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Offlineredood
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A new spore syringe method hopefully correct me if i'm wrong. (Edited)
    #4635351 - 09/08/05 09:50 PM (12 years, 14 days ago)

Ok since most cakes are made 12 oz jars. I have thought up a way to make cakes and syringes much easier if it has not already been done. Ok first of all you will need one of your sterilized jars you make your cakes in. After cutting one of you fresh shrooms at the very top of your cap making sure not to touch it and then placing the cap directly in the jar sealing it airtight. After 1 to 2 days remove the cap from the jar. It would probably be best if you used a glove box while removing the mushroom at this point. You could either just cap the jar completely airtight or using a syringe of sterilized water inject the water directly into the jar. Then later if you want you can use the jars with dry spore prints to make cakes using that jar. Not to sure how to go about preparing the substrate making it sterile before introducing it to the spore printed jar. But maybe with the help of others here we could make this completely foolproof with 0 chance of contamination. Please feel free to comment or add to any of this if it would work at all i am def gonna have test a bit.


Edited by redood (09/08/05 10:08 PM)


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InvisibleFungusMan
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: redood]
    #4635361 - 09/08/05 09:54 PM (12 years, 14 days ago)

WTF did u say? u used 2 periods in that whole post.


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Offlineredood
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: FungusMan]
    #4635391 - 09/08/05 10:00 PM (12 years, 14 days ago)

excuse my grammer i don't like periods as any man would says anyways it's just more to type lol


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Offlinedjsage420
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: FungusMan]
    #4635402 - 09/08/05 10:02 PM (12 years, 14 days ago)

This has been done, this is how I make my syringes.


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InvisibleOldSpice
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: FungusMan]
    #4635404 - 09/08/05 10:02 PM (12 years, 14 days ago)

Quote:

FungusMan said:
WTF did u say? u used 2 periods in that whole post.



Im not gonna read that shit


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Offlineredood
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: OldSpice]
    #4635418 - 09/08/05 10:06 PM (12 years, 14 days ago)

you would hate to see my handwriting then it really sucks lols I was born brilliant but lazy lol


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OfflineESanceOfCyan
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: redood]
    #4635441 - 09/08/05 10:08 PM (12 years, 14 days ago)

Wow its amazing that we had the same ideas.
I think if you sterilize the jar, put the cap in, two days later take it out, you would have just as sterile of a print as any other way, with a few bonuses. One, put the needle through an inoculation hole and inject sterile water right into it, then pull it out(making a spore syringe).Two, I think if you pre-sterilize your substrate and use sterile techniques you could just shoot a little water trough the hole, rehydrate the spores, then put in the substrate, sterile as possible. Me and my friend were discussing this a few days ago, and the only drawback we could think of was the cost, jars are expensive


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Offlineredood
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: ESanceOfCyan]
    #4635452 - 09/08/05 10:13 PM (12 years, 14 days ago)

Exactly what i was thinking but i am also trying to get feedback from the community on how introduce the sterile substrate directly to the jars bringing contamination factors down to zero hopefully.


Edited by redood (09/08/05 10:22 PM)


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Offlinedjsage420
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: redood]
    #4635490 - 09/08/05 10:23 PM (12 years, 14 days ago)

The only thing is you have to dry the spores after you take the cap out, I usually let it sit for 2 days or the spores stick to the bottom of jars.


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Offlinenunciate
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: ESanceOfCyan]
    #4635502 - 09/08/05 10:26 PM (12 years, 14 days ago)

Making a print in a sterile jar is actually part of the PF Tek.

I wuold think putting substrate into a jar is gonna open a huge window for contams and probably be a pain in the ass. Much more so than just using a syringe.


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Offlinewaixingren
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: redood]
    #4635503 - 09/08/05 10:27 PM (12 years, 14 days ago)

making syringes that way is fine, but making cakes that way would be a bad idea. thas way too many spores for a single cake, it would create too many little colonies of mycelium which would fight and delay the colonization time.


--waixingren


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Offlineredood
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: djsage420]
    #4635515 - 09/08/05 10:31 PM (12 years, 14 days ago)

Quote:

djsage420 said:
The only thing is you have to dry the spores after you take the cap out, I usually let it sit for 2 days or the spores stick to the bottom of jars.




Do you also add substrate to the jar that has the print in it or do you just inject the correct amount of water could you explain?
I know for sure that it is already a proven method for spore prints but i am just trying to innovate the method to reduce the risk factors of contamination


Edited by redood (09/08/05 10:31 PM)


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Offlinedjsage420
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: waixingren]
    #4635519 - 09/08/05 10:32 PM (12 years, 14 days ago)

I agree I only make syringes that way I even put self healing injection sites on the spore solution jars.


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Offlinedjsage420
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: djsage420]
    #4635528 - 09/08/05 10:34 PM (12 years, 14 days ago)

No you have to sterilize substrate spore will die if they run through a pressure cooker.


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Offlinedjsage420
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Re: A new spore syringe method hopefully correct me if i'm wrong [Re: djsage420]
    #4635542 - 09/08/05 10:36 PM (12 years, 14 days ago)

What I do is make a print inside sterile jar, inject 3-4 syringes of sterile water in, shake solution, I like very dark spore syringes because I just make liquid cultures with them, I use 1cc at a time for LC's and one Lc does about 60 jars for me.


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