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Offlinesexymoreno
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Registered: 08/04/05
Posts: 169
Last seen: 10 years, 8 months
Fruiting contaminated cake.
    #4555039 - 08/19/05 12:30 PM (11 years, 3 months ago)

yesterday i was supser mad cuz all my jars got infected with blue green mold, so i put it in the dark to keep light from reaching it, since there would be no light, the mold would have no energy to grow.

but i used my whole syringe on these contaminated jars, and then the rest of it on some experiments, so basically im out of spores. i was thinking if i could fruit the contaminated cakes just to get one mushroom or two and then take spores from that?

and wen i pick the shrooms to take prints from, should i wash it first or will the spores get washed away?


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Invisiblewoodzmun
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Registered: 07/14/04
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Re: Fruiting contaminated cake. [Re: sexymoreno]
    #4555049 - 08/19/05 12:36 PM (11 years, 3 months ago)

Mold will certainly grow in the dark, and I'm sad to say even if the contamed cakes do fruit, those contam mold spores will be all over the fruits. Washing won't help either so I would say it's back to the drawing board. Have you figured out why this batch contamed?


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OfflineRamuh
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Registered: 07/04/05
Posts: 837
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Re: Fruiting contaminated cake. [Re: sexymoreno]
    #4555050 - 08/19/05 12:37 PM (11 years, 3 months ago)

Just as a note, mold tends to like the darker places, so its not like you can quite starve it of light. Mushrooms technically don't need any light at all to grow either (just gets used as a marker for what is up).

Now, the fact that ALL of your jars got contaminated perhaps should lead you to look at your sterlizing techniques first. Chances are if you have 0% success at getting a sterile myc growing enviornment, there is no way you'll be able to get a good clean sporeprint anyway.

Did any myc grow at all? If tha answer is no, well then even if you could birth it, there would be nothing there that would even have a chance to fruit. If there is any good mycellium still, you may be able to transplant some of it I think (Im not very familiar with transplaning, so someone correct me if Im wrong).

Basically, sorry, but it looks like you should just start over again, and figure out what went wrong in the sterlizing process.


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Offlinethenewguy05
The Mushroom Man
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Registered: 02/11/05
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Re: Fruiting contaminated cake. [Re: Ramuh]
    #4555057 - 08/19/05 12:44 PM (11 years, 3 months ago)

too bad about your crop, i just lost an entire 66 qt bin due to someone Else's negligence so i feel your pain. My best suggestion is a longer sterilization period and more sterile techniques. use alcohol religiously. use it in between every inoculation site. good luck on the next grow.
P.S. what strain where you using.


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Offlinesexymoreno
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Registered: 08/04/05
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Re: Fruiting contaminated cake. [Re: thenewguy05]
    #4555069 - 08/19/05 12:51 PM (11 years, 3 months ago)

im pretty sure it's got something to do with condensation on the inside walls of the jar... that's where all the blue started coming from. and yes, newguy, i did use alcohol religiously, i could've sacrificed a cow to it lol.

i definitely got mycelium, this is the last week that i actually had to wait before that 3-5 day wait til i birth it. then i got too excited and tried putting that open jar of creme developer, if you saw my other post about oxygen. the liquid evap'd and then got inside the jars.

grrr.... i hope the mailman don't take my money, i requested a spore print from fsrc. if he/she did then i'll have to wait it out 2 weeks on paypal 8-) and the thing about the fsrc is that i said i'd send a print for matias romero cuz that's what i had but then i can't now. dang....

i did, however take one of the contaminated cakes, and took the mcyelium from one side than cased it. now im waiting.... anyone think this won't work?


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Offlinesexymoreno
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Re: Fruiting contaminated cake. [Re: sexymoreno]
    #4555073 - 08/19/05 12:54 PM (11 years, 3 months ago)

and i also put some of the myc on agar and it seems to be doing great today.


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OfflineBoringNickName
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Registered: 06/16/05
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Re: Fruiting contaminated cake. [Re: sexymoreno]
    #4555086 - 08/19/05 01:02 PM (11 years, 3 months ago)

Look up the chronic tek, if the contamination isn't too bad you might be able to get some fruits. However, it's really not worth messing around with in the long run.


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Invisibleorchidfanatic
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Re: Fruiting contaminated cake. [Re: BoringNickName]
    #7313614 - 08/20/07 11:30 AM (9 years, 3 months ago)

what if you were to fruit a contaminated cake .. grow a shroom
and clone a tissue from the shroom put that in peroxide to kill the mold and then clone the tissue on some agar or such would that work?
one of my cakes is contaminated so I wanted to try a sea of shroom tek
and clone any shroom that comes up ..??


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OfflineVegan
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Re: Fruiting contaminated cake. [Re: orchidfanatic]
    #7313979 - 08/20/07 01:19 PM (9 years, 3 months ago)

if I was to do anything with a contaminated cake ..I would throw it in the garbage..

don't put anything in your FC with contaminates.. Unless you want to go back and sterilize the whole thing when you get cakes with no mold..
IMHO


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Invisibleorchidfanatic
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Re: Fruiting contaminated cake. [Re: Vegan]
    #7316896 - 08/21/07 09:41 AM (9 years, 3 months ago)

I might just throw it outside put some peat and verm casing on it and see what happens .. RH is very high here in FL but the temps are pretty hot .. doesn't stop these shrooms though ..


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