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Offlinehollisatp
Stranger
Registered: 06/01/05
Posts: 129
Loc: So Cal
Last seen: 16 years, 2 months
cambodian strains.. not growing
    #4571850 - 08/23/05 05:47 PM (19 years, 4 months ago)

second time trying a pf tek with a cambodian strain. first time all of the jars were contaminated. Now I cannot get them to grow at all. Is there a longer time for gestation than other spores? Temp is good and sterilization was great this time.. I think that last was because my cake were serilized a long time ago. Any help would be great..


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Don't let your meat loaf!

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Offlinepcubmycol
flattiefisherman

Registered: 05/23/05
Posts: 747
Loc: Selectively breeding the ...
Last seen: 16 years, 8 months
Re: cambodian strains.. not growing [Re: hollisatp]
    #4571895 - 08/23/05 05:59 PM (19 years, 4 months ago)

nope... you've got another problem. too wet maybe?


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Offlinehollisatp
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Registered: 06/01/05
Posts: 129
Loc: So Cal
Last seen: 16 years, 2 months
Re: cambodian strains.. not growing [Re: pcubmycol]
    #4571942 - 08/23/05 06:18 PM (19 years, 4 months ago)

any way to fix this?


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Don't let your meat loaf!

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OfflineMaJikFungus
There's a Fungusin my closet

Registered: 07/29/04
Posts: 252
Loc: School of Athens
Last seen: 15 years, 10 months
Re: cambodian strains.. not growing [Re: hollisatp]
    #4572093 - 08/23/05 06:58 PM (19 years, 4 months ago)

When did you inoculate these jars? Cambodian colonizes at a tremendous speed , one of the reasons itt's becoming my fav strain.

I inoculated some jars with a liquid culture I made from a cambodian print , and they were fully colonized in just over a week with the first signs of growth only two days from inoculation.


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Offlinepcubmycol
flattiefisherman

Registered: 05/23/05
Posts: 747
Loc: Selectively breeding the ...
Last seen: 16 years, 8 months
Re: cambodian strains.. not growing [Re: MaJikFungus]
    #4572127 - 08/23/05 07:05 PM (19 years, 4 months ago)

if it is too wet... there is a fix. Redo everything

but how long has it been since inoculation?

Happy Trails and GL to ya


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weretheshit.com is the shit!

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Offlinehollisatp
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Registered: 06/01/05
Posts: 129
Loc: So Cal
Last seen: 16 years, 2 months
Re: cambodian strains.. not growing [Re: pcubmycol]
    #4573555 - 08/24/05 12:10 AM (19 years, 4 months ago)

I inoculated them about 7 days ago..


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OfflineSilly_Cyben
not-quite-newbie

Registered: 04/11/05
Posts: 183
Loc: USA
Last seen: 1 year, 8 months
Re: cambodian strains.. not growing [Re: hollisatp]
    #4573669 - 08/24/05 01:03 AM (19 years, 4 months ago)

Give them another week. If your jars were sterilized long ago and if they weren't completely sealed, they may have lost too much moisture. Also, if you didn't shake the spore syringe between inoculations, there may have been few viable spores in what you injected. My Cambodians also colonized very quickly, showing growth in 3 days, but they produced only a few very large specimens, so I wasn't that happy with the strain overall. Others have had different results, I'm sure. Another reason for no growth can be if you inoculated the jars before they were completely cool. Cooling overnight is best, because even jars that feel cool to the touch may still be hot enough in the core to kill spores.

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Offlinehollisatp
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Registered: 06/01/05
Posts: 129
Loc: So Cal
Last seen: 16 years, 2 months
Re: cambodian strains.. not growing [Re: Silly_Cyben]
    #4573737 - 08/24/05 01:33 AM (19 years, 4 months ago)

Well, this new batch is with new jars and they were cool. The temp is about 80-82 right now, not even a sign of white anywhere.


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InvisibleFooMan
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Registered: 02/02/05
Posts: 8,957
Loc: Earth Flag
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Re: cambodian strains.. not growing [Re: hollisatp]
    #4573993 - 08/24/05 06:24 AM (19 years, 4 months ago)

Did you follow the tek to a "T" and how was your syringe stored between these jars and your last batch? There are a lot of possibilities for what could be going wrong. Who did you purchase the spores from?


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Quick WBS Prep

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