it can ruin a grow which is upsetting after waiting all the time to colonise and prepare casing.

When colonised the mycelium is strong and can fight off competitor spores that land on it and germinate. You still need to be clean though.

But if you open a colonised jar an uncolonised jar in a room with dirty air for 10 seconds, then put the lid back on I would bet money that the uncolonised jar will show contamination within the week and the colonised jar will not within the week.

And I hate gambling :wink:

I have seen it recommended to use a casing of 8-8.5ph which will help stop green mold.


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Edited by blackout (09/15/05 01:42 PM)


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