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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 9 years, 6 months
Needing advice
    #4642677 - 09/10/05 05:37 PM (11 years, 5 months ago)

I was directed here after posting in "The Pub". The mod down there said you guys were great. I was really hoping to find a cultivator in Canada so that we could talk on more common ground. But soon realized the situation I had put myself in as far as legalities go. Anyways...

I registered a while ago, have made a few posts, asked a few questions, but generally just done alot of lurking on the boards.

Now to my problem. I've attempted mushroom cultivation using the pf-tek and the b+ strain three times now with absolutely no results whatsoever. Now I must explain, I'm not an inept person, and funny as it seems, this just isnt workin!

I've narrowed it down to possibly bunk spores, with the help of some fellow members in my last posts. I'm aware that shroomery mods arent supposed to be reccomending spore suppliers that dont sponsor the site, but, Ive ordered from just about every supplier that will ship to Canada and the results are the same each time. I've even looked around town, trying to find another cultivator I could possibly purchase spores from with no luck.

I'm coming to the end of my rope with this, I can't stand to have a bunch of bloody fungus defeat me. I was also told that hook-up posts are not allowed, but if anyone would like chat through PM it would be greatly appreciated.

Suggestions, flames, comments, tips, opinions, anything helps.


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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Offlinelemon_lw
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Registered: 10/18/04
Posts: 3,622
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Last seen: 9 years, 5 months
Re: Needing advice [Re: kranked]
    #4642709 - 09/10/05 05:43 PM (11 years, 5 months ago)

okay a few questions.
what tek are you using
if the pf tek (ill go ahead and assume your using it) what is your mixture ratio.
what is your incubation temps
how much spore solution did you use
did you have tape on the jars
did you let your jars cool before you inoculated them


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 9 years, 6 months
Re: Needing advice [Re: lemon_lw]
    #4642725 - 09/10/05 05:46 PM (11 years, 5 months ago)

Yes, the pf-tek
Dont have an exact number on temps, room temperature?
About 1cc per jar.
Yes I taped the holes in the lids.
Yes they cooled.

Its really baffling because I cant even get them to contam. even intentionally.


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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InvisibleOldSpice
Geritol Breath...
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Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Needing advice [Re: kranked]
    #4642735 - 09/10/05 05:50 PM (11 years, 5 months ago)

www.ralphstersspores.com is the best place to start :thumbup:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Offlinelemon_lw
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Re: Needing advice [Re: kranked]
    #4642738 - 09/10/05 05:51 PM (11 years, 5 months ago)

i still need your ratio of mixture for the jars but a few of your things are off
temps of 84-86F is best
you need more spore solution its .5 cc to 1 cc per hole, so that means you should put 2-4 cc per jar
take the tape off after sterilizing the jars


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 9 years, 6 months
Re: Needing advice [Re: lemon_lw]
    #4642753 - 09/10/05 05:54 PM (11 years, 5 months ago)

I found the pf-tek on this site. I cant really remember what the ratios were like. Can you reccomend a good tek?


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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InvisibleOldSpice
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Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Needing advice [Re: lemon_lw]
    #4642761 - 09/10/05 05:57 PM (11 years, 5 months ago)

Quote:

lemon_lw said:
you need more spore solution its .5 cc to 1 cc per hole, so that means you should put 2-4 cc per jar




Thats bullshit all you need is .25cc per hole or a total of 1cc


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Offlinespaceman
mushroom head
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Registered: 07/09/05
Posts: 169
Last seen: 11 years, 5 months
Re: Needing advice [Re: OldSpice]
    #4642769 - 09/10/05 05:59 PM (11 years, 5 months ago)

2/3 c vermiculite, 1/3 brf, 1/3 c water per jar


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InvisibleColonel Kurtz Ph.D
What What?
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Registered: 07/22/04
Posts: 11,113
Loc: Shadow Moses
Re: Needing advice [Re: OldSpice]
    #4642772 - 09/10/05 06:00 PM (11 years, 5 months ago)

Quote:

Alounacara said:
Quote:

lemon_lw said:
you need more spore solution its .5 cc to 1 cc per hole, so that means you should put 2-4 cc per jar




Thats bullshit all you need is .25cc per hole or a total of 1cc




Yeah, 1cc per jar is where it's at.


--------------------
:whatwhat:

There's no better way to rock out than with your cock out!!


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Offlinei8boomers
Title?! neverhad one

Registered: 09/07/05
Posts: 11
Loc: USA
Last seen: 11 years, 4 months
Re: Needing advice [Re: OldSpice]
    #4642779 - 09/10/05 06:02 PM (11 years, 5 months ago)

Ok newbie with first post....love this site.

Maybe you're having the same problem I had where my jars stalled. Do a post search for Taping holes by 'hyphae' who states that the holes should NOT be taped after inoculation where as many of the teks do.

Just my first two cents....


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Offlinelemon_lw
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Registered: 10/18/04
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Last seen: 9 years, 5 months
Re: Needing advice [Re: OldSpice]
    #4642780 - 09/10/05 06:02 PM (11 years, 5 months ago)

Quote:

Alounacara said:
Quote:

lemon_lw said:
you need more spore solution its .5 cc to 1 cc per hole, so that means you should put 2-4 cc per jar




Thats bullshit all you need is .25cc per hole or a total of 1cc




oops yeah i remembered the numbers wrong for a sec, my bad.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Needing advice [Re: spaceman]
    #4642786 - 09/10/05 06:02 PM (11 years, 5 months ago)

Thats an old formula you need 1/4 cup BRF and 1/4 cup water {i use 55cc water} and 1/2 cup verm


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Offlinepuwtrip
spirit molecule

Registered: 03/16/05
Posts: 203
Last seen: 1 year, 5 months
Re: Needing advice [Re: kranked]
    #4642807 - 09/10/05 06:07 PM (11 years, 5 months ago)

kranked, since you say you can't even get the jar to contam, there might be something wrong with your ingredients (though i don't know to what lengths you've gone).

you have a few options but this is what i'd do.

1) try to get a liquid culture going. this requires little effort and supplies and will let you know if the spores are able to produce.

2) try a straight wheat berry(grain) substrate. this what i prefer to use because its easy to drain and moisture levels are easy to attain without measuring. wash wheat a few times, changing the water. soak wheat for 18 hours. put into pot and simmer for 30 minnutes. drain for 20 minutes. load into jars and pc. get an empty syringe you have and pc it as well.

3) use sterile syringe to suck up lc.inoculate jars and wait for growth


if step #1 fails, you know you have defective spores.

if step #2 fails. you need to work on your technique. or buy a grain/rye baggie or premade jars from an online vendor.



Edited by puwtrip (09/10/05 06:13 PM)


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Offlinespaceman
mushroom head
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Registered: 07/09/05
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Re: Needing advice [Re: puwtrip]
    #4643621 - 09/10/05 10:37 PM (11 years, 5 months ago)

Still the ratio is the same 2:1:1.vermiculite, brown rice flour, water


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