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OfflineDingus
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Registered: 08/28/05
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Cloning with PF tek
    #4591259 - 08/28/05 04:48 PM (12 years, 7 months ago)

I'm new to this, so please bear with me.

I like the PF tek because it looks like I can use a single substrate/jar to go from spores all the way to fruiting. However, I'm only somewhat interested in growing anything form spores; my interest is more academic, to see if I can successfully grow a mushroom.

That said, growth from spores offers no advantage for me, compared to grabbing a mushroom from the back yard and cloning it. The question is, I'd rather not have to culture on cardboard, for example, and then transfer to a jar later. Could I use tissue samples instead of spore with the PF tek?

Thanks in advance.


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Offlinedjsage420
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Re: Cloning with PF tek [Re: Dingus]
    #4591281 - 08/28/05 04:59 PM (12 years, 7 months ago)

It would be hard to keep everything sterile for that
Make a liquid culture with it Karo syrup and water


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OfflineShaw
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Re: Cloning with PF tek [Re: Dingus]
    #4591282 - 08/28/05 04:59 PM (12 years, 7 months ago)

I would imagine it could work. You'd have to remove the lid completely to put the tissue sample in. Since your completely academic, I'd try to get some fresh button mushrooms from the grocery store. I've seen them grow mycelia in the fridge before i got a chance to eat them. there is no gaurentee what you pick out of the yard can even be cultivated. Also i guess only fill your jar half full with substrate so you can view for contaminates and do several to be sure you get a good one.


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OfflineDingus
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Re: Cloning with PF tek [Re: djsage420]
    #4591287 - 08/28/05 05:00 PM (12 years, 7 months ago)

And then inoculate the PF jar with the liquid culture?

Considering that I can't serilize it once the culter has been started, how does that actually offer a sterilization advantage? Also, should not the interior of the mushroom be sterile?

Thanks


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Offlinedjsage420
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Re: Cloning with PF tek [Re: djsage420]
    #4591317 - 08/28/05 05:12 PM (12 years, 7 months ago)

It would be hard to shove tissue into a pf cake without contams, If you plop the tissue into a LC you would only need lid open for a breif second, to me that is a great reduction in contam risk.
And instead of your clone innoculating one jar you can make hundreds with the LC


http://www.shroomery.org/index/par/8005 = LC tek


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If you put your head to the grass, you can hear it growing


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OfflineDingus
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Re: Cloning with PF tek [Re: Dingus]
    #4591332 - 08/28/05 05:17 PM (12 years, 7 months ago)

I get it now, good point.

The LC tek you posted works fine with a piece of tissue instead of spores then?


Edited by Dingus (08/28/05 05:19 PM)


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Offlinedjsage420
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Re: Cloning with PF tek [Re: Dingus]
    #4591349 - 08/28/05 05:23 PM (12 years, 7 months ago)

Yep tissue will work fine you can substitute honey for karo syrup its a bit cheaper and stays clear so its easier to see contams.


--------------------
If you put your head to the grass, you can hear it growing


Edited by djsage420 (08/28/05 05:24 PM)


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