Home | Community | Message Board


Gaiana.nl
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars

Jump to first unread post. Pages: 1
OfflineDingus
Stranger
Registered: 08/28/05
Posts: 13
Last seen: 9 years, 5 months
PF tek1 - cloning mod
    #4645350 - 09/11/05 11:38 AM (11 years, 3 months ago)

I wrote a couple weeks ago about modifying the PF tek for cloning as an academic experiment. My setup was identical to the PF tek, however I needed a system to insert tissue specimens in a sterile way. My solution was to add pipes through the lid; i used small ones, 1/16" OD, 1/48" ID, four, extending through the lid just as a syringe owuld do. I epoxied them in place before sterilization.

anyway, i steam sterilized and then inserted the tissues samples by flaming the ends of the tubes, putting a small piece of tissue on top, and then poking it through the tube into the substrate with a piece of flamed wire.

I did 4 jars, added tissue to all but one as a control jar. its been about 10 days now, and suprisingly, its working! only 2 of the jars show growth, and in each jar from only one of the tissue sites.

My question is; will it still work if only one of the 4 tissues sites are growing? right now they look almost identical to the photos in the PF Tek guide, showing the foggy whiteness spreading out from the point of inoculation. can this fruit, or do you need two different strains in the same jar to performa some kind of sexual reaction?

ive got pics, this is destinated to go on my website.

oh, i should add im growing button mushrooms, nothing psychadelic; im interested in mushrooms and like a challenege.

EDIT: I should also add that i was concerned about contams via the open tubes, and although I had planned on simply crimping them shut (theyre aluminum), i did not. i just put some packing tape over then and it seems fine, i think the reynolds number is so high for a tube that narrow and long that nothing really flows into them. thus far no contams, even in the control jar.


Edited by Dingus (09/11/05 11:43 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* PF Classic Clone theratatat 650 4 07/06/07 12:12 PM
by euphoricpoison
* PF's peroxide cloning tek rygo796 1,450 4 12/08/06 12:42 PM
by RogerRabbit
* Faht's oven bag clone tek
( 1 2 all )
fahtster 28,040 27 01/22/15 01:19 AM
by tomcat
* cloning question High_On_Magic 1,349 8 10/29/03 03:19 PM
by Seraph
* First clone (F+)
( 1 2 all )
Boris 6,453 38 01/27/11 11:01 AM
by Barakanaten
* First grow. Who says PF Tek sucks? PF PORN -- (UPDATE 11/19: 2nd harvest ~40g wet)
( 1 2 all )
Alprazolom 28,358 34 08/11/09 01:07 AM
by Lostkeys
* Glovebox is royally F@#$% - Epoxy Resin... HELP!
( 1 2 all )
Mobius_Strip 1,827 20 07/21/05 04:21 PM
by Hotnuts
* NEW PF TEK: How to alter the dna of mushrooms!
( 1 2 all )
Jammer 3,713 23 02/20/02 01:56 AM
by Jammer

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
251 topic views. 22 members, 150 guests and 29 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Mushrooms.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.006 seconds on 13 queries.