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OfflineB.I.O.
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2nd flush of samuiensis (pics)
    #461135 - 11/16/01 12:55 AM (22 years, 5 months ago)

this is the second flush...one tray had to be discarded cause of mold...also they reached full maturity of their gills (2nd pic)




mj.....do you want a print of those for closer identification?


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OfflineB.I.O.
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #461136 - 11/16/01 01:00 AM (22 years, 5 months ago)

also notice how blackblue the stem turned where i cut it (lower right)...i got shitloads of unpeeled oat-grain....so im gonna try that next as a substrate...

BiO.

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Invisiblefuzzysquirelnuts
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Registered: 06/22/01
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #461235 - 11/16/01 06:43 AM (22 years, 5 months ago)

they look like theyre a small type mushroom
are they potent?


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OfflineDinoMyc
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Registered: 11/13/99
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #461239 - 11/16/01 06:54 AM (22 years, 5 months ago)

:) beauty


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If I made affront, I apologize.
If I made affirmation, I apologize.
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OfflineB.I.O.
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Re: 2nd flush of samuiensis (pics) [Re: DinoMyc]
    #462110 - 11/18/01 09:53 AM (22 years, 5 months ago)

id say that they are pretty potent what my dog reported....similar to tampa or mex what he said....

BiO.

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Invisibleralphster44
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #462286 - 11/18/01 12:44 PM (22 years, 5 months ago)

Post deleted by administrator.


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OfflineDarK_SavioR
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #462610 - 11/18/01 06:19 PM (22 years, 5 months ago)

Nice, those mushrooms look pretty cool cause of how short they are, heh. How do you go about growing the species? (what does it colonize, any special casings, temp and humidity?)


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InvisibleKOPELANDIAA
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #465735 - 11/21/01 03:32 PM (22 years, 5 months ago)

wow, they look so cuuute ...
is it possible to know more about this beautiful and sympathic strain ? Thanks in advance ...

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OfflineB.I.O.
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Re: 2nd flush of samuiensis (pics) [Re: KOPELANDIAA]
    #466419 - 11/22/01 05:28 AM (22 years, 5 months ago)

this cute species was detected first by john allen aka mjshroomer in 1990 on koh samui island off thailand...1994 i guess this species was fruited by jochen gartz at university of leipzig....cultures or spores were not meant to circulate exept with permission of j. allen....
i got the culture from a german shroomcompany and j. allen was pretty pissed that a company sold "his" shroom without permission...
this shroom resembles very much the tampa&mexi...just smaller and fatter (well i think this is a result of the casing)
if MJ gives me the permission i will trade a few prints to some fellowgrowers....this beauty should be grown!!!! (not only by me)

BiO.

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InvisibleKOPELANDIAA
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Re: 2nd flush of samuiensis (pics) *DELETED* [Re: B.I.O.]
    #466434 - 11/22/01 06:08 AM (22 years, 5 months ago)

Post deleted by KOPELANDIAA

Reason for deletion: paranoia


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Offlinenuminousphish
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #466457 - 11/22/01 06:45 AM (22 years, 5 months ago)

No spore trade/sales allowed? What kind of crap is that? I like mjshroomer and I feel his presence is a boon to this forum and mycology in general, but to get angry because someone cultivates a strain he found is insane. Did he genetically engineer this petite psilocybe, then patent it? What possible reason could he have for not wanting people to enjoy such a beautiful specimin?

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OfflinestonErollEr1
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Re: 2nd flush of samuiensis (pics) [Re: numinousphish]
    #466471 - 11/22/01 07:06 AM (22 years, 5 months ago)

That wasent the reason..It was bqause the one that sold it must have gotten it illigal in some way..there is a thread about that perticular topic...search the vendor forum..
Mj wasnt angry cuz of the cultivation...but the way they obtained the speices...I think he does sell some prints or was about to..
peace
stonErollEr

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Invisiblemjshroomer
Sage
Registered: 07/21/99
Posts: 13,774
Loc: gone with my shrooms
Re: 2nd flush of samuiensis (pics) [Re: stonErollEr1]
    #466540 - 11/22/01 09:45 AM (22 years, 5 months ago)

There is something very funny about those mushrooms which you arre growing as P. samuiensis.

