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InvisiblePsilocybeSamurai
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Registered: 08/19/05
Posts: 32
Muti spore culture difficulty.
    #4564242 - 08/21/05 08:11 PM (18 years, 7 months ago)

Greetings everyone, I have a question about some germinating Mexi Cub spores I have on MEA. First of all, I've been growing shrooms for awhile now using poor man techniques but recently I've upgraded myself to more advanced methods. I'm currently working with 5 strains right now including Mexi Cubs, Chinese Hunan, Mezatepic, Penis Envy and Paneolus Tropicalius. I did a multi spore culture w/ the Mexi Cubs on 8 petris on MEA. Now they're 2 weeks along and mycelium is all over the place, but no rhizomorphic at all, there is only cottony and longitudinally radial fan like stuff that dosen't look all that great. At least there's no contamination. Now, should I wait longer? is it going to change? cuz I'm ready to scrap this batch and move on to another one of my strains. This is my second try with the Mexis and it's just not producing that healthly looking rhizomorphic growth that we all need!! so how long does a multi spore culture take to rhizomrph? or is this a case of weak spores.



Thank u for your time.

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InvisibleThumpaCap
Beer Taster

Registered: 02/19/05
Posts: 568
Loc: Shroomwhere Out There
Re: Muti spore culture difficulty. [Re: PsilocybeSamurai]
    #4564524 - 08/21/05 09:06 PM (18 years, 7 months ago)

:shrug:


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Invisiblekrishnamurti
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Registered: 06/27/05
Posts: 382
Re: Muti spore culture difficulty. [Re: ThumpaCap]
    #4565063 - 08/21/05 10:57 PM (18 years, 7 months ago)

id say just find the healthiest looking piece of myc and spawn it to a Liquid Culture. innoc that to whatever until see rhiz grow then put some of that in an LC or petri dish. or maybe im just talking out of my ass. i dunno.  :tongue:


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 10 months
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Re: Muti spore culture difficulty. [Re: krishnamurti]
    #4569931 - 08/23/05 09:02 AM (18 years, 7 months ago)

Sometimes it can be quite linear (longitudinally radial fan) and hard to recognize. I have found isolating the fastest growth from several areas will give you some substrains to choose from. These often will become quite ropey (rhizo) when substrates are changed either to PDA or to grains. GL


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Getting the most out of your casings!, A pinning strategy.
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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InvisibleMagash
Da Bud Guru
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Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
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Re: Muti spore culture difficulty. [Re: PsilocybeSamurai]
    #4570205 - 08/23/05 10:17 AM (18 years, 7 months ago)

Not one of these had rhizo growth. I use to care about it but now I could give a shit.


jar full of cotton

made into a coton growth casing

Then into cotton growth shrooms

Don't throw out your work.


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InvisiblePsilocybeSamurai
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Registered: 08/19/05
Posts: 32
Re: Muti spore culture difficulty. [Re: Magash]
    #4572607 - 08/23/05 08:42 PM (18 years, 7 months ago)

Ya'know, your right. I have prime mycelium in these petris, I HAVE WORK TO DO! And I'll show pics of the results, oh yes...oh yes. :mushroom2:

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