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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Rye Grain, Vermiculite

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OfflinePhake_ld
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Registered: 10/08/01
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COLINIZATION QUESTION
    #458619 - 11/14/01 11:06 PM (15 years, 30 days ago)

it's been a week and I still dont have any sign of colonization... I innoculated 1 pint jars with 2cc of Purto's, and some with Treasure Coast. I've placed them in my incubator which temperature is kept between 80F-86F.

There are no white spots. There arent any noticable spots. The only thing i've noticed is that my subsrate is starting to seperate at certain areas within the jar. Is this a sign of colonization?

Maby im being impatient, but what can i expect?


Edited by Phake_ld (11/14/01 11:42 PM)


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Invisibleblahblahblah
Mad Scientist

Registered: 10/25/01
Posts: 1,022
Loc: South America
Re: COLINIZATION QUESTION [Re: Phake_ld]
    #458789 - 11/15/01 01:27 AM (15 years, 30 days ago)

What are you useing for substrate? Usually the reason my jars don't grow is because I mixed the substrate too dry, actually I have a few jars that are this way and have been this way for a while. Pretty sure its because they dont have enough moisture.


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: blahblahblah]
    #458805 - 11/15/01 01:34 AM (15 years, 30 days ago)

Im using brown rice flower with Verm. I followed MMGG tek to the T... So I should have noticed some sign of colonization by now?

If so, is there anything I can do to correct this problem?


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OfflineSnobrdr311
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Re: COLINIZATION QUESTION [Re: Phake_ld]
    #458829 - 11/15/01 01:46 AM (15 years, 30 days ago)

give it a few more days dude, I use Dark Rye Flour and verm instead of BRF, it seems to colonise a LOT faster!

give it another week, if nothing happens then start over. maybe you didn't wait long enough after boiling/pressure cooking


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: Snobrdr311]
    #458857 - 11/15/01 02:13 AM (15 years, 30 days ago)

Dark Rye Flower is mixed the same way as brown rice flower. I pressure cook for an hour in a commercial pressure cooker. Is this long enough?


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OfflineSnobrdr311
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Re: COLINIZATION QUESTION [Re: Phake_ld]
    #458866 - 11/15/01 02:25 AM (15 years, 29 days ago)

yup. mixed the exact same way, 1/4,1/4/2/3 ...boil for an hour, don't know about pressure cooking, I think it's less than an hour though, 15 PSI at like 30-45 mins I believe? check the teks on this website


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Offlinemastreo
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Registered: 11/06/01
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Re: COLINIZATION QUESTION [Re: Snobrdr311]
    #458928 - 11/15/01 04:07 AM (15 years, 29 days ago)

What's the difference between dark rye flour and rye flour?


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Offlinemycocitizen
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Registered: 10/30/01
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Re: COLINIZATION QUESTION [Re: mastreo]
    #458952 - 11/15/01 04:34 AM (15 years, 29 days ago)

did you let the jars thouroughly cool down before inoculating. I know the first time that I inoculated jars i was so impatient to get it done that i sprayed the spores in when the jars were still warm to the touch, and I did not see any colonization for about 2 1/2 to 3 weeks. But after that tehy took off and I was happily successful with my first batch.


--------------------
"Well why not? Anything worth doings, worth doing right"


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: mycocitizen]
    #458959 - 11/15/01 04:38 AM (15 years, 29 days ago)

yah, they wer room temperature, no warmth coming out of them what so ever!

Is there a way to inject more moisture into the jars of dry rice cakes without introducing contaminents (Assuming the jars became dry)?


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Offlinemycocitizen
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Re: COLINIZATION QUESTION [Re: Phake_ld]
    #458961 - 11/15/01 04:41 AM (15 years, 29 days ago)

I have read that you can inject more moisture using syringes that are sterile. Make sure you sterilize a syringe or syringes very well, boil some water and suck it up into a syringe and wait for it to cool. Once it has cooled put it in the same holes you used to inoculate and spray in about a 1/2 to 1cc of water at each site.


--------------------
"Well why not? Anything worth doings, worth doing right"


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: mycocitizen]
    #458965 - 11/15/01 04:47 AM (15 years, 29 days ago)

Roger, Roger!

I have read that you can inject more moisture using syringes that are sterile. Make sure you sterilize a syringe or syringes very well, boil some water and suck it up into a syringe and wait for it to cool. Once it has cooled put it in the same holes you used to inoculate and spray in about a 1/2 to 1cc of water at each site.


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Invisibledk138
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Registered: 04/18/01
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Loc: SOC
Re: COLINIZATION QUESTION *DELETED* [Re: Phake_ld]
    #459266 - 11/15/01 01:54 PM (15 years, 29 days ago)

Post deleted by dk138


--------------------
"And the worms ate into his brains."


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: dk138]
    #459270 - 11/15/01 01:58 PM (15 years, 29 days ago)

I let my first batch cool for about 24 hours, I let my second batch cool for about 16 hours. ANd Yes, I did get my spores from spore works!


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: dk138]
    #459273 - 11/15/01 02:03 PM (15 years, 29 days ago)

Should I have not gotten my spores from spore works?


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Invisibledk138
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Re: COLINIZATION QUESTION *DELETED* [Re: Phake_ld]
    #459283 - 11/15/01 02:15 PM (15 years, 29 days ago)

Post deleted by dk138


--------------------
"And the worms ate into his brains."


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: dk138]
    #459292 - 11/15/01 02:27 PM (15 years, 29 days ago)

Thanks, I will deffinately email him... Why did he send out low germination spore syringes anyways?


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: Phake_ld]
    #459295 - 11/15/01 02:31 PM (15 years, 29 days ago)

.... and how would he rectify this problem?


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OfflinePhake_ld
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Re: COLINIZATION QUESTION [Re: dk138]
    #459297 - 11/15/01 02:33 PM (15 years, 29 days ago)

really, How would he rectify this problem?


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Invisibledk138
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Loc: SOC
Re: COLINIZATION QUESTION *DELETED* [Re: Phake_ld]
    #459298 - 11/15/01 02:34 PM (15 years, 29 days ago)

Post deleted by dk138


--------------------
"And the worms ate into his brains."


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OfflineGoodTimes
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Registered: 08/13/01
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Last seen: 14 years, 10 months
Re: COLINIZATION QUESTION [Re: Phake_ld]
    #461072 - 11/17/01 03:20 AM (15 years, 27 days ago)

To speed up colinization it was said before I though it to increase room temp of friendly spore sprouts. They like it hot and MOIST. Nomally they should sprout and fluff 7 days after prime conditions. And colonize to the extreme they will. What you tink? Spark em up and spread dem with HIENZ. Cause de best tings come to does who wait. And even better tings come to those who waited already and know how not to wait?!


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Rye Grain, Vermiculite

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