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OfflineShroomr
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Registered: 09/04/05
Posts: 16
Last seen: 18 years, 4 months
Just started
    #4645923 - 09/11/05 12:21 PM (18 years, 6 months ago)

Hello all, I just need a little bit of advice just to make sure I'm doing everything right. 8 days ago I incolated 10 jars with a B+ strain syringe using the PF tek. i then put them in my closet in a tub.(which I'm gonna use has the grow chamber later)Its been 8 days and I'm only seeing a little white on one jar. The temp in the tub has been around 75-77 the hole time with around 50% humidity. I'm i doing everything right??? for awhile I thought I didn't be very sterile but I guess so cause there is white on one jar. Its about the size of a dime the white. Anyway does everything sound OK? do they just need more time and thats all? BTW this is my first attempt and any advice or help would be appreciated. Thanks in advance.

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OfflineJabberw0cky
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Registered: 08/01/05
Posts: 158
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Re: Just started [Re: Shroomr]
    #4645937 - 09/11/05 12:24 PM (18 years, 6 months ago)

You know what I think? I think B+ is a slow colonizer. That's why I'd recommend Golden Teachers to newbies rather than B+'s.
Anyway, you should put like a heating blanket under the tub. And keep a thermostat in there. It should be around 85 degrees. But I like to keep mine at 80 degrees. But mine are slowly, but surely colonizing. I have two of them that are about to touch the other myc injection site on the jar after about a little over two weeks after injection.
-pokes at his jar-
Patience is a virtue.
-Jabber


--------------------
"Beware the Jabberwock, my son!
The jaws that bite, the claws that catch!
Beware the Jubjub bird, and shun
The frumious Bandersnatch!"
He took his vorpal sword in hand:
Long time the manxome foe he sought --
So rested he by the Tumtum tree,
And stood awhile in thought.
Once upon a looking glass...

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InvisibleSmartweed
Psychelica
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Registered: 06/08/05
Posts: 1,033
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Re: Just started [Re: Shroomr]
    #4645944 - 09/11/05 12:25 PM (18 years, 6 months ago)

You might want to raise the temp in your incubator to about 82-86. Also are the holes in your caps covered or uncovered? I noticed my jars colonized faster when they were uncovered.

If you used the correct water in the mix, humidity isnt really a huge factor during the incubation period.


--------------------
I believe that if people would learn to use LSD's vision-inducing capability more wisely, under suitable conditions, in medical practice and in conjunction with meditation, then in the future this problem child could become a wonderchild. - HOFFMAN

My Print/Syringe Trade List

Edited by Smartweed (09/11/05 12:26 PM)

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OfflineShroomInduced
I read too manymycology books
Male

Registered: 04/19/05
Posts: 309
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Re: Just started [Re: Smartweed]
    #4645971 - 09/11/05 12:31 PM (18 years, 6 months ago)

yeah, my optimal tempature is 83 in the incubation chamber. your temps are too low, make sure you have the lids on loose, not tight. They need the air exchange.


--------------------
That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love.

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OfflineOriginal
God, I lovethis...
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Registered: 08/16/05
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Re: Just started [Re: Smartweed]
    #4645981 - 09/11/05 12:34 PM (18 years, 6 months ago)

Temperatures should be 80-84 deg. F.

You don't need humidity for colonizing, only for fruiting.

Newcomers commonly make that mistake ^^^^


And I'm tripping :P


Oh and keep the holes uncovered..

http://www.shroomery.org/forums/showflat...1&fpart=all

:smile:


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-------------------------------------------------------------------------------
Colonized cakes smell like South Florida...

Seriously...
---------------------------------
I can't sleep cuz the mexicans keep lighting their fireworks...
---------------------------------
If ya don't want trouble, ya gots ta crumble...

-Johnny Cochran on the use of crumbled vs. whole cakes..
-------------------------------------------------------------------------------

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OfflineShroomr
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Re: Just started [Re: Shroomr]
    #4646108 - 09/11/05 01:21 PM (18 years, 6 months ago)

ok so i need to put a heating pad under the tub and i have the top covered with foil ill take it off now then other that everything seems ok? thanks for your help

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Offlinespaceman
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Re: Just started [Re: Original]
    #4646121 - 09/11/05 01:27 PM (18 years, 6 months ago)

I think colonization times depends on what it is colonizing. I am colonizing B+ and GT right now, both on an LC and on rye. The B+ is tearing up the LC but not growing too fast in the rye. My GT's are just the opposite. My teks were followed to a t and all are in the same incubaton chamber at 85 degrees.

