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OfflineMrKrinkle
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Registered: 08/23/05
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Last seen: 3 years, 5 months
Flame Sterilizing
    #4588028 - 08/27/05 06:45 PM (11 years, 8 months ago)

I got a syringe last night filled with spores, and im wondering should i still flame sterilize it?

i probobly will just to be on the safe side, but in order to flame sterilize a needle, it gets really hot, and wouldn't that just melt the needle right out of the plastic part?

or am i just thinking weird?


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InvisibleHolydiver
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Re: Flame Sterilizing [Re: MrKrinkle]
    #4588031 - 08/27/05 06:47 PM (11 years, 8 months ago)

You can flame sterilize if you want, but it's definitely not necessary. Swab w/ alcohol and quick hands is good enough.


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To find a place to live between the negatives and positives.


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OfflinetheBRINK
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Re: Flame Sterilizing [Re: Holydiver]
    #4588035 - 08/27/05 06:48 PM (11 years, 8 months ago)

if you do flame sterilize it... jus dun get the flame too close to the plastic and you'll be ok... alcohol works fine too


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Invisiblesrgtm1a
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Re: Flame Sterilizing [Re: theBRINK]
    #4588074 - 08/27/05 07:00 PM (11 years, 8 months ago)

just flame the tip, not the whole needle. Stay away from the plastic. Alcohol should be fine though....but I always play it safe by:

swabbing with alcohol, flamming the needle, then swabbing again, then inject. It is overkill but that's the way I do it.


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InvisiblePrisoner#1M
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Re: Flame Sterilizing [Re: srgtm1a]
    #4588082 - 08/27/05 07:02 PM (11 years, 8 months ago)

overkill prevents contams, I do the same as you only I flame the whole needle


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Invisiblemycogirl
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Re: Flame Sterilizing [Re: srgtm1a]
    #4588158 - 08/27/05 07:33 PM (11 years, 8 months ago)

Quote:

srgtm1a said:
just flame the tip, not the whole needle. Stay away from the plastic. Alcohol should be fine though....but I always play it safe by:

swabbing with alcohol, flamming the needle, then swabbing again, then inject. It is overkill but that's the way I do it.




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Edited by mycogirl (03/14/06 03:01 AM)


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Invisiblesrgtm1a
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Re: Flame Sterilizing [Re: mycogirl]
    #4588194 - 08/27/05 07:48 PM (11 years, 8 months ago)

well, I think that is the most important part of the whole growth process.  I am the most careful with injecting.

After that, I'm not so nutz on sterility.  I'm not one of those people that sprays the room down with lysol, or use h202 to dunk or heppa filters, or any of that shit.  So long as you work in a clean environment, you don't have to be a sterility nazi.

my best friend is rubbing alcohol :grin:


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InvisibleOldSpice
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Re: Flame Sterilizing [Re: Prisoner#1]
    #4588249 - 08/27/05 08:07 PM (11 years, 8 months ago)

Quote:

Prisoner#1 said:
overkill prevents contams, I do the same as you only I flame the whole needle



Huh really? i just use 91% alcahol and the only time it contammed was when i was doing some stupid shortcut
I have a shitload of Qt jars w/corn and none of them have contammed


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Paw Paw be thy Name....
My friends are thirsty


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InvisiblePrisoner#1M
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Re: Flame Sterilizing [Re: OldSpice]
    #4588294 - 08/27/05 08:23 PM (11 years, 8 months ago)

flame it, wipe it and you;ll still not have contams... you trying to take another short cut buddy  :smirk:


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InvisibleOldSpice
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Re: Flame Sterilizing [Re: Prisoner#1]
    #4588307 - 08/27/05 08:28 PM (11 years, 8 months ago)

Ive been just using alcahol for probably 3+ yrs now
I only got contams bad when i dirty printed that EQ strain you isolated, That was a damn good strain :smile: :smile:
Let me in ... :smirk:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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InvisiblePrisoner#1M
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Re: Flame Sterilizing [Re: OldSpice]
    #4588316 - 08/27/05 08:31 PM (11 years, 8 months ago)

Quote:

Alounacara said:
Ive been just using alcahol for probably 3+ yrs now




like I said, I'm ultra paranoid but I also experiment with contams so I need to be



Quote:

Let me in ... :smirk:




I be workin on it


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InvisibleOldSpice
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Registered: 08/25/03
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Re: Flame Sterilizing [Re: Prisoner#1]
    #4588332 - 08/27/05 08:35 PM (11 years, 8 months ago)

You know ill behave :smirk:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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InvisiblePrisoner#1M
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Re: Flame Sterilizing [Re: OldSpice]
    #4588391 - 08/27/05 08:46 PM (11 years, 8 months ago)

I know you will, unfortunately it's not my call.


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InvisibleOldSpice
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Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Flame Sterilizing [Re: Prisoner#1]
    #4588440 - 08/27/05 08:57 PM (11 years, 8 months ago)

Yep its kinda shitty


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
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