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Registered: 08/28/05
Posts: 13
Last seen: 10 years, 26 days
PF tek1 - cloning mod
    #4645350 - 09/11/05 11:38 AM (11 years, 10 months ago)

I wrote a couple weeks ago about modifying the PF tek for cloning as an academic experiment. My setup was identical to the PF tek, however I needed a system to insert tissue specimens in a sterile way. My solution was to add pipes through the lid; i used small ones, 1/16" OD, 1/48" ID, four, extending through the lid just as a syringe owuld do. I epoxied them in place before sterilization.

anyway, i steam sterilized and then inserted the tissues samples by flaming the ends of the tubes, putting a small piece of tissue on top, and then poking it through the tube into the substrate with a piece of flamed wire.

I did 4 jars, added tissue to all but one as a control jar. its been about 10 days now, and suprisingly, its working! only 2 of the jars show growth, and in each jar from only one of the tissue sites.

My question is; will it still work if only one of the 4 tissues sites are growing? right now they look almost identical to the photos in the PF Tek guide, showing the foggy whiteness spreading out from the point of inoculation. can this fruit, or do you need two different strains in the same jar to performa some kind of sexual reaction?

ive got pics, this is destinated to go on my website.

oh, i should add im growing button mushrooms, nothing psychadelic; im interested in mushrooms and like a challenege.

EDIT: I should also add that i was concerned about contams via the open tubes, and although I had planned on simply crimping them shut (theyre aluminum), i did not. i just put some packing tape over then and it seems fine, i think the reynolds number is so high for a tube that narrow and long that nothing really flows into them. thus far no contams, even in the control jar.

Edited by Dingus (09/11/05 11:43 AM)

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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