One is that the stems look to thick for P. samuiensis and also that the caps become wavy like P. cyanescens.

P. samuiensis stems are an off whitish to straw yellowish color and the caps all have impressive nipples and umbos on many.

Your caps become very wavy which is simialr to other species. The P. mexicana caps curl up on the left and right and all of yours become wavy.

Here is a picture of P. samuiensis.



Yes B.I.O., I wouldl like a print to have their identity confirmed.

ANd No I am ticked off when people in europe are selling alleged cultures of this strain since their were no spore prints available from me.

Gartz did grow several groups of this shroom but the first cultivation took longer than 6 months to get four shrooms to fruit.

I have written Gartz about this but have not received a response.

Gartz has no email since he got in trouble in Holland for selling cultures of cubies to people in Amsterdam. He does not tewll me any thing about his legal hasle witht he Nederlands goverment. But he refuses to have an email address.

The mushrooms in your pictures look awful thick to be P. samuiensis.

I will post another image of some in the field and some picked here to compare them with what you have shown.

You can send a print to me for verification of species at:

Exotic Forays,
P.O. Box 45164,
Seattle, Washington,
98105,
U.S.A.

Mj

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Invisiblemjshroomer
Sage
Registered: 07/21/99
Posts: 13,774
Loc: gone with my shrooms
Re: 2nd flush of samuiensis (pics) [Re: mjshroomer]
    #466542 - 11/22/01 09:59 AM (22 years, 5 months ago)

Here are two images. No shrooms are larger than 3 inches high.

Here is a fresh growing image of a small colony of P. samuiensis, which by the way have just been collected inthe NE of Thailand.

And here is a picture of picked specimens a four by 6 inch photo and the paper on the page (white) is 11 inches long.

.

mj



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Invisiblemjshroomer
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Re: 2nd flush of samuiensis (pics) [Re: stonErollEr1]
    #466553 - 11/22/01 10:38 AM (22 years, 5 months ago)

Well after seeing the pictures posted by B.I.O, I wonder why such a difference in cultivation of this species with p. samuiensis.

Here is Gartz's method of cultivation for the specimens he grew in his lab at the University of L:eipzig, Germany.

I am also posting below, his cultivation photograph of this species.



Hm, could not find the color image of this one. Must be on a slide.

So here is the black and white version

mj


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Invisiblemjshroomer
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Posts: 13,774
Loc: gone with my shrooms
Re: 2nd flush of samuiensis (pics) [Re: mjshroomer]
    #466556 - 11/22/01 10:46 AM (22 years, 5 months ago)

Not sure if I posted this or not.

Here is Gartz's cultivation of this species. From Gartz, Allen and Merlin, 1993. Journal of Ethnopharmacology.

Mycelium was obtained from the spores of a dried specimen of P. samuiensis by methods described by Stamets and Chilton (1983) and was stored as stock culture on 6% malt agar. Strains on agar of a related species Psilocybe tampanensis Guzm?n and Pollock and P. semilanceata from Germany were also obtained. In a ratio of 1 to 6% on malt agar, the whitish mycelium of P. samuiensis grew at a faster pace than that of similar mycelium of P. semilanceata. The rapid growth of P. tampanensis was similar to the growth of P. samuiensis; however, the former species soon formed brownish sclerotia on the agar as it does with P. mexicana (Stamets and Chilton 1983). Even after a relatively long growth period (3 months), the mycelium of P. samuiensis formed only a few small brownish sclerotia.

Similar conditions were observed while cultivating the three species on Lolium seed / water (1:1.5). The same conditions were also observed in complete darkness; and P. tampanensis and P. samuiensis both grew with rapid speed. Observations on the rapid formations of sclerotia in P. tampanensis after a few weeks of cultivation has already been reported by Stamets and Chilton (1983). In contrast, P. samuiensis under cultivation only formed thick whitish mycelium (rhizomorphs, diameter 2 to 3 millimeters) throughout the media, and produced no sclerotia. Under the same conditions of cultivation, P. semilanceata grew slowly, producing only a fine and whitish mycelium with no formation of sclerotia or rhizomorphs.

