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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
COLINIZATION QUESTION
    #458619 - 11/14/01 09:06 PM (22 years, 3 months ago)

it's been a week and I still dont have any sign of colonization... I innoculated 1 pint jars with 2cc of Purto's, and some with Treasure Coast. I've placed them in my incubator which temperature is kept between 80F-86F.

There are no white spots. There arent any noticable spots. The only thing i've noticed is that my subsrate is starting to seperate at certain areas within the jar. Is this a sign of colonization?

Maby im being impatient, but what can i expect?

Edited by Phake_ld (11/14/01 09:42 PM)

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Invisibleblahblahblah
Mad Scientist

Registered: 10/24/01
Posts: 1,022
Loc: South America
Re: COLINIZATION QUESTION [Re: Phake_ld]
    #458789 - 11/14/01 11:27 PM (22 years, 3 months ago)

What are you useing for substrate? Usually the reason my jars don't grow is because I mixed the substrate too dry, actually I have a few jars that are this way and have been this way for a while. Pretty sure its because they dont have enough moisture.

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: blahblahblah]
    #458805 - 11/14/01 11:34 PM (22 years, 3 months ago)

Im using brown rice flower with Verm. I followed MMGG tek to the T... So I should have noticed some sign of colonization by now?

If so, is there anything I can do to correct this problem?

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OfflineSnobrdr311
outdoorenthusiast
 User Gallery

Registered: 09/03/01
Posts: 1,468
Loc: Midwest
Last seen: 10 years, 4 months
Re: COLINIZATION QUESTION [Re: Phake_ld]
    #458829 - 11/14/01 11:46 PM (22 years, 3 months ago)

give it a few more days dude, I use Dark Rye Flour and verm instead of BRF, it seems to colonise a LOT faster!

give it another week, if nothing happens then start over. maybe you didn't wait long enough after boiling/pressure cooking

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: Snobrdr311]
    #458857 - 11/15/01 12:13 AM (22 years, 3 months ago)

Dark Rye Flower is mixed the same way as brown rice flower. I pressure cook for an hour in a commercial pressure cooker. Is this long enough?

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OfflineSnobrdr311
outdoorenthusiast
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Registered: 09/03/01
Posts: 1,468
Loc: Midwest
Last seen: 10 years, 4 months
Re: COLINIZATION QUESTION [Re: Phake_ld]
    #458866 - 11/15/01 12:25 AM (22 years, 3 months ago)

yup. mixed the exact same way, 1/4,1/4/2/3 ...boil for an hour, don't know about pressure cooking, I think it's less than an hour though, 15 PSI at like 30-45 mins I believe? check the teks on this website

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Offlinemastreo
journeyman
Registered: 11/06/01
Posts: 56
Last seen: 21 years, 9 months
Re: COLINIZATION QUESTION [Re: Snobrdr311]
    #458928 - 11/15/01 02:07 AM (22 years, 3 months ago)

What's the difference between dark rye flour and rye flour?

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Offlinemycocitizen
journeyman
Registered: 10/30/01
Posts: 55
Loc: USA
Last seen: 21 years, 11 months
Re: COLINIZATION QUESTION [Re: mastreo]
    #458952 - 11/15/01 02:34 AM (22 years, 3 months ago)

did you let the jars thouroughly cool down before inoculating. I know the first time that I inoculated jars i was so impatient to get it done that i sprayed the spores in when the jars were still warm to the touch, and I did not see any colonization for about 2 1/2 to 3 weeks. But after that tehy took off and I was happily successful with my first batch.


--------------------
"Well why not? Anything worth doings, worth doing right"

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: mycocitizen]
    #458959 - 11/15/01 02:38 AM (22 years, 3 months ago)

yah, they wer room temperature, no warmth coming out of them what so ever!

Is there a way to inject more moisture into the jars of dry rice cakes without introducing contaminents (Assuming the jars became dry)?

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Offlinemycocitizen
journeyman
Registered: 10/30/01
Posts: 55
Loc: USA
Last seen: 21 years, 11 months
Re: COLINIZATION QUESTION [Re: Phake_ld]
    #458961 - 11/15/01 02:41 AM (22 years, 3 months ago)

I have read that you can inject more moisture using syringes that are sterile. Make sure you sterilize a syringe or syringes very well, boil some water and suck it up into a syringe and wait for it to cool. Once it has cooled put it in the same holes you used to inoculate and spray in about a 1/2 to 1cc of water at each site.


--------------------
"Well why not? Anything worth doings, worth doing right"

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: mycocitizen]
    #458965 - 11/15/01 02:47 AM (22 years, 3 months ago)

Roger, Roger!

I have read that you can inject more moisture using syringes that are sterile. Make sure you sterilize a syringe or syringes very well, boil some water and suck it up into a syringe and wait for it to cool. Once it has cooled put it in the same holes you used to inoculate and spray in about a 1/2 to 1cc of water at each site.

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Invisibledk138
enthusiast
Registered: 04/18/01
Posts: 185
Loc: SOC
Re: COLINIZATION QUESTION *DELETED* [Re: Phake_ld]
    #459266 - 11/15/01 11:54 AM (22 years, 3 months ago)

Post deleted by dk138


--------------------
"And the worms ate into his brains."

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: dk138]
    #459270 - 11/15/01 11:58 AM (22 years, 3 months ago)

I let my first batch cool for about 24 hours, I let my second batch cool for about 16 hours. ANd Yes, I did get my spores from spore works!

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: dk138]
    #459273 - 11/15/01 12:03 PM (22 years, 3 months ago)

Should I have not gotten my spores from spore works?

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Invisibledk138
enthusiast
Registered: 04/18/01
Posts: 185
Loc: SOC
Re: COLINIZATION QUESTION *DELETED* [Re: Phake_ld]
    #459283 - 11/15/01 12:15 PM (22 years, 3 months ago)

Post deleted by dk138


--------------------
"And the worms ate into his brains."

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: dk138]
    #459292 - 11/15/01 12:27 PM (22 years, 3 months ago)

Thanks, I will deffinately email him... Why did he send out low germination spore syringes anyways?

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: Phake_ld]
    #459295 - 11/15/01 12:31 PM (22 years, 3 months ago)

.... and how would he rectify this problem?

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 21 years, 7 months
Re: COLINIZATION QUESTION [Re: dk138]
    #459297 - 11/15/01 12:33 PM (22 years, 3 months ago)

really, How would he rectify this problem?

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Invisibledk138
enthusiast
Registered: 04/18/01
Posts: 185
Loc: SOC
Re: COLINIZATION QUESTION *DELETED* [Re: Phake_ld]
    #459298 - 11/15/01 12:34 PM (22 years, 3 months ago)

Post deleted by dk138


--------------------
"And the worms ate into his brains."

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OfflineGoodTimes
newbie
Registered: 08/13/01
Posts: 34
Last seen: 22 years, 1 month
Re: COLINIZATION QUESTION [Re: Phake_ld]
    #461072 - 11/17/01 01:20 AM (22 years, 3 months ago)

To speed up colinization it was said before I though it to increase room temp of friendly spore sprouts. They like it hot and MOIST. Nomally they should sprout and fluff 7 days after prime conditions. And colonize to the extreme they will. What you tink? Spark em up and spread dem with HIENZ. Cause de best tings come to does who wait. And even better tings come to those who waited already and know how not to wait?!

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