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OfflineSillypcybin
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Registered: 08/16/05
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Cloning fruit bodies
    #4544786 - 08/17/05 01:56 AM (11 years, 3 months ago)

I have cloned fruit bodies on agar as well as in nutritive sterile water.  With agar, I have also singled out the best rhizo's where it ended up creating just thick ropes after a few transfers. 

With throwing a piece of fruit body in nutritive water, is that true cloning? Or does it just create more mycorhizzae which isnt a true clone of the original?

Working with petri dishes/agar in a sterile environemt is difficult.  H2O2 in nutritive water and agar makes it a bit easier since it doesnt kill the mycelium... but it's still a bitch! :smile:

Anyone have some cool mushroom cloning methods????


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OfflineWBecklund
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Re: Cloning fruit bodies [Re: Sillypcybin]
    #4544931 - 08/17/05 02:25 AM (11 years, 3 months ago)

Quote:

Sillypcybin said:


With throwing a piece of fruit body in nutritive water, is that true cloning? Or does it just create more mycorhizzae which isnt a true clone of the original?






Yes, unless there are spores on it. It would be true cloning if it where off the stem of an fruit with an unbroken veil.

It would preferably be off a flush that none of the fruit had broken their veils yet, otherwise you run the risk of multispore.


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OfflineSillypcybin
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Re: Cloning fruit bodies [Re: WBecklund]
    #4544960 - 08/17/05 02:30 AM (11 years, 3 months ago)

To me, it doesntmatter if the veil is broken. The flesh I use is a carving from the center of the stem. I slice the stem in half and carve out a small amount of flesh. Untouched by spores or anything. I've experimented with using the flesh of the cap but it brings the same exact results.

It's too bad you cannot single out the strain any further in nutrient water as you can with petri dishes/agar :/


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OfflineWBecklund
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Re: Cloning fruit bodies [Re: Sillypcybin]
    #4544978 - 08/17/05 02:32 AM (11 years, 3 months ago)

You could isolate a strain, but it would be hard to isolate the most rhizomorphic aspects of it.


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OfflineSillypcybin
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Re: Cloning fruit bodies [Re: WBecklund]
    #4544988 - 08/17/05 02:34 AM (11 years, 3 months ago)

I meant isolate :laugh: And how would you do this in a nutrient solution? Just aim the syringe at the ropiest section?


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OfflineSillypcybin
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Re: Cloning fruit bodies [Re: Sillypcybin]
    #4544997 - 08/17/05 02:36 AM (11 years, 3 months ago)

Also, I have read a few years back that adding h2o2 in a developed mycelial solution or even having it in there when dumping a fruit body/mycelium in there helps it develop resistance to mold/bacteria? Or maybe that was just while it was in the solution. That seems more probable.


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OfflineWBecklund
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Re: Cloning fruit bodies [Re: Sillypcybin]
    #4545001 - 08/17/05 02:36 AM (11 years, 3 months ago)

The best way I could think of is innoculating agar from the LC, and isolating on the Agar, though, I would imagine that you could get a decent isolate via careful syringe aiming :smile:


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OfflineSillypcybin
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Re: Cloning fruit bodies [Re: WBecklund]
    #4545016 - 08/17/05 02:42 AM (11 years, 3 months ago)

What I used to do is have H2O2 agar ready on a dish and cut the flesh of the largest fruit in the flush and drop a small piece on there. Then I would find the ropiest section and cut that, put that on a new dish. I would do this several times until in the end result, the last petri dish looks like it had a thick spider web in it. The flushes from that were ridiculous.


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OfflineWBecklund
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Re: Cloning fruit bodies [Re: Sillypcybin]
    #4545022 - 08/17/05 02:44 AM (11 years, 3 months ago)

That sounds like a good technique, but remember, you are cloning the whole mycelium, not just 1 fruit, so you're better off looking for the biggest flushes instead of the biggest fruit.


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OfflineSillypcybin
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Re: Cloning fruit bodies [Re: WBecklund]
    #4545047 - 08/17/05 02:50 AM (11 years, 3 months ago)

Good point. I think I'm too used to cloning marijuana. Ahh, those were the days.

Anyway, the flushes seem to always be equal between my casings. I always thought any variation was due to variation in airflow, humidity and moisture content of the casings.


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Offlineweaksause
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Re: Cloning fruit bodies [Re: Sillypcybin]
    #20618567 - 09/25/14 08:06 PM (2 years, 2 months ago)

so what happens with : maturing pin flesh with spores in agar ?


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OfflineDekozn
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Re: Cloning fruit bodies [Re: weaksause]
    #20618873 - 09/25/14 09:08 PM (2 years, 2 months ago)

Really a 9 year old thread with bad advise? Let it die
H2O2 doesn't help do anything except slowing down growth


--------------------
Organized people are just to lazy to search for their stuff...


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OfflinecronicrM
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Re: Cloning fruit bodies [Re: Sillypcybin]
    #20619047 - 09/25/14 09:08 PM (2 years, 2 months ago)

This thread has been closed.

Reason:
:facepalm:


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