Psilocybin was found to be present in the cultured, non-bluing mycelium of P. samuiensis grown on 6% malt agar. Amounts of psilocybin, ranging from 0.24 to 0.32% dry weight, were analyzed in 5 different batches of mycelium grown over a four week period. Analyses also revealed that these quantities of psilocybin were much lower than those detected in the naturally occurring fruit bodies obtained from the field. Interestingly, no other indole derivatives were detected in the extracts of the in vitro grown mycelium.

The alkaloidal levels obtained from the slightly bluing sclerotia of P. tampanensis were high. Additionally, the amount of psilocybin obtained from five different cultivations grown on 6% malt agar and Lolium seed ranged from 0.34 to 0.68% by dry weight, and from 0.41 to 0.61% in three sections of sclerotia obtained from a single cultivation on Lolium seed. The sclerotia obtained from malt agar also contained 0.21 to 0.52% psilocin, but no baeocystin was detected. The sclerotia obtained from Lolium had a concentration of psilocin from 0.11 to 0.32%. Until now, it was not possible to produce complete fruit bodies of P. samuiensis on either malt agar or Lolium seed. Some small incomplete fruit bodies of P. samuiensis (up to 2 centimeters high) appeared, but failed to develop into normal sporulating mushrooms. These premature formations only occurred on agar with a low concentration of malt (0.5 to 1.5%). After stopping their natural growth, these incomplete fruit bodies began to exhibit a slight spontaneous bluing reaction.

At this time it was not possible to be able to cultivate mushrooms on a Lolium seed / water mixture (Stamets and Chilton. 1983). Psilocybe samuiensis also grows well on some grains such as rye or rice. A mixture of rye/horse dung/water (2:1:2) did produce fruit bodies of P. samuiensis after 4 months cultivation, and 3 weeks after casing with peat/chalk (2:1) (Stamets and Chilton, 1983) (fig. 2). Two flushings producing eight mushrooms were observed; six of the mushrooms were analyzed (see fig. 2 and table 1).

Mj

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OfflineB.I.O.
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Re: 2nd flush of samuiensis (pics) [Re: mjshroomer]
    #466599 - 11/22/01 12:15 PM (22 years, 5 months ago)

sounds interesteng....welll....whatever im groing is a very potent shroom....from all selections no sclerotia were detected until now....

got your addy mj....i will send you a print the next days

BiO.

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Invisiblemjshroomer
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Registered: 07/21/99
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #466764 - 11/22/01 04:24 PM (22 years, 5 months ago)

Thank you B.I.O,

mj

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Offlineazurescen
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Re: 2nd flush of samuiensis (pics) [Re: KOPELANDIAA]
    #469151 - 11/25/01 11:10 AM (22 years, 4 months ago)

If you get permission, please pm me. I would love to do some work with this species


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if the sign says dont pick the mushrooms, you should probably pick them

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OfflineB.I.O.
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Re: 2nd flush of samuiensis (pics) [Re: azurescen]
    #474349 - 11/30/01 02:04 AM (22 years, 4 months ago)

i hope MJ got my letter already ...so if he checks the spores we will know more....if he gives me permission i will trade a few prints.

BiO.

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InvisibleLuciferX
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #475151 - 11/30/01 05:59 PM (22 years, 4 months ago)

what were your colonizing and fruiting temps? Id love to try this species out.


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OfflineB.I.O.
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Re: 2nd flush of samuiensis (pics) [Re: LuciferX]
    #475341 - 11/30/01 08:55 PM (22 years, 4 months ago)

colonizing temps were around 78?F and fruiting around 70ties...spawn was made from perennial rye-grasseed in filterbags...crumbled...recovered2days...cased with peat/lime

BiO.

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Offlinestefan
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Re: 2nd flush of samuiensis (pics) [Re: B.I.O.]
    #475704 - 12/01/01 03:00 AM (22 years, 4 months ago)

very nice

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