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Offlinegreatlakes
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Registered: 07/25/05
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Last seen: 10 years, 4 months
WBS?
    #4544173 - 08/16/05 11:44 PM (11 years, 3 months ago)

OK well i am pcing my wbs and i have 7 more jars to do tonight but i am damn tired,I soaked them for 14 hours then simmered.my question is this can i wait till tomorrow around 4:00pm to do the other jars or will they spout out?or something else go bad with them?


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Invisibleagar
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Re: WBS? [Re: greatlakes]
    #4544188 - 08/16/05 11:48 PM (11 years, 3 months ago)

14 hours is a bit short - for a WBS soak. 24 is far better & 36 doesnt hurt. Sure, you can wait until tomarrow. I would have the remaining WBS - soaking - during that time, if I were you.


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Offlinegreatlakes
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Re: WBS? [Re: agar]
    #4544213 - 08/16/05 11:53 PM (11 years, 3 months ago)

humm,not to disagree with you but was the same amount of time that i did the last time and all 12 was done in 11 day with no contams,,,,but i will soak the rest till tomorrow since it is you who is saying that's not long enough.Out of curiosity why would you say that's not long enough is it because the bad spore have not spouted yet or is it something to do with the moister?


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Offlinegreatlakes
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Re: WBS? [Re: greatlakes]
    #4544218 - 08/16/05 11:54 PM (11 years, 3 months ago)

o ya by the way thanks for that info i will soak longer for now on


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Anonymous

Post deleted by Anno [Re: greatlakes]
    #4544227 - 08/16/05 11:57 PM (11 years, 3 months ago)



Edited by Anonymous (08/16/05 11:57 PM)


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Offlinegreatlakes
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Re: WBS? [Re: ]
    #4544291 - 08/17/05 12:10 AM (11 years, 3 months ago)

well in agar defence it does say 12 hours SOMETIMES works
(12 hours works sometimes, 24 hours works, so does 36 hours.
The point of this post is - time isn?t ALL that important.)
And after going over my notes i did soak for 18 hours last time
Anyhow i'm soaking them till 4:00pm tomorrow which will make a total of 30 hours so i should be ok


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Offlinegreatlakes
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Re: WBS? [Re: greatlakes]
    #4544322 - 08/17/05 12:16 AM (11 years, 3 months ago)

i wonder with the one i got in the pc now(the ones i soaked for 14 hours)if i should pc them for longer then normal?


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OfflineBasidiomycete
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Re: WBS? [Re: greatlakes]
    #4544362 - 08/17/05 12:25 AM (11 years, 3 months ago)

How long is normal for you? 90min is what i always did...


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Ginger bread


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Offlinegreatlakes
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Re: WBS? [Re: Basidiomycete]
    #4544367 - 08/17/05 12:27 AM (11 years, 3 months ago)

75 mins i did the last time,,,had no problems


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Offlinegreatlakes
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Re: WBS? [Re: greatlakes]
    #4544379 - 08/17/05 12:29 AM (11 years, 3 months ago)

humm u said "did" as in the past what do you spawn with now?


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OfflineBasidiomycete
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Re: WBS? [Re: greatlakes]
    #4544402 - 08/17/05 12:34 AM (11 years, 3 months ago)

afoaf still does the same thing my friend... never gets contams. the WBS gets soaked for 24hrs, simmered for 20min, let drain for 6hrs, put into jar, tyvek then band with 3 1/4" holes stuffed with polyfill, shake at 30%, fully colonized on average at 8 or 9 days, have gotten down to 6 days though. afoaf always uses LC innoculant as well. incubates at a constant 84F, with a 65% rH.

GL to ya


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Ginger bread


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Offlinegreatlakes
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Re: WBS? [Re: Basidiomycete]
    #4544411 - 08/17/05 12:36 AM (11 years, 3 months ago)

ya thanks for the info
later man


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