Home | Community | Message Board



Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Rye Grain

Jump to first unread post. Pages: 1
InvisibleColonel Kurtz Ph.D
What What?
Male User Gallery
Registered: 07/22/04
Posts: 11,113
Loc: Shadow Moses
Proof that more spores=faster colonization?
    #4642543 - 09/10/05 04:52 PM (11 years, 5 months ago)

So i was goingo to grow Malabar for aesthetic reasons and I got curious for other strains so I also made a syringe of Mazapatec and innoc'd the same amount of solution and the same day both strains to rye.
Both strains are growing under the same exact conditions and I'm replicating this experiment with Thai Koh Samui and it looks like it's going to confirm this experience so far.

Here are the syringes:

Left Mazapatec, right Malabar.

Now, 6 days later, the microboxes look like this:

Malabar:


Mazapatec:


Confirmed? I wouldn't dare to say it, but it' enough of a proof to me :laugh:

:mushroom2: :sun: :mushroom2:

Edit: Title.


--------------------
:whatwhat:

There's no better way to rock out than with your cock out!!


Edited by Deepman (09/10/05 05:25 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTriad
♫Tool♫
Male

Folding@home Statistics
Registered: 08/19/05
Posts: 360
Loc: Canada
Last seen: 6 years, 9 months
Re: Definitive proof that more spores=faster colonization? [Re: Colonel Kurtz Ph.D]
    #4642561 - 09/10/05 04:59 PM (11 years, 5 months ago)

A better experiment would be using 2 of the same strain, I'm not 100% sure but I hear people talking about different strains having different colonization times.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineshafty
fungus is urfriend
Male
Registered: 05/06/05
Posts: 78
Last seen: 9 years, 5 months
Re: Definitive proof that more spores=faster colonization? [Re: Colonel Kurtz Ph.D]
    #4642569 - 09/10/05 05:01 PM (11 years, 5 months ago)

looks like a pretty big difference. one could say that there are differences in the strains and one(the mazapatec) is heartier and a better strain. i say repeat the experiment but with the same strain in both jars. i'd like to add that i've noticed a difference for some jars that i've put more spore solution into, but not always.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesexymoreno
Stranger

Registered: 08/04/05
Posts: 169
Last seen: 10 years, 10 months
Re: Definitive proof that more spores=faster colonization? [Re: Triad]
    #4642577 - 09/10/05 05:05 PM (11 years, 5 months ago)

ya, ditto what the guy on the top said. haha
man, you should know about controlled, manipulated and responding variables in experiments.
in this experiment the controlled variable should have been the strains,
therefore you should've used the same strain,
the manipulated variable was the amount of spores in each syringe and the
responding variable would be the amount of myc growth in 6 days.

next time try this and you'll get more accurate results :smile:
always know which variables are controlled, manipulated or responding,
then you'll have a better understanding of your experiment.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 1 month
Trusted Cultivator
Re: Definitive proof that more spores=faster colonization? [Re: Colonel Kurtz Ph.D]
    #4642623 - 09/10/05 05:22 PM (11 years, 5 months ago)

You call that definitive proof? Wow. Its not the same strain..hell even the same strain, the syringes should come not even from the same print, but from the same water with the same print mixed in. You got to many variables, sorry buddy, dont mean shit.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleColonel Kurtz Ph.D
What What?
Male User Gallery
Registered: 07/22/04
Posts: 11,113
Loc: Shadow Moses
Re: Definitive proof that more spores=faster colonization? [Re: sexymoreno]
    #4642627 - 09/10/05 05:24 PM (11 years, 5 months ago)

Yeah, I know about all of that, but I couldn't 'coz I didn't want to risk it making a few syringes with different solutes from only one print.

Anyway, for those who say the main difference is about strain I beg to differ; the mazapatec is 90% colonized and the alabar less than 5%.
All the other variables are the same, except strain and spore solution.
According to most sources the mazapatec is a slightly faster colonizer, but that's too much of a difference; the malabar should go 2 days slower tops.

On my experiment with TKS I've noticed the same pattern, seeing a lot of colonization points in just two days, while the problem with the malabar is not speed of growth but rather that it only has a few  colonization points, pointing towards lack of spores mating, and so the conclusion is (as far as I'm concerned) that the amount of colonization points are in direct relationship with the amount of spores in the solution, thus providing faster colonization with heavy loaded syringes.

