|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
BenKenobi
Enlightened 1


Registered: 05/22/05
Posts: 342
Loc: Europe
Last seen: 11 years, 8 months
|
Any way to speed up LC colinsation?
#4565700 - 08/22/05 02:33 AM (19 years, 4 months ago) |
|
|
I made my first LC about 2 or more weeks ago usign jars similar to how agar does it with the jar and two tubes filled with polyfill. The LC was a 3-4% Kara mix.
After bout 2-3days i notcied the first signs of growth and it was lookin like it was doing ok for the first week but since then i havent noticed hardly any more growth. The temps havent changed in the TIT and the jars in there with them are colinising just as fast. I couldnt seem to locate a post where it had the total colinsation time for a LC so dunno if its going slow or not.
Perhaps it is runnin out of O2, maybe polyfill packed in too tight, anyone recommend i remove it and inject some air in thier with a shyringe, i just dun wanna help increase any chances of contams. It wouldnt of run out of sugar to feed on would it?
Cheers
-------------------- Old Ben
I beleive that my job in mappin the impulse pathways of a single neuron can allow us to open a mecca into the quailitative functions of the human geno...
Please support your local free spore ring by sendin back prints from successful grows, FSRE
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Any way to speed up LC colinsation? [Re: BenKenobi]
#4565865 - 08/22/05 05:12 AM (19 years, 4 months ago) |
|
|
 Stir Bar & stir plate speeds up the process.
Just agitate the LC jars a bit.
--------------------
|
kronnyQ
SuperstudExtraordinaire


Registered: 07/22/04
Posts: 2,488
Loc: Anytown USA
Last seen: 5 months, 4 days
|
Re: Any way to speed up LC colinsation? [Re: agar]
#4565874 - 08/22/05 05:23 AM (19 years, 4 months ago) |
|
|
Those stir plates are gnarly, how much do they usually run?
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Any way to speed up LC colinsation? [Re: kronnyQ]
#4565886 - 08/22/05 05:35 AM (19 years, 4 months ago) |
|
|
Between $20 & $40 on eBay + shipping, if you are patient. Search in lab eqt under stir plate & hot plate , sometimes you can get a combo cheaper than a straight stir plate.
http://cgi.ebay.com/Newer-Corning-Hot-Pl...1QQcmdZViewItem
--------------------
Edited by agar (08/22/05 05:52 AM)
|
FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
|
Re: Any way to speed up LC colinsation? [Re: agar]
#4565921 - 08/22/05 06:38 AM (19 years, 4 months ago) |
|
|
I like the new avatar Agar!
--------------------
Quick WBS Prep
|
BenKenobi
Enlightened 1


Registered: 05/22/05
Posts: 342
Loc: Europe
Last seen: 11 years, 8 months
|
Re: Any way to speed up LC colinsation? [Re: FooMan]
#4566210 - 08/22/05 10:54 AM (19 years, 4 months ago) |
|
|
Yer i have been stiring it up each mornin and it breaks it up into bout 10 lil peices but not long b4 its back to the same 3cm big clump.
Whats the average time for a karo culture to colinise to the point where it is ready to be used?
|
thenewguy05
The Mushroom Man


Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
Last seen: 3 years, 5 months
|
Re: Any way to speed up LC colinsation? [Re: BenKenobi]
#4566222 - 08/22/05 11:03 AM (19 years, 4 months ago) |
|
|
I usually use mine up between week 2-3.
|
Hotnuts
old hand


Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 1 day, 15 hours
|
Re: Any way to speed up LC colinsation? [Re: thenewguy05]
#4566435 - 08/22/05 12:30 PM (19 years, 4 months ago) |
|
|
Mine take about 4-7 days to be good and colonized. Then I store them in a refridgerator....
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 786 topic views. 33 members, 160 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|