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Offlinetokey666
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Slow colonization, whats the deal?
    #4631336 - 09/07/05 08:40 PM (12 years, 16 days ago)

Exactly 2 weeks, and 1 day ago, I inoculated 6 PF cake 1/2 pint jars. Well, only 4 have some signs of colonization.  And the 4 that do have signs of colonization, have about the a quarters size worth all in only one place. (I poked 4 holes an inoculated at 4 sites in each jar.)

They are kept in 80 - 85 degrees, complete darkness. The tinfoil is off and holes uncovered (which seemed to help the already colonizing ones.)There is no condensation in the bottom of the jars either.

The only think I can chalk this up to is weak sperm, lol. Could it be the spores weren't entirely up to par since I got them from FSR and there is no telling who made them? I left ample time for them spores to rehydrate as well. (In other words, I am doing everything right. :smile: )

I reinnoculated the other 2 today with the rest of the syringe that was left over. This will throw my timing off, but still.

So does anyone have any ideas as to why they 4 would colonize when the other two would not? That just puzzles me.

Thanks!!


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OfflineNilsen9491
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4631397 - 09/07/05 08:56 PM (12 years, 16 days ago)

You'll probably be bombarded with many answers to this question, and all will probably be valid to some degree. I am fairly new to the whole cultivation process, so I can, at times, relate pretty well with some questions. Tell me, did you let the jars dry COMPLETELY before inoculation? If not, you might have toasted your spores.

I've got 6 1 pint jars with golden teachers going right now. I inoculated a week and a half ago, and I'm about 80% covered as it stands now.

Do you have them in an incubation chamber? what did you use for substrate? Did you pack the jars tight or really loose?


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InvisibleOldSpice
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4631423 - 09/07/05 09:00 PM (12 years, 16 days ago)

To much water
To little water
Not shaking syringes
It could be a lot of things causing slow colonization


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Offlinetokey666
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Re: Slow colonization, whats the deal? [Re: Nilsen9491]
    #4631457 - 09/07/05 09:06 PM (12 years, 16 days ago)

Thanks for the reply.  I let the jars cool for 36 hours after sterilization in the PC. So I am pretty sure they arn't toasted. The water I i used in the syringes was also cooled for 24 hours.

They are in an incubation chamber. With a heating pad under a folded priority mail box. The temps stay perfect.  For substrate, I used verm, water and BRF (grinded myself in coffee grinder).  The jars were packed very loose, well...they were not packed actually. Just shaken horizontally to level out the top.

Another f-up variable could be my water. I have well water (which tastes just as good as water from a bottle.) I figured it would be fine. It works with my other grows. :laugh:  SO, i dont know if thats a factor or not.

Another variable could be the extra 30 minutes of sterilization time that I sterilized the jars in the PC. They were in there for 1 hr 15 minutes.

Thanks again!


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Offlinetokey666
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4631476 - 09/07/05 09:10 PM (12 years, 16 days ago)

Alounacara:
I used the exact amount of water required.
And I shook the syringes before innoc.


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OfflineShroomArtist84
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Re: Slow colonization, whats the deal? [Re: OldSpice]
    #4631479 - 09/07/05 09:10 PM (12 years, 16 days ago)

are there any signs of yellow or contam in the cakes?
maybe the area you have them in is too hot, causing this issue due to heat is normal!!


--------------------
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OfflinePsyWonderCap
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4631492 - 09/07/05 09:12 PM (12 years, 16 days ago)

i would say take one of your non colinized jars and pop it open and check the moisture. I would also think back..."Did i shake the syringe till my arm hurt and gave a lil shake inbetween jars...." If not there is something since you said you didnt have growth on all 4 spots. Anyways GL with the ones that have started


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InvisibleOldSpice
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4631514 - 09/07/05 09:15 PM (12 years, 16 days ago)

Quote:

tokey666 said:
Alounacara:
I used the exact amount of water required.
And I shook the syringes before innoc.



How long has it been after inoculation?
Are you guys watching that INXS thing on CBS? Its damn good :cool: :thumbup:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Offlinetokey666
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4631590 - 09/07/05 09:29 PM (12 years, 16 days ago)

One at a time! LOL.

No signs AT ALL of contams. No yellows, off colors, spider webby things, greens.  As I have said, the heat according to TWO thermometers, one digital and one mercury, sitting between the jars, is 80-85 degrees.