Anyway, this is just common sense and I just wanted to give pictorial "proof". If you don't think it's a valid experiment just discard it all together  :smirk:


--------------------
:whatwhat:

There's no better way to rock out than with your cock out!!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepuwtrip
spirit molecule

Registered: 03/16/05
Posts: 203
Last seen: 1 year, 5 months
Re: Definitive proof that more spores=faster colonization? [Re: sexymoreno]
    #4642662 - 09/10/05 05:34 PM (11 years, 5 months ago)

what the above people said is very important to your experiment. how can you compare differing strains? not only do they have different genetic growth rates, but growth might be affected by temperature and humidty and other conditions.

then, the question of inoculation. how many points did you have and how exactly did you go about it.

for example, lets say you have 2 holes on opposite sides. did you just inject straight down the sides, or did you squirt around all over? did you follow the same procedure for both jars?

then you should also consider if most people use the whole spore syringe? why not stick to 1cc per inoculation point as most people do.

if the idea is to get the fastest mycelial growth possible using spores, then yes, the more you have over more surface area, the merrier. but then you also have to consider fruiting potential between the competing substrains. would the yield be comparable to a mostly singularly dominating substrain?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 1 month
Trusted Cultivator
Re: Definitive proof that more spores=faster colonization? [Re: puwtrip]
    #4642673 - 09/10/05 05:36 PM (11 years, 5 months ago)

Bullshit, I've had strains do that exact same thing...sorry, it aint proof.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Definitive proof that more spores=faster colonization? [Re: Colonel Kurtz Ph.D]
    #4642684 - 09/10/05 05:38 PM (11 years, 5 months ago)

**points at you  :rofl2:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleColonel Kurtz Ph.D
What What?
Male User Gallery
Registered: 07/22/04
Posts: 11,113
Loc: Shadow Moses
Re: Definitive proof that more spores=faster colonization? [Re: puwtrip]
    #4642751 - 09/10/05 05:53 PM (11 years, 5 months ago)

The parameters I haven't mentioned like humidity, substrate, points of inoculation (the solution was squirted and the substrate then shaked) etc are exactly the same in both cases.

Quote:

then you should also consider if most people use the whole spore syringe? why not stick to 1cc per inoculation point as most people do.




Obviously I didn't use the whole syringe in every microbox :smirk: I used the standard method, 1cc per box.

Quote:

but then you also have to consider fruiting potential between the competing substrains. would the yield be comparable to a mostly singularly dominating substrain?




Most probably not, but I can't tell for sure :shrug: I just had two syringes with totally different amounts of spores on them so I got curious as to if the difference would be that big or not.
My intention is to select the best Malabar specimens to develop the strain for aesthetic reasons, just for my enjoyment.

Quote:

if the idea is to get the fastest mycelial growth possible using spores, then yes, the more you have over more surface area, the merrier




That's what I was trying to explain; maybe the experiment is not that much of an objective proof for some of you, but I just thought I'd share :undecided:


--------------------
:whatwhat:

There's no better way to rock out than with your cock out!!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblesrgtm1a
Stranger
 User Gallery
Registered: 02/19/05
Posts: 2,625
Re: Definitive proof that more spores=faster colonization? [Re: scatmanrav]
    #4642790 - 09/10/05 06:03 PM (11 years, 5 months ago)

Quote:

scatmanrav said:
You call that definitive proof? Wow. Its not the same strain..hell even the same strain, the syringes should come not even from the same print, but from the same water with the same print mixed in. You got to many variables, sorry buddy, dont mean shit.





:thumbup: :thumbup: :thumbup: :thumbup:

That doesn't prove a damn thing.  The least you should have done is use the same strain....even then, there are a TON of things left out of that experiment.

I hope that isn't    "enough of a proof to me ".  That's how misinformation starts.

Hats off to ya for trying to experiment though...I like to see that.


Edited by srgtm1a (09/10/05 06:11 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 5 years, 9 months
Trusted Cultivator
Re: Definitive proof that more spores=faster colonization? [Re: Colonel Kurtz Ph.D]
    #4643000 - 09/10/05 07:23 PM (11 years, 5 months ago)

Regardless of anything more spores equal more points of germination which we all know means faster colonization but the drawback here is a jar loaded to the gills with diversity which is opposite of what we want as far as yields are concerned thats why we ISOLATE and/or CLONE this is where we want results, results that count anyway!