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Offlinespaceman
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Re: Just started [Re: spaceman]
    #4646124 - 09/11/05 01:29 PM (18 years, 6 months ago)

that sounds good. GL

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OfflineShroomr
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Re: Just started [Re: Shroomr]
    #4646169 - 09/11/05 01:40 PM (18 years, 6 months ago)

WOW i took off the foil and there is white on most of the jars i just couldnt see because of the foil

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OfflineShroomInduced
I read too manymycology books
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Registered: 04/19/05
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Re: Just started [Re: Shroomr]
    #4646185 - 09/11/05 01:43 PM (18 years, 6 months ago)

YEah id leave them uncovered, leave the lids on loose.


--------------------
That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love.

Computer Fan Tek
Vote for you favorite strain of cubes 97 choices!

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OfflineShroomr
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Last seen: 18 years, 4 months
Re: Just started [Re: Shroomr]
    #4646226 - 09/11/05 01:53 PM (18 years, 6 months ago)

i should loosen the lids? there is no way to get contams that way is there? i also took the lid off the tub and the heating pad is under there its 80 in there now whats the max themp i should have?

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Offlinespaceman
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Re: Just started [Re: Shroomr]
    #4646240 - 09/11/05 01:57 PM (18 years, 6 months ago)

The dry vermiculite layer that you put in there (right?) acts as a conamination barrier...

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OfflineShroomInduced
I read too manymycology books
Male

Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 12 years, 7 months
Re: Just started [Re: spaceman]
    #4646252 - 09/11/05 02:00 PM (18 years, 6 months ago)

106 is thermal death of mushies, you want your temp to be between 80-86 i found that mine like 83 in the chamber. (in otherwords the air tempature in the incubation chamber should be 83) dont forget the jars are always a few degrees warmer inside. The reason you leave the lids on loose is so that air can get in and out. If there is not sufficent gas exchange it will slow grow severly, and it will promote anerobic bacteria.


--------------------
That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love.

Computer Fan Tek
Vote for you favorite strain of cubes 97 choices!

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OfflineShroomr
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Registered: 09/04/05
Posts: 16
Last seen: 18 years, 4 months
Re: Just started [Re: Shroomr]
    #4646267 - 09/11/05 02:02 PM (18 years, 6 months ago)

ok im gonna loosen the tops then and yeah i put the half inch layer of dry verm on

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OfflineShroomInduced
I read too manymycology books
Male

Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 12 years, 7 months
Re: Just started [Re: Shroomr]
    #4646283 - 09/11/05 02:06 PM (18 years, 6 months ago)

Then you should be a ok. Ill give you another tip. Next time you make jars, poke some quarter inch holes intop and stuff them with poly fill. I do that and leave the lids on loose, i can go from inaculation to a fully colonized jar in 8 days.


--------------------
That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love.

Computer Fan Tek
Vote for you favorite strain of cubes 97 choices!

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OfflineShroomr
Stranger
Registered: 09/04/05
Posts: 16
Last seen: 18 years, 4 months
Re: Just started [Re: Shroomr]
    #4646345 - 09/11/05 02:23 PM (18 years, 6 months ago)

thanks for the tip man

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Offlineweenrules
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Registered: 09/11/05
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Re: Just started [Re: Shroomr]
    #4646560 - 09/11/05 03:21 PM (18 years, 6 months ago)

shit. iv had my lids on tight for the past three days.. the basic cubensis growing technique i read on here did not say to leave the lids loose:( will it be ok now that i'v loosened them??

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OfflineShroomr
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Registered: 09/04/05
Posts: 16
Last seen: 18 years, 4 months
Re: Just started [Re: Shroomr]
    #4646611 - 09/11/05 03:43 PM (18 years, 6 months ago)

my were on tight for 8 days and i saw white on 8 of 10 jars just made them loose today

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