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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Perlite, Vermiculite

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Offlinethelonederanger
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Registered: 08/20/05
Posts: 6
Last seen: 8 years, 5 months
9 days, no germination
    #4559410 - 08/20/05 04:11 PM (11 years, 10 months ago)

i followed PF Tek for Simple Minds to the dot: http://www.fungifun.org/pf/pf_en.htm

inoculated on Aug 11 and got syringe of Golden Teacher from Spore Works
i shook the syringe alot and injected against glass as well

have not seen any change at all...

can anyone help please?


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OfflineJellyRollMorton
Stranger Danger
Registered: 07/08/05
Posts: 116
Loc: Bobland
Last seen: 3 months, 1 day
Re: 9 days, no germination [Re: thelonederanger]
    #4559431 - 08/20/05 04:15 PM (11 years, 10 months ago)

could just be a case of bad spores. What incubation temps?


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Offlinethelonederanger
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Registered: 08/20/05
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Re: 9 days, no germination [Re: JellyRollMorton]
    #4559452 - 08/20/05 04:18 PM (11 years, 10 months ago)

78-80 degrees F

i have left the alum shield on though... took em off today


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OfflineJellyRollMorton
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Re: 9 days, no germination [Re: thelonederanger]
    #4559459 - 08/20/05 04:20 PM (11 years, 10 months ago)

Only thing i can think of is bad spores. try making a liquid culture with your next syringe.


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Offlinethelonederanger
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Registered: 08/20/05
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Re: 9 days, no germination [Re: JellyRollMorton]
    #4559543 - 08/20/05 04:44 PM (11 years, 10 months ago)

can i reuse my jars+substrate if they have no contams?

Spore Works stuff should be good


Edited by thelonederanger (08/20/05 04:45 PM)


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OfflineJellyRollMorton
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Registered: 07/08/05
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Re: 9 days, no germination [Re: thelonederanger]
    #4559559 - 08/20/05 04:47 PM (11 years, 10 months ago)

As long as they haven't dried out they should be fine. But make sure there is no growth before re-using. Putting two different strtains in a jar will make them compete.


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Offlinespaceman
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Registered: 07/09/05
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Re: 9 days, no germination [Re: JellyRollMorton]
    #4559784 - 08/20/05 05:56 PM (11 years, 10 months ago)

Did you pack your jars or just let the substrate fall into the jar? It makes a big difference. Some of the jars that I packed, I didnt know any better, took a couple weeks to start colonizing. And total colonization took over a month.


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Offlinethelonederanger
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Re: 9 days, no germination [Re: spaceman]
    #4559870 - 08/20/05 06:29 PM (11 years, 10 months ago)

substrate is in there very fluffy

it may be that they were stored in small cabinet w/ foil lids
which = bad gas exchange

i will wait 1 more week and then email Spore Works


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Offlinethelonederanger
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Re: 9 days, no germination [Re: thelonederanger]
    #4560655 - 08/20/05 10:27 PM (11 years, 10 months ago)

well if the syringe was bad, how do I ask spore works for a new one

since we are not supposed to be growing them in the first place...

EDIT: realized i used perlite/brf substrate vs verm/brf substrate, would that make a difference?


Edited by thelonederanger (08/20/05 10:36 PM)


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Offlinethelonederanger
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Re: 9 days, no germination [Re: thelonederanger]
    #4560696 - 08/20/05 10:38 PM (11 years, 10 months ago)

yup i botched it...

time to start over...


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Offlineasanaman
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Registered: 08/11/05
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Re: 9 days, no germination [Re: thelonederanger]
    #4562011 - 08/21/05 11:06 AM (11 years, 10 months ago)

So did you give up?
I have a similar problem(also 11August, also GT), only 2/6 jars show growth.
Are you going to contact your supplier for a new syringe?

Good luck anyway.


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InvisibleOldSpice
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Registered: 08/25/03
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Re: 9 days, no germination [Re: thelonederanger]
    #4562058 - 08/21/05 11:38 AM (11 years, 10 months ago)

Send a e-mail to them, everyone makes mistakes, get some verm also for your next batch


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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InvisiblePrisoner#1
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Re: 9 days, no germination [Re: thelonederanger]
    #4562085 - 08/21/05 11:49 AM (11 years, 10 months ago)

Quote:

thelonederanger said:
well if the syringe was bad, how do I ask spore works for a new one




let them know that the spores did not appear to be viable

Quote:

EDIT: realized i used perlite/brf substrate vs verm/brf substrate, would that make a difference?




given that the mistake was yours I hope you decide to simply buy a new one


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: 9 days, no germination [Re: thelonederanger]
    #4562098 - 08/21/05 11:54 AM (11 years, 10 months ago)

I save a lot of money becausee i switched to www.ralphstersspores.com  :smirk:


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Perlite, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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