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Offlinerachsrib
To fly you mustjump and forgetto hit theground

Registered: 08/08/05
Posts: 185
Last seen: 1 year, 1 month
An LC question
    #4584376 - 08/26/05 04:54 PM (11 years, 11 months ago)

Instead of preparing an Lc with liquid spore syringe could i just flake off a tiny bit of a spore print into the mixture. just looking to avoid an extra step but im not sure how sterile this would be? any help?


--------------------
"My father once said: If your afraid to die, make yourself hard to kill."


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 7 months
Trusted Cultivator
Re: An LC question [Re: rachsrib]
    #4584404 - 08/26/05 05:01 PM (11 years, 11 months ago)

Yes, as long as you are sterile about it. A glovebox, flood hood, or sterile room would be needed.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinerachsrib
To fly you mustjump and forgetto hit theground

Registered: 08/08/05
Posts: 185
Last seen: 1 year, 1 month
Re: An LC question [Re: scatmanrav]
    #4585478 - 08/26/05 10:59 PM (11 years, 11 months ago)

So the sterility of the print would not matter much?


--------------------
"My father once said: If your afraid to die, make yourself hard to kill."


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Offlinekingfish4200
shroom diddy

Registered: 08/12/05
Posts: 673
Last seen: 2 months, 22 days
Re: An LC question [Re: rachsrib]
    #4585494 - 08/26/05 11:04 PM (11 years, 11 months ago)

yes it does matter thats why he said to use a glovebox, flood hood, or sterile room. That will help keep things alot more sterile than just doing it in the open air where contam's are all around


--------------------
"The answer is never the answer. What's really interesting is the mystery. If you seek the mystery instead of the answer, you'll always be seeking. I've never seen anybody really find the answer-- they think they have, so they stop thinking. But the job is to seek mystery, evoke mystery, plant a garden in which strange plants grow and mysteries bloom. The need for mystery is greater than the need for an answer."

-- Ken Kesey


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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: An LC question [Re: rachsrib]
    #4585577 - 08/26/05 11:38 PM (11 years, 11 months ago)

Quote:

rachsrib said:
So the sterility of the print would not matter much?




yes, if the print is from a wild mushroom it's probably already contaminated, try
a little using a glove box and test it on a jar or try agar, if it's a print from
someone that didnt use clean room methods then it may have the same problem

most people are pretty good about keeping prints sterile, again, start with a small sample to test it, no point in loosing the whole print to a contam if it's
dirty, plus dirty prints give you an excuse later to experiment with agar


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