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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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InvisibleImorph
journeyman
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Registered: 11/10/01
Posts: 146
opinions needed
    #454508 - 11/10/01 10:28 PM (22 years, 10 months ago)

hello all i have from time to time i scan theses forums and in the past dd. i have several quests that someone probably knows. (1) whats the problems using beans or alfalfa as substrates? (2)whats the tek for recovering viable use from a dubious trade print? Imorph

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Offlinesylo
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Registered: 10/29/00
Posts: 219
Last seen: 22 years, 5 months
Re: opinions needed [Re: Imorph]
    #454543 - 11/10/01 11:08 PM (22 years, 10 months ago)

(1) Shroom substrates all have a high carbon to nitrogen ratio. Lots of carbon in the form of starch or cellulose but little nitrogen in the form of protein or other nitrogen compounds. Shrooms and other fungi require little nitrogen to grow while bacteria require large amounts. The lack of nitrogen gives shrooms a competative advantage over bacteria. Beans and alfalfa contain large amounts of nitrogen in the form of protein. Protein is good for shrooms but even better for bacteria so contams are a problem.
(2)To get clean mycelium from a bad print, put a few spores on the agar with a flame sterilized wire loop and spread the spores as far apart as possible. This must be done gently so as not to tear up the agar surface. When the spores germinate, pick a chunk of clean mucelium and place it on another agar plate leaving the contams behind. It is often necessary to repeat the process to get a pure mycelium culture.

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InvisibleImorph
journeyman
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Registered: 11/10/01
Posts: 146
Re: opinions needed [Re: sylo]
    #454617 - 11/11/01 12:47 AM (22 years, 10 months ago)

Oh.... ok, i just wondered. i remember breif talk concerning those topics way back when. unfortunately in my area a lot of hay/straw bales contain alfalfa. i ll also include a little ho2o2 in re purification rites. thx you * SYLO

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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