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OfflineTeRzMaStA
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Registered: 10/08/01
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Colonizing
    #456636 - 11/12/01 11:41 PM (22 years, 4 months ago)

AFOAF has 36 1/2 pint verm/brf jars he inocualted last sunday and when he looked at them today 32 of them had large white spots of mycelium(sp). None of the 36 jars have contams and 12 jars he didn't have enough spore water to inoculate r fine too. He was pretty happy, and told me to post this for him.


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OfflineTeRzMaStA
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Re: Colonizing [Re: TeRzMaStA]
    #456637 - 11/12/01 11:42 PM (22 years, 4 months ago)

If I can find some batteries i'll post some pics for him


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OfflineXxMuSHRooMHeaDxX
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Registered: 10/16/01
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Re: Colonizing [Re: TeRzMaStA]
    #456850 - 11/13/01 09:38 AM (22 years, 4 months ago)

what strains?

XX


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OfflineTeRzMaStA
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Re: Colonizing [Re: XxMuSHRooMHeaDxX]
    #457082 - 11/13/01 02:57 PM (22 years, 4 months ago)

they are all ecuador cubensis. Do u know if the age of the substrate makes any difference? my friend has 12 jars that weren't inoculated and they didn't get contamed. they have been sitting around for a little over a week. could he use them in a week or two when he gets more spores or should he just make some new cakes?


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OfflineGroove_N
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Registered: 09/22/01
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Re: Colonizing [Re: TeRzMaStA]
    #457100 - 11/13/01 03:14 PM (22 years, 4 months ago)

TeRzMaStA - RE: Aged Substarte:
Are the jars hole still covered (tinfoil)? If so contams should not be able to get to the substrate. There are two thoughts on your situation.
1. Start over - newer is better
2. Your substrate is in good condition without contams. This is good, because you know that if you get contams, it most likely came from innoculation.

I wouldl use the jars.

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OfflineTeRzMaStA
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Re: Colonizing [Re: Groove_N]
    #457366 - 11/13/01 07:46 PM (22 years, 4 months ago)

they have filter discs on them. they aren't contamed. He will probably use them. thanks for the help


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Edited by TeRzMaStA (11/26/01 08:56 PM)

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Offlinesylo
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Re: Colonizing [Re: TeRzMaStA]
    #457732 - 11/14/01 03:03 AM (22 years, 4 months ago)

When you say "last Sunday" do you mean the eleventh? It takes about four days for spores to germinate and another day or two before you can expect to see growth. Unless you inoculated with live mycelium, any sign of growth before four days is highly likely to be a contam. Are you sure you arn't growing mold?

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OfflineMuShi_KiNg
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Registered: 09/25/01
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Re: Colonizing [Re: sylo]
    #457984 - 11/14/01 10:39 AM (22 years, 4 months ago)

the problem Might be :) that if you use the same Jar's they will become dry :)
just imo :)

36 Jars hectic Brother YOu cultivating for the nation
Say you get 4 dry grams per jar 144 Dry grams Cool :)
Why do you do so many ? YOu should look into some mass production

I think i spoke to much already
Tell your freind i say hi

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OfflineTeRzMaStA
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Re: Colonizing [Re: sylo]
    #458234 - 11/14/01 03:01 PM (22 years, 4 months ago)

I'm sure that it isn't mold. He inoculated them on the 4th.


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Edited by TeRzMaStA (11/14/01 03:07 PM)

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OfflineTeRzMaStA
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Re: Colonizing [Re: MuShi_KiNg]
    #458238 - 11/14/01 03:06 PM (22 years, 4 months ago)

My FOAF made so many cause he can't kep doin it for a long preiod of time and wanted to get a lot out of it. What exactly should he look into?


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OfflineTeRzMaStA
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Re: Colonizing [Re: sylo]
    #458880 - 11/15/01 12:39 AM (22 years, 4 months ago)

here is a pic

you can't really see from the picture but every jar except 1 has mycellium growth.


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OfflineTeRzMaStA
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Re: Colonizing [Re: TeRzMaStA]
    #460727 - 11/16/01 06:12 PM (22 years, 4 months ago)

Here is an updated pic


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InvisibleOlgualion
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Re: Colonizing [Re: TeRzMaStA]
    #460731 - 11/16/01 06:22 PM (22 years, 4 months ago)

Go ahead and innoc your waiting sterilized jars. There should be no prob. Dude says he has waited over a month before innoc'ing jars before w/o any problems. As long as the tape is still on them and the lids are tight there should be minimal(if any) loss of moisture.


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OfflineTeRzMaStA
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Re: Colonizing [Re: TeRzMaStA]
    #470431 - 11/26/01 07:32 PM (22 years, 4 months ago)

a couple updated pictures








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OfflineSnobrdr311
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Re: Colonizing [Re: TeRzMaStA]
    #470474 - 11/26/01 08:13 PM (22 years, 4 months ago)

why do you have them upside down? wouldn't that limit gas exchange?

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OfflineTeRzMaStA
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Re: Colonizing [Re: Snobrdr311]
    #470477 - 11/26/01 08:17 PM (22 years, 4 months ago)

My friend was told to flip them and make a space between the cake and the jar so there is an air pocket around the bottom of the cakes. that that will help the bottoms of the cakes colonize faster.


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Edited by TeRzMaStA (11/26/01 08:59 PM)

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OfflineSnobrdr311
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Re: Colonizing [Re: TeRzMaStA]
    #470480 - 11/26/01 08:21 PM (22 years, 4 months ago)

yeah I guess maybe, but if there's no gas exchange the mycelium will stall, I wouldn't leave them upside down longer than a day or two.

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OfflineSnobrdr311
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Re: Colonizing [Re: TeRzMaStA]
    #470482 - 11/26/01 08:25 PM (22 years, 4 months ago)

what temps are you keeping them at?

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OfflineTeRzMaStA
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Re: Colonizing [Re: Snobrdr311]
    #470483 - 11/26/01 08:26 PM (22 years, 4 months ago)

it has only been a day. can someone who has experience doing this tell me what to do so I can tell my friend.


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Edited by TeRzMaStA (11/26/01 09:02 PM)

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OfflineSnobrdr311
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Re: Colonizing [Re: TeRzMaStA]
    #470485 - 11/26/01 08:27 PM (22 years, 4 months ago)

do I sound like I don't have any experience? lol...

you're doing fine dude, it shouldn't be that much longer and they'll be fully colonized. are you going to case any of them? i'd highly suggest it considering you have so many jars.

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