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Offlinelemming
evil

Registered: 10/28/01
Posts: 107
Last seen: 12 years, 3 months
Quick way to test spore vitality
    #4550617 - 08/18/05 07:24 AM (12 years, 3 months ago)

Hey all, it's been quite a while...
Just a quick question really, it's surely been answered other times but I searched for the last half hour and really couldn't find anything...
I have a few spore syringes dating a few years back, they surely haven't been kept in the proper conditions, but I was still wondering if there was a quick and dirty way to just see if one of the syringes may perhaps still be "active".
maybe something that doesn't involve pressure cooking, I don't want to start a culture just need to know if the spores are still alive.
thanks a bunch


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Invisiblebackupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: Quick way to test spore vitality [Re: lemming]
    #4550624 - 08/18/05 07:26 AM (12 years, 3 months ago)

well a quick dirty lc, mix it up microwave it for a bit and then shoot some spores in there. give it some time to see if they grow.
you really should go about it in a good sterile way, cause i would imagine them to still be viable, at least some of them anyway. why waste em?
peace


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Offlinelemming
evil

Registered: 10/28/01
Posts: 107
Last seen: 12 years, 3 months
Re: Quick way to test spore vitality [Re: backupwards]
    #4550646 - 08/18/05 07:42 AM (12 years, 3 months ago)

please forgive my ignorance, but what do you mean with "lc" ? I don't understand what I need to mix up and microwave...
and since most of the syringes are quite still full, I intend to shoot just a small part of the solution, because they are 2-3 years old and have been kept in a box on a shelf, so I wouldn't be too sure...
thanks


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Offlineluckytriple6
spun, confused, and needing hugs
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Registered: 08/25/03
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Re: Quick way to test spore vitality [Re: lemming]
    #4550670 - 08/18/05 08:19 AM (12 years, 3 months ago)

He meant liquid culture, like watered down karo or honey. heres a link given to me by Roadkill
http://www.shroomery.org/forums/showflat.php/Number/4198734#Post4198734
I hope that helps. I had a syringe for about a year kept in an attic and was still able to use it....took a long time to colonize though.


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I'm in outer space
I've just vanished without a trace
I'm going to a pretty place now where the flowers grow
I'll be back in an hour or so

[quote]Abuse said:
the dea can go fuck themselves! with the internet, the impossible is possible![/quote]


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Offlinelemming
evil

Registered: 10/28/01
Posts: 107
Last seen: 12 years, 3 months
Re: Quick way to test spore vitality [Re: luckytriple6]
    #4550703 - 08/18/05 09:03 AM (12 years, 3 months ago)

thanks a bunch!
would you happen to know a good honey:water ratio?
it only mentions karo:water ratio in that thread.
thanks again :wink:


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InvisibleRoadkillM
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Registered: 12/11/01
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Re: Quick way to test spore vitality [Re: lemming]
    #4550708 - 08/18/05 09:08 AM (12 years, 3 months ago)

the same ratio will work for honey and karo.

tc


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You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


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