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Shop: Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate, Injection Grain Bag   JK Botanik Super Blended Yellow Maeng Da

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Registered: 11/08/01
Posts: 28
Last seen: 21 years, 6 months
innoculation and sterilization
    #460262 - 11/16/01 08:45 AM (21 years, 10 months ago)

I really don't understand why such serious precautions are taken during the innoculation phase like wearing a mask and lysoling the place, but after that it is not so serious like the jars are exposed to air and contaminants if it has holes drilled in. Why is t so important that during those few seconds that everything is so sterile but after that the environment doesn't have to be so clean? Thanks

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Registered: 10/28/01
Posts: 107
Last seen: 18 years, 1 month
Re: innoculation and sterilization [Re: kgb187]
    #460272 - 11/16/01 08:53 AM (21 years, 10 months ago)

Once you inoculate you cover the holes with tape or foil.
So no air goes in it.
While you incoulate, instead, air may come in thru the holes and lots of bacteria may attach to the syringe which goes inside the substrate.

As evil as a dwarf can get...

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