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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Rye Grain

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Offlineswingline
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temp change during colonization
    #4655074 - 09/13/05 03:09 PM (11 years, 2 months ago)

my cake are colonizing at about 76 deg. two are about 2 1/2 weeks along and 5 are about 4 days. i am wondering if it would cause problems if i increased the temp to a more ideal 83-85 at this point?
the jars contain the b+ strain. the older jars are about 50% and the younger ones have just germinated.


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Invisiblethegatewaydrug
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Re: temp change during colonization [Re: swingline]
    #4655084 - 09/13/05 03:10 PM (11 years, 2 months ago)

i wouldnt think so... how many degrees different?


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Offlineswingline
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Re: temp change during colonization [Re: thegatewaydrug]
    #4655089 - 09/13/05 03:11 PM (11 years, 2 months ago)

about 8 deg. warmer is what i would like to achieve


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Invisiblethegatewaydrug
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Re: temp change during colonization [Re: swingline]
    #4655101 - 09/13/05 03:12 PM (11 years, 2 months ago)

i wouldnt put it past 82 degrees if i were u, but i guess u could risk it:shrug:


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May God have mercy upon my enemies, because i won't.

General George S. Patton
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OfflinePixie1420
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Re: temp change during colonization [Re: thegatewaydrug]
    #4655218 - 09/13/05 03:40 PM (11 years, 2 months ago)

try shaking your jars, worked wonders for me.


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InvisibleLakefingers

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Re: temp change during colonization *DELETED* [Re: Pixie1420]
    #4655590 - 09/13/05 05:12 PM (11 years, 2 months ago)

Post deleted by Lakefingers

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Offlinealienated
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Re: temp change during colonization [Re: Lakefingers]
    #4655616 - 09/13/05 05:16 PM (11 years, 2 months ago)

What are they on? Rye, or PFTek? If they're PFTek, don't shake (you should even be able to if you preped your jars right)... If you're using Rye, or some kind of berry/kernal/etc... then you can shake.

I incubate at 75-80... anything hotter is almost pointless and not worth the risk (of that nasty green stuff)


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I dont even grow these things... I just post for this guy that knows a guy, who's sisters boyfriend dumped about 2 months ago.


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Offlineswingline
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Re: temp change during colonization [Re: alienated]
    #4655659 - 09/13/05 05:23 PM (11 years, 2 months ago)

i don't plan on shaking them, they are on cooked whole quinoa an brown rice.

i moved them from a closet to the top of the hot water heater (about 84-85 degrees) if the temp goes above 86 i am going to move them back.


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OfflineRogerRabbitM
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Re: temp change during colonization [Re: swingline]
    #4655738 - 09/13/05 05:44 PM (11 years, 2 months ago)

One of these days the myth that an 'air' temp of 86F provides the best growth will go away for good. Until then, people will continue to expose their jars to high temps and wonder why they get moldy or bacterial infected projects.

Thermogenesis causes the internal temperature of your substrate to be up to several degrees warmer than the surrounding air. If your air temp is 85 to 86, your jars are 90+ on the inside, and will be much more likely to contaminate than if the air temp is 75 to 80. If you're comfortable in a room with a t-shirt on, your jars will be happy in the same room. No incubator or other means of heating is necessary.
RR


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Offlineswingline
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Re: temp change during colonization [Re: RogerRabbit]
    #4655870 - 09/13/05 06:11 PM (11 years, 2 months ago)

yeah, i got worried about the temps on the waterheater and moved the jars back to the closet, i figured it worked so far. good thing because i guess my readings were taken with the heater off cause just a minute ago looked and the temp had gone up to 87+.


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Offlinespaceman
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Re: temp change during colonization [Re: swingline]
    #4655943 - 09/13/05 06:26 PM (11 years, 2 months ago)

If you are using grains prepared right and are extremely sterile when you inoculate then there is no reason why you can't keep em at 84 degrees. If you keep them at below 40 or above 160 then you won't get contams. Then again you won't get mycellium eigther. The difference between 75 degrees and 86 degrees is negligible. Bacteria grows just as well T 75 AS IT DOES AT 85. You will risk nothing by keeping them at that temp. Or whatever, just wait longer for your jars to colonize and risk bacterial spores germinating in that extra time and ruining your jars. It's up to you.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Rye Grain

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