PsyWonderCap: You mean contaminzate the uncolonized jar to check moisture? I'd rather take my chances, lol. But as far as shaking the syringes, I did shake rather ..... whats the word..... overabundantly, but not till my arm hurt. :smile: And I did shake between each innoc.

Alounacara: Look at the first words in my first sentence in my first paragraph of the first post. :smile: I inoculated 2 weeks 1 day ago.  I am really not a fan of that show though. Well, I'm not really a fan of any prime time couch potato american reality tv specials. (Yes, im one of those people. LMFAO.)

Again, thanks for all the posts people!


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Invisibledemiu5
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4631757 - 09/07/05 10:03 PM (12 years, 16 days ago)

i've read before that the temp inside the jars is warmer than outside. If your heat is getting up to 85 outside...couldn't they be getting close to 90 or more inside? And isn't primo temp around 80-83? Just trying to put to use what i think i've learned along the way.


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InvisibleOldSpice
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4631778 - 09/07/05 10:10 PM (12 years, 16 days ago)

Yeah i know what your getting at but i grew up with INXS and good X
Its a really exceptional one
I pretty much hate most of whats pumped to us in the guise of good music on tv but this ones a rockkin one :smirk: :thumbup:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Offlinetokey666
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Re: Slow colonization, whats the deal? [Re: OldSpice]
    #4634586 - 09/08/05 06:10 PM (12 years, 15 days ago)

So none of these variables could have affected the slow colonization?

1. PCing for 1 hr 15 minutes.
2. Using tap water.
3. Weak spores from the FSR?

Mainly, I am looking for someone who has done these above methods and noticed slow colonization...


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OfflinePsychosMind
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4634601 - 09/08/05 06:13 PM (12 years, 15 days ago)

Weak spores can clearly effect alot of things, but pressure cooking I seriously doubt would affect anything, as long as you did it. I mean unless your cakes dried out then the amount of time you PC shouldn't really have much effect after the recommended amount of time...


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I'm one of those hugging crazies, as opposed to the stabbin' crazies


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OfflineDarkDisciple
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Re: Slow colonization, whats the deal? [Re: PsychosMind]
    #4634737 - 09/08/05 06:51 PM (12 years, 15 days ago)

What about stunted growth due to CO2 levels, try turning the jars upside down, the heavy CO2 will just fall out the bottom


--------------------
Pride is a Fool's Version of Wisdom
-Anonymous

"Don't study the idea to death with experts and committees. Get on with it and see if it works."

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OfflineBlackCat0315
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Re: Slow colonization, whats the deal? [Re: DarkDisciple]
    #4634863 - 09/08/05 07:33 PM (12 years, 15 days ago)

Hey there,
I had the same problem with one of my recent jars...7 out of 8 started showing decent growth by day 7, but 1 showed nothing...I started to toss it but waited and extra week and it started to show some positive signs...myc growth at the inoculation points.

So my advice is wait another week and if no growth then - toss it.

Good Luck !


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Offlinetokey666
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Re: Slow colonization, whats the deal? [Re: BlackCat0315]
    #4634902 - 09/08/05 07:44 PM (12 years, 15 days ago)

Intersting stuff. I will do just that.

DarkDisciple: I thought that co2 was only made after the myc started to grow and emitted their waste. I have no growth, so would flipping really make a difference at this point?

BlackCat: You had decent growth at day 7 you said? Wow. It's been day 16 and I am just now getting decent growth. Live and learn!

Thanks all!


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Offlinelilswift
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4635082 - 09/08/05 08:35 PM (12 years, 15 days ago)

dam, that sucks, my jars grow fast.


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Offlinetokey666
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Re: Slow colonization, whats the deal? [Re: lilswift]
    #4635134 - 09/08/05 08:58 PM (12 years, 15 days ago)

Thanks for that. :frown:

Care to share you secrets since you do so well?


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Offlineredood
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Re: Slow colonization, whats the deal? [Re: tokey666]
    #4635199 - 09/08/05 09:14 PM (12 years, 15 days ago)

I would have to agree with demius it sounds like your problem might lie in your temps if you can back it down about 4 degrees 82 is plenty and mine showed signs of colonization within 4 days at those temps also if you are measuring temps from the tops of the jars since your heat is underneath it is more than likely your temps are higher than what you think.


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OfflineWhiteBunny
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Re: Slow colonization, whats the deal? [Re: redood]
    #4635427 - 09/08/05 10:07 PM (12 years, 15 days ago)

how many days is normal for the start of grow?


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