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMagashM
Da Bud Guru
 User Gallery

Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: Proof that more spores=faster colonization? [Re: Colonel Kurtz Ph.D]
    #4644291 - 09/11/05 01:42 AM (11 years, 5 months ago)

You know I guess I'm one of the ones that has to say more spores IS better. I make my syringes as dark as fuck or is this dark enough and everybody bitches "what a waste of spores " yadda yadda yadda. I know this, I use quart jars and they are done in 9 days, those not done by 14 days are chucked. "Why Magash don't you do LC?" I have and have a tek in the faq but I ended up hating growing the same strain more then one time. I like to do a different one each grow.

So these are multi spore and only one quart of substrate each casing B+ Haw SA OI Mal

I do agree that LC is better buuuuuuuuuuut I do feel that multi spore will get ya by just fine if needed.  :grin: :wink: :evil: :wexican:


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Proof that more spores=faster colonization? [Re: Magash]
    #4644438 - 09/11/05 02:24 AM (11 years, 5 months ago)

Fuck the more less spore battle i wanna see some of that fine ass reefer you be growing, id like some links where i can buy the right supplements also
Ive been spoiled with hydro blueberry lately and i know i can grow better for me :cool:
Searching searching :stoned:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMagashM
Da Bud Guru
 User Gallery

Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: Proof that more spores=faster colonization? [Re: OldSpice]
    #4644468 - 09/11/05 02:33 AM (11 years, 5 months ago)

Me grow, well I never


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShroomInduced
I read too manymycology books
Male

Registered: 04/19/05
Posts: 309
Loc: A giant mushroom in my he...
Last seen: 5 years, 6 months
Re: Proof that more spores=faster colonization? [Re: OldSpice]
    #4644499 - 09/11/05 02:41 AM (11 years, 5 months ago)

Do you know the definition of experiment. YOU DID NOT DO AN EXPERIMENT, YOU DID A RETARD lol. Anyway though, isn't it obvious that more spore would colonize faster. every spore that goes in produces a hyphae that makes up the mycelial network. The more hyphae that you have means the quicker the network will be established. The other bad miscalculation of your "experiment" is that how do you know the spores from one syringe were not mostly dikaryons? If you want faster colonizing jars, weed out the monokaryons spores.


--------------------
That's a deep kiss too, like the Europeans. You know, the French, they have to unhinge their jaw to show love.

Computer Fan Tek
Vote for you favorite strain of cubes 97 choices!


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Proof that more spores=faster colonization? [Re: Magash]
    #4644508 - 09/11/05 02:46 AM (11 years, 5 months ago)

Shit dude ive got some blueberry left but i cant smoke it
Im doing a drug test monday morning so i can work in Afghanistan
Ck this link at mycotopia
http://forums.mycotopia.net/showthread.php?t=6712
Ill have internet access at the internet cafes
Ill also be taking spore prints with me
Im gonna miss my Shroomery friends a lot
I might need some seedage and sporeage from you guys :smirk:
Its not a soild deal yet but im pretty shure its a positive thing


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMagashM
Da Bud Guru
 User Gallery

Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: Proof that more spores=faster colonization? [Re: OldSpice]
    #4644528 - 09/11/05 02:51 AM (11 years, 5 months ago)

Ha ha ha ha ha ha ha    a drug test to work in Afghanistan the drug kingdom  :tongue:


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* More spores = Faster colonization ? Nephlyte 3,152 13 12/27/05 06:05 PM
by BigGirlLover
* More spores faster colonization? Fizikz 862 7 01/30/09 04:33 PM
by German Kahuna
* More Spores=Faster Colonization ??????? *DELETED* PsyllyMe 1,249 19 08/01/04 11:48 PM
by Brokage420
* Faster colonization method... Grapefruity 763 4 09/24/03 04:10 AM
by Hanky
* Re: longer syringes = faster colonization? Humidity 506 3 04/23/00 04:44 AM
by vivid
* Flipping jars for faster colonization. Experiment (PICS)!!!
( 1 2 all )
ski 5,406 22 09/20/07 11:09 AM
by figgusfiddus
* Adding Coir to HUGE Grain Spawn Bags for Faster Colonization?? Pics comming soon koopa_troopa 4,546 17 04/14/13 08:02 PM
by hauserss
* more spawn = more mushies, or just faster colonize Visigoth 757 3 11/06/02 12:31 AM
by StS

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart, Tmethyl
2,261 topic views. 22 members, 109 guests and 12 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Avalon Magic Plants
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.099 seconds spending 0.005 seconds on 14